Increased osmolality enhances the tight junction-mediated barrier function in a cultured renal epithelial cell line

2018 ◽  
Vol 43 (1) ◽  
pp. 73-82 ◽  
Author(s):  
Leandro P. Canuto ◽  
Carla B. Collares-Buzato
Keyword(s):  
Epithelial Cell ◽  
Tight Junction ◽  
Cell Line ◽  
Barrier Function ◽  
Epithelial Cell Line ◽  
Renal Epithelial Cell ◽  
Renal Epithelial Cell Line

Electrophysiological Measurements of a Toad Renal Epithelial Cell Line (A6) as an Assay for Predicting Ocular Eye Irritancy

1992 ◽  
Vol 20 (2) ◽  
pp. 218-221
Author(s):  
Henning F. Bjerregaard
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
South African ◽  
Mdck Cells ◽  
Epithelial Cell Line ◽  
Renal Epithelial Cell ◽  
Electrophysiological Measurements ◽  
Renal Epithelial Cell Line ◽  
Darby Canine Kidney

An established epithelial cell line (A6) from a South African clawed toad (Xenopus laevis) kidney was used as a model for the corneal epithelium of the eye in order to determine ocular irritancy. When grown on Millipore filter inserts, A6 cells form a monolayer epithelium of high electrical resistance and generate a trans-epithelial potential difference. These two easily-measured electrophysiological endpoints showed a dose-related decrease after exposure for 24 hours to seven selected chemicals of different ocular irritancy potential. It was demonstrated that both trans-epithelial resistance and potential ranked closely with in vivo eye irritancy data and correlated well (r = 0.96) with loss of trans-epithelial impermeability of Madin-Darby canine kidney (MDCK) cells, detected by use of a fluorescein leakage assay.

scholarly journals Expression of phosphoenolpyruvate carboxykinase (PEPCK) chimeras in renal epithelial cells. Retention of appropriate physiological responsiveness using enhancerless retroviral vectors

1992 ◽  
Vol 284 (3) ◽  
pp. 725-732 ◽  
Author(s):  
A S Pollock ◽  
D H Lovett
Keyword(s):  
Epithelial Cell ◽  
Cyclic Amp ◽  
Cell Line ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Large Fraction ◽  
Expression System ◽  
Retroviral Vectors ◽  
Epithelial Cell Line ◽  
Renal Epithelial Cell ◽  
Renal Epithelial Cell Line

We used an enhancerless U3 mutant retroviral vector to deliver chimeras of the phosphoenolpyruvate carboxykinase (PEPCK) promoter region to a renal epithelial cell line capable of expressing PEPCK mRNA. Chimeras consisting of the PEPCK promoter and chloramphenicol acetyltransferase, neomycin phosphotransferase or human growth hormone genes were expressed after viral infection of the NRK52E renal epithelial cell line. Virus-delivered sequences in which the direction of PEPCK promoter transcription was antegrade to the normal direction of the long terminal repeat (LTR)-initiated transcription correctly upon stimulation with dexamethasone or 8-bromo cyclic AMP and upon lowering of the extracellular pH. Fluorescent primer extension in situ using primers specific for virus-delivered sequences of antegrade constructs indicated that a large fraction of NRK52E cells could be infected by co-cultivation with virus-producing psi-2 cells without G418 selection. Virus-delivered constructs whose orientation was opposite to that of the LTRs were expressed at very low levels, with transcripts detectable by PCR only in RNA from cyclic AMP-treated cells. Using reverse transcription/PCR, we demonstrated that the chimeric transcripts were from the internal PEPCK promoter rather than a functional or reconstituted Moloney LTR. PEPCK-reporter chimeras delivered by retroviral vectors demonstrated a level of expression more consistent with the level of expression of the native PEPCK gene than did transfected chimeras. This expression system should prove useful for studies of the physiological modulation of gene expression in renal tissues.

Cytokine-induced release of endothelin-1 from porcine renal epithelial cell line

1990 ◽  
Vol 169 (2) ◽  
pp. 578-584 ◽  
Author(s):  
Kazuki Ohta ◽  
Yukio Hirata ◽  
Taihei Imai ◽  
Kazuo Kanno ◽  
Toshiaki Emori ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Renal Epithelial Cell ◽  
Endothelin 1 ◽  
Renal Epithelial Cell Line

NMR STUDIES ON A RENAL EPITHELIAL CELL LINE: LLC-PK1/CI 4

1987 ◽  
pp. 115-120
Author(s):  
A. W. H. Jans ◽  
E. Kellenbach ◽  
J. Luiq ◽  
P. Raniewski ◽  
B. Griewel ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Nmr Studies ◽  
Renal Epithelial Cell ◽  
Renal Epithelial Cell Line

Modulation of energy status and cytotoxicity induced by FK506 and cyclosporin A in a renal epithelial cell line

1997 ◽  
Vol 71 (8) ◽  
pp. 529-531 ◽  
Author(s):  
France Massicot ◽  
Chantal Martin ◽  
Hélène Dutertre-Catella ◽  
Sophie Ellouk-Achard ◽  
Chuong Pham-Huy ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cyclosporin A ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Energy Status ◽  
Renal Epithelial Cell ◽  
Renal Epithelial Cell Line
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