Mitochondrial ATAD3A regulates milk biosynthesis and proliferation of mammary epithelial cells from dairy cow via the mTOR pathway

2018 ◽  
Vol 42 (5) ◽  
pp. 533-542 ◽  
Author(s):  
Dongying Chen ◽  
Xiaohan Yuan ◽  
Lijie Liu ◽  
Minghui Zhang ◽  
Bo Qu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Dairy Cow ◽  
Mammary Epithelial Cells ◽  
Mtor Pathway ◽  
Mammary Epithelial

14-3-3γ affects mTOR pathway and regulates lactogenesis in dairy cow mammary epithelial cells

2015 ◽  
Vol 51 (7) ◽  
pp. 697-704 ◽  
Author(s):  
Nagam Khudhair ◽  
Chaochao Luo ◽  
Ahmed Khalid ◽  
Li Zhang ◽  
Shuang Zhang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Dairy Cow ◽  
Mammary Epithelial Cells ◽  
Mtor Pathway ◽  
Mammary Epithelial

Liver X receptor α participates in LPS-induced reduction of triglyceride synthesis in bovine mammary epithelial cells

2020 ◽  
pp. 1-7
Author(s):  
Jianfa Wang ◽  
Shuai Lian ◽  
Jun Song ◽  
Hai Wang ◽  
Xu Zhang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Dairy Cow ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Liver X Receptor ◽  
Mammary Epithelial ◽  
Triglyceride Synthesis ◽  
Milk Fat Depression ◽  
Liver X Receptor Α

Abstract Lipopolysaccharides (LPS) could induce milk fat depression via regulating the body and blood fat metabolism. However, it is not completely clear how LPS might regulate triglyceride synthesis in dairy cow mammary epithelial cells (DCMECs). DCMECs were isolated and purified from dairy cow mammary tissue and treated with LPS. The level of triglyceride synthesis, the expression and activity of the liver X receptor α (LXRα), enzymes related to de novo fatty acid synthesis, and the expression of the fatty acid transporters were investigated. We found that LPS decreased the level of triglyceride synthesis via a down-regulation of the transcription, translation, and nuclear translocation level of the LXRα. The results also indicated that the transcription level of the LXRα target genes, sterol regulatory element binding protein 1 (SREBP1), fatty acid synthetase (FAS), acetyl-CoA carboxylase-1 (ACC1), were significantly down-regulated in DCMECs after LPS treatment. Our data may provide new insight into the mechanisms of milk fat depression caused by LPS.

Septin6 regulates cell growth and casein synthesis in dairy cow mammary epithelial cells via mTORC1 pathway

2019 ◽  
Vol 86 (2) ◽  
pp. 181-187
Author(s):  
Bin Li ◽  
Zhuzha Basang ◽  
Lijun Hu ◽  
Liu Liu ◽  
Nan Jiang
Keyword(s):  
Cell Growth ◽  
Epithelial Cells ◽  
Dairy Cow ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Regulatory Factor ◽  
Mtor Inhibition ◽  
Mtorc1 Pathway ◽  
Positive Factor ◽  
Casein Synthesis

AbstractThis research paper addresses the hypothesis that Septin6 is a key regulatory factor influencing amino acid (AA)-mediated cell growth and casein synthesis in dairy cow mammary epithelial cells (DCMECs). DCMECs were treated with absence of AA (AA−), restricted concentrations of AA (AAr) or normal concentrations of AA (AA+) for 24 h. Cell growth, expression of CSN2 and Septin6 were increased in response to AA supply. Overexpressing or inhibiting Septin6 demonstrated that cell growth, expression of CSN2, mTOR, p-mTOR, S6K1 and p-S6K1 were up-regulated by Septin6. Furthermore, overexpressing or inhibiting mTOR demonstrated that the increase in cell growth and expression of CSN2 in response to Septin6 overexpression were inhibited by mTOR inhibition, and vice versa. Our hypothesis was supported; we were able to show that Septin6 is an important positive factor for cell growth and casein synthesis, it up-regulates AA-mediated cell growth and casein synthesis through activating mTORC1 pathway in DCMECs.

scholarly journals Effects of glucose on lactose synthesis in mammary epithelial cells from dairy cow

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Ye Lin ◽  
Xiaoxu Sun ◽  
Xiaoming Hou ◽  
Bo Qu ◽  
Xuejun Gao ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Dairy Cow ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Lactose Synthesis

Vaccaria segetalisactivates cellular lactogenesis signaling pathway of dairy cow mammary epithelial cells in vitro

2015 ◽  
Vol 49 (2) ◽  
pp. 199
Author(s):  
L.I. Nan ◽  
Wei Cheng-jie ◽  
Meng Hai-yang ◽  
Sun Xiao-xu ◽  
Wang Li-na ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Signaling Pathway ◽  
Dairy Cow ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial

Comparative phosphoproteomics analysis of the effects of L-methionine on dairy cow mammary epithelial cells

2012 ◽  
Vol 92 (4) ◽  
pp. 433-442 ◽  
Author(s):  
Limin Lu ◽  
Xuejun Gao ◽  
Qingzhang Li ◽  
Jianguo Huang ◽  
Rong Liu ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Epithelial Cells ◽  
Dairy Cow ◽  
Milk Protein ◽  
Mammary Epithelial Cells ◽  
Reversed Phase ◽  
Trna Synthetase ◽  
Mammary Epithelial ◽  
Two Dimensional Gel Electrophoresis ◽  
Phosphorylated Proteins

Lu, L., Gao, X., Li, Q., Huang, J., Liu, R. and Li, H. 2012. Comparative phosphoproteomics analysis of the effects of L-methionine on dairy cow mammary epithelial cells. Can. J. Anim. Sci. 92: 433–442. L-methionine is an essential amino acid that plays fundamental roles in protein synthesis. Many nuclear phosphorylated proteins such as Stat5 (signal transducer and activator of transcription 5) and mTOR (mammalian target of rapamycin) regulate milk protein synthesis. But a comprehensive understanding of transcriptional and posttranscriptional regulation of milk protein synthesis is lacking. In the current study, two-dimensional gel electrophoresis (2-DE)/MS-based proteomics analysis was used to identify phosphoproteins responsible for milk protein synthesis in dairy cow mammary epithelial cells (DCMECs). The effects of L-methionine on DCMECs were analyzed by CASY (Counter Analyser System) technique, reversed phase high performance liquid chromatography. The results showed that rate of cell proliferation and expression of β-casein were increased in DCMECs treated with 0.6 mM L-methionine for 24 h. Five proteins for which expression was significantly increased in DCMECs were selected, and their expression changes were verified by quantitative real-time PCR and Western blot analysis. The five up-regulated expressed phosphoproteins included Staphylococcal nuclease domain-containing protein 1(SND1), Septin-6, Glycyl-tRNA synthetase (GARS), Twinfilin-1 and eukaryotic elongation factor1-beta (eEF1B). This study revealed that availability of L-methionine influences the levels of nuclear phosphorylated proteins of DCMECs which opens a new avenue for the study of the molecular mechanism linking to milk protein synthesis.

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