Untargeted metabolomics analysis of adipogenic transformation in OP9-DL1 cells using liquid chromatography-mass spectrometry: Implications for thymic adipogenesis

2017 ◽  
Vol 41 (4) ◽  
pp. 447-456 ◽  
Author(s):  
Jianxin Tan ◽  
Yajun Wang ◽  
Siliang Wang ◽  
Nannan Zhang ◽  
Simeng Wu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Untargeted Metabolomics ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Metabolomics Analysis

scholarly journals Integrating comprehensive two-dimensional gas chromatography mass spectrometry and parallel two-dimensional liquid chromatography mass spectrometry for untargeted metabolomics

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4331-4341 ◽  
Author(s):  
Md Aminul Islam Prodhan ◽  
Biyun Shi ◽  
Ming Song ◽  
Liqing He ◽  
Fang Yuan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Untargeted Metabolomics ◽  
Gas Chromatography Mass Spectrometry ◽  
Two Dimensional ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

The diverse characteristics and large number of entities make metabolite separation challenging in metabolomics.

Strategies for large-scale targeted metabolomics quantification by liquid chromatography-mass spectrometry

The Analyst ◽  
2016 ◽  
Vol 141 (23) ◽  
pp. 6362-6373 ◽  
Author(s):  
Juntuo Zhou ◽  
Yuxin Yin
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Large Scale ◽  
Liquid Chromatography Mass Spectrometry ◽  
Targeted Metabolomics ◽  
Chromatography Mass Spectrometry ◽  
Great Progress ◽  
Analytical Strategies ◽  
Metabolomics Analysis

Advances in liquid chromatography-mass spectrometry (LC-MS) instruments and analytical strategies have brought about great progress in targeted metabolomics analysis.

scholarly journals DEVELOPMENT AND APPLICATION OF A TEST MIXTURE FOR UNTARGETED LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF URINE SAMPLES

Química Nova ◽  
2021 ◽  
Author(s):  
Clarisse Torres ◽  
Vinicius Sardela ◽  
Fernanda Scalco ◽  
Francisco Aquino ◽  
Rafael Garrett
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Source ◽  
Urine Samples ◽  
Untargeted Metabolomics ◽  
Mass Spectrometry Analysis ◽  
Quality Data ◽  
Liquid Chromatography Mass Spectrometry ◽  
Test Mixture ◽  
Chromatography Mass Spectrometry

Metabolic profiling of complex biological matrices based on liquid chromatography-mass spectrometry (LC-MS) allows detecting a wide range of metabolites with distinct concentrations and physicochemical properties. Given the complexity of samples and the necessity of a comprehensive approach in untargeted metabolomics, quality control strategies are mandatory to obtain high-quality data. The LC-MS performance must be monitored and evaluated to guarantee data reliability. In this study, a test mixture (TM) was developed, systematically evaluated, and applied to untargeted metabolomics of urine samples from individuals suspected of inborn errors of metabolism. The TM was composed of fifteen analytes that eluted across the entire gradient in reversed-phase columns and ionized in positive/negative electrospray modes. It helped set the LC-MS conditions for urine analysis, from sample reconstitution solvent to selecting the MS ion source parameters. The TM quickly indicated column stationary phase degradation during the batch analysis when employed to monitor and evaluate the LC-MS system in an untargeted metabolomic analysis. Thus, in addition to pooled QC samples, a TM can be employed in untargeted metabolomics to rapidly assess the system performance avoiding unnecessary efforts for further data treatment and multivariate analysis of poor-quality data

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