The matricellular protein CCN1 regulates TNF-α induced vascular endothelial cell apoptosis

2015 ◽  
Vol 40 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Jin Zhang ◽  
Gongxiong Wu ◽  
Haibin Dai
Keyword(s):  
Endothelial Cell ◽  
Cell Apoptosis ◽  
Vascular Endothelial Cell ◽  
Matricellular Protein ◽  
Vascular Endothelial ◽  
Endothelial Cell Apoptosis ◽  
Tnf Α

Role of protein kinase C β2 activation in TNF-α-induced human vascular endothelial cell apoptosis

2009 ◽  
Vol 87 (3) ◽  
pp. 221-229 ◽  
Author(s):  
Fang Wang ◽  
Hui-min Liu ◽  
Michael G. Irwin ◽  
Zhong-yuan Xia ◽  
Zhiyong Huang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Apoptosis ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Endothelial Cell Apoptosis ◽  
Human Vascular Endothelial Cell ◽  
Kinase C ◽  
Tnf Α

The circulatory inflammatory cytokine tumor necrosis factor alpha (TNF-α) is increased in pathologic conditions that initiate or exacerbate vascular endothelial injury, such as diabetes. Protein kinase C (PKC) has been shown to play a critical role in TNF-α-induced human endothelial cell apoptosis. However, the relative roles played by specific isoforms of PKC in TNF-α-induced human endothelial cell apoptosis have not been addressed. We investigated the effects of a selective PKCβ2 inhibitor (CGP53353) on TNF-α-induced apoptosis in human vascular endothelial cells (cell line ECV304) and on the production of reactive oxygen species and nitric oxide, and compared its effects with rottlerin, a reagent that has been shown to reduce PKCδ protein levels. Cultured human vascular endothelial cells (ECV304) were treated for 24 h with one of 4 regimes: 40 ng/mL TNF-α alone (TNF-α), TNF-α with 10 µmol/L rottlerin (T+rottlerin), TNF-α with 1 µmol/L CGP53353 (T+CGP), or untreated (control). Cell viability was measured by MTT assay, and cell apoptosis was assessed by flow cytometry. TNF-α-induced endothelial cell apoptosis was associated with dramatic increases in production of intracellular hydrogen peroxide (approximately 20 times greater than control) and superoxide (approximately 16 times greater than control), as measured by dichlorofluorescein and dihydroethidium fluorescent staining, respectively. This increase was accompanied by reduced activity of superoxide dismutase and glutathione peroxidase and, subsequently, an increase in the lipid peroxidation product malondialdehyde. CGP53353, but not rottlerin, abolished or attenuated all these changes. We conclude that PKCβ2 plays a major role in TNF-α-induced human vascular endothelial cell apoptosis.

scholarly journals Uric acid promotes oxidative stress and enhances vascular endothelial cell apoptosis in rats with middle cerebral artery occlusion

2018 ◽  
Vol 38 (3) ◽  
Author(s):  
Chengfu Song ◽  
Xiangdong Zhao
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Endothelial Cell ◽  
Cell Apoptosis ◽  
Vascular Endothelial Cell ◽  
Artery Occlusion ◽  
Vascular Endothelial ◽  
Endothelial Cell Apoptosis ◽  
Related Factors ◽  
Cerebral Artery Occlusion

In patients with cerebral infarction (CI), elevated serum uric acid (UA) level may exacerbate the occurrence and development of carotid atherosclerosis (AS). Our study intended to explore the underlying mechanism. We enrolled 86 patients with CI, and divided them into four groups: Non-AS, AS-mild, AS-moderate, and AS-severe groups; the levels of UA and oxidative stress-related factors in serum were detected. The middle cerebral artery occlusion (MCAO) model was used to stimulate CI in rats, and different doses of UA were administrated. The levels of oxidative stress-related factors in serum were detected. Hematoxylin & eosin (H&E) staining was used to observe the morphological alterations, and the apoptotic cell death detection kit was used to detect apoptotic cells. Increased UA concentration and enhanced oxidative stress were found in AS patients. H&E staining results showed that UA treatment exacerbated morphological damage in rats with MCAO, promoted oxidative stress, and enhanced vascular endothelial cell apoptosis in rats with MCAO.

Crocetin prevents AGEs-induced vascular endothelial cell apoptosis

2006 ◽  
Vol 54 (4) ◽  
pp. 268-274 ◽  
Author(s):  
Min Xiang ◽  
Min Yang ◽  
Chenghua Zhou ◽  
Juan Liu ◽  
Wenna Li ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Apoptosis ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Endothelial Cell Apoptosis

scholarly journals Pericardial fluid from patients with unstable angina induces vascular endothelial cell apoptosis

2000 ◽  
Vol 35 (7) ◽  
pp. 1785-1790 ◽  
Author(s):  
Atsushi Iwakura ◽  
Masatoshi Fujita ◽  
Koji Hasegawa ◽  
Tatsuya Sawamura ◽  
Ryuji Nohara ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Unstable Angina ◽  
Cell Apoptosis ◽  
Vascular Endothelial Cell ◽  
Pericardial Fluid ◽  
Vascular Endothelial ◽  
Endothelial Cell Apoptosis
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