Simultaneous targeted inhibition of Sox2-Oct4 transcription factors using decoy oligodeoxynucleotides to repress stemness properties in mouse embryonic stem cells

2017 ◽  
Vol 41 (12) ◽  
pp. 1335-1344 ◽  
Author(s):  
Behrooz Johari ◽  
Jamil Zargan
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Targeted Inhibition

scholarly journals Regulation of Cyclin E by transcription factors of the naïve pluripotency network in mouse embryonic stem cells

Cell Cycle ◽  
2019 ◽  
Vol 18 (20) ◽  
pp. 2697-2712 ◽  
Author(s):  
Fabrice Gonnot ◽  
Diana Langer ◽  
Pierre-Yves Bourillot ◽  
Nathalie Doerflinger ◽  
Pierre Savatier
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Cyclin E ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

scholarly journals Directed Pancreatic Acinar Differentiation of Mouse Embryonic Stem Cells via Embryonic Signalling Molecules and Exocrine Transcription Factors

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54243 ◽  
Author(s):  
Fabien Delaspre ◽  
Mohammad Massumi ◽  
Marta Salido ◽  
Bernat Soria ◽  
Philippe Ravassard ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Signalling Molecules

PI3K/Akt-dependent functions of TFII-I transcription factors in mouse embryonic stem cells

2012 ◽  
Vol 113 (4) ◽  
pp. 1122-1131 ◽  
Author(s):  
Nyam-Osor Chimge ◽  
Aleksandr V. Makeyev ◽  
Sabine J. Waigel ◽  
Badam Enkhmandakh ◽  
Dashzeveg Bayarsaihan
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

scholarly journals Regulation of Cyclin E by transcription factors of the naïve pluripotency network in mouse embryonic stem cells

2019 ◽  
Author(s):  
Fabrice Gonnot ◽  
Diana Langer ◽  
Pierre-Yves Bourillot ◽  
Nathalie Doerflinger ◽  
Pierre Savatier
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Binding Sites ◽  
Cyclin E ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Negative Regulator ◽  
Expression Level ◽  
Luciferase Assay

AbstractContinuous, non-cell cycle-dependent expression of cyclin E is a characteristic feature of mouse embryonic stem cells (ESCs). We studied the 5’ regulatory region of Cyclin E, also known as Ccne1, and identified binding sites for transcription factors of the naïve pluripotency network, including Esrrb, Klf4, and Tfcp2l1 within 1 kilobase upstream of the transcription start site. Luciferase assay and chromatin immunoprecipitation-quantitative polymerase chain reaction (ChiP–qPCR) study highlighted one binding site for Esrrb that is essential to transcriptional activity of the promoter region, and three binding sites for Klf4 and Tfcp2l1. Knockdown of Esrrb, Klf4, and Tfcp2l1 reduced Cyclin E expression whereas overexpression of Esrrb and Klf4 increased it, indicating a strong correlation between the expression level of these factors and that of cyclin E. We observed that cyclin E overexpression delays differentiation induced by Esrrb depletion, suggesting that cyclin E is an important target of Esrrb for differentiation blockade. We observed that mESCs express a low level of miR-15a and that transfection of a miR-15a mimic decreases Cyclin E mRNA level. These results lead to the conclusion that the high expression level of Cyclin E in mESCs can be attributed to transcriptional activation by Esrrb as well as to the absence of its negative regulator, miR-15a.

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