High-level expression and purification of soluble bioactive recombinant human heparin-binding epidermal growth factor inEscherichia coli

2015 ◽  
Vol 39 (7) ◽  
pp. 858-864 ◽  
Author(s):  
Mostafa Khalili ◽  
Mohammad Reza Soleyman ◽  
Maryam Baazm ◽  
Cordian Beyer
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Inescherichia Coli ◽  
Heparin Binding ◽  
Expression And Purification ◽  
High Level Expression ◽  
Epidermal Growth ◽  
High Level

scholarly journals Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rasoul Kowsar ◽  
Shahrzad Ronasi ◽  
Nima Sadeghi ◽  
Khaled Sadeghi ◽  
Akio Miyamoto
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Dna Fragmentation ◽  
Acrosome Reaction ◽  
Developmental Competence ◽  
Ros Production ◽  
Sperm Capacitation ◽  
Bovine Sperm ◽  
Epidermal Growth ◽  
High Level

AbstractUpon insemination, sperm cells are exposed to components of the female reproductive tract (FRT) fluids, such as urea and epidermal growth factor (EGF). It has been shown that both urea and EGF use EGF receptor signaling and produce reactive oxygen species (ROS) that are required at certain levels for sperm capacitation and acrosome reaction. We therefore hypothesized that during bovine sperm capacitation, a high level of urea and EGF could interfere with sperm function through overproduction of ROS. High-level urea (40 mg/dl urea is equal to 18.8 mg/dl of blood urea nitrogen) significantly increased ROS production and TUNEL-positive sperm (sperm DNA fragmentation, sDF) percentage, but decreased HOS test score, progressive motility, acrosome reaction and capacitation. The EGF reversed the negative effects of urea on all sperm parameters, with the exception of ROS production and DNA fragmentation, which were higher in urea-EGF-incubated sperm than in control-sperm. The developmental competence of oocytes inseminated with urea-EGF-incubated sperm was significantly reduced compared to the control. A close association of ROS production or sDF with 0-pronuclear and sperm non-capacitation rates was found in the network analysis. In conclusion, EGF enhanced urea-reduced sperm motility; however, it failed to reduce urea-increased sperm ROS or sDF levels and to enhance subsequent oocyte competence. The data suggests that any study to improve sperm quality should be followed by a follow-up assessment of the fertilization outcome.

scholarly journals Heparin-binding epidermal growth factor-like growth factor is an autocrine growth factor for human keratinocytes.

1994 ◽  
Vol 269 (31) ◽  
pp. 20060-20066 ◽  
Author(s):  
K. Hashimoto ◽  
S. Higashiyama ◽  
H. Asada ◽  
E. Hashimura ◽  
T. Kobayashi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Human Keratinocytes ◽  
Heparin Binding ◽  
Autocrine Growth ◽  
Autocrine Growth Factor ◽  
Epidermal Growth

scholarly journals Differential Pathways of Angiotensin II-Induced Extracellularly Regulated Kinase 1/2 Phosphorylation in Specific Cell Types: Role of Heparin-Binding Epidermal Growth Factor

2004 ◽  
Vol 18 (8) ◽  
pp. 2035-2048 ◽  
Author(s):  
Bukhtiar H. Shah ◽  
Akin Yesilkaya ◽  
J. Alberto Olivares-Reyes ◽  
Hung-Dar Chen ◽  
László Hunyady ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Types ◽  
Specific Cell ◽  
Heparin Binding ◽  
Epidermal Growth
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