Polyamines cause elevation of steroid 5α-reductase mRNA levels by suppressing mRNA degradation in C6 glioma cells

2014 ◽  
Vol 38 (10) ◽  
pp. 1132-1137 ◽  
Author(s):  
Kyoji Morita ◽  
Mi-Sook Lee ◽  
Song Her ◽  
Naoyoshi Nishibori
Keyword(s):  
Glioma Cells ◽  
Mrna Degradation ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Mrna Levels ◽  
Reductase Mrna

Influence of serum-free culture conditions on steroid 5α-reductase mRNA expression in rat C6 glioma cells

1999 ◽  
Vol 830 (1) ◽  
pp. 179-182 ◽  
Author(s):  
Kyoji Morita ◽  
Yoshihiro Tsuruo ◽  
Kazunori Ishimura ◽  
Song Her ◽  
Rose Ann Bell ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Glioma Cells ◽  
Culture Conditions ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Serum Free ◽  
Reductase Mrna ◽  
Rat C6 Glioma

Regulation of cyclooxygenase 2 mRNA degradation by rosiglitazone in C6 glioma cells in the presence of inflammation inductors

2015 ◽  
Vol 9 (4) ◽  
pp. 337-341
Author(s):  
E. V. Pankevich ◽  
D. V. Chistyakov ◽  
A. A. Astakhova ◽  
O. S. Strelkova ◽  
M. G. Sergeeva
Keyword(s):  
Glioma Cells ◽  
Mrna Degradation ◽  
C6 Glioma ◽  
Cyclooxygenase 2 ◽  
C6 Glioma Cells

Trichostatin A Enhances Glutamate Transporter GLT-1 mRNA Levels in C6 Glioma Cells via Neurosteroid-Mediated Cell Differentiation

2012 ◽  
Vol 49 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Mari Itoh ◽  
Takara Hiroi ◽  
Naoyoshi Nishibori ◽  
Takefumi Sagara ◽  
Song Her ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Trichostatin A ◽  
Glutamate Transporter ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Mrna Levels

Radiosensitivity of glioma to Gamma Knife treatment enhanced in vitro and in vivo by RNA interfering Ku70 that is mediated by a recombinant adenovirus

2010 ◽  
Vol 113 (Special_Supplement) ◽  
pp. 228-235 ◽  
Author(s):  
Qiang Jia ◽  
Yanhe Li ◽  
Desheng Xu ◽  
Zhenjiang Li ◽  
Zhiyuan Zhang ◽  
...  
Keyword(s):  
Western Blot ◽  
Gamma Knife ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
C6 Cells

Object The authors sought to evaluate modification of the radiation response of C6 glioma cells in vitro and in vivo by inhibiting the expression of Ku70. To do so they investigated the effect of gene transfer involving a recombinant replication-defective adenovirus containing Ku70 short hairpin RNA (Ad-Ku70shRNA) combined with Gamma Knife treatment (GKT). Methods First, Ad-Ku70shRNA was transfected into C6 glioma cells and the expression of Ku70 was measured using Western blot analysis. In vitro, phenotypical changes in C6 cells, including proliferation, cell cycle modification, invasion ability, and apoptosis were evaluated using the MTT (3′(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) assay, Western blot analysis, and cell flow cytometry. In vivo, parental C6 cells transfected with Ad-Ku70shRNA were implanted stereotactically into the right caudate nucleus in Sprague-Dawley rats. After GKS, apoptosis was analyzed using the TUNEL (terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling) method. The inhibitory effects on growth and invasion that were induced by expression of proliferating cell nuclear antigen and matrix metalloproteinase–9 were determined using immunohistochemical analyses. Results The expression of Ku70 was clearly inhibited in C6 cells after transfection with Ad-Ku70shRNA. In vitro following transfection, the C6 cells showed improved responses to GKT, including suppression of proliferation and invasion as well as an increased apoptosis index. In vivo following transfection of Ad-Ku70shRNA, the therapeutic efficacy of GKT in rats with C6 gliomas was greatly enhanced and survival times in these animals were prolonged. Conclusions Our data support the potential for downregulation of Ku70 expression in enhancing the radiosensitivity of gliomas. The findings of our study indicate that targeted gene therapy–mediated inactivation of Ku70 may represent a promising strategy in improving the radioresponsiveness of gliomas to GKT.

scholarly journals Transfected Go1 alpha inhibits the calcium dependence of beta-adrenergic stimulated cAMP accumulation in C6 glioma cells.

1993 ◽  
Vol 268 (12) ◽  
pp. 8980-8989
Author(s):  
N. Charpentier ◽  
L. Prézeau ◽  
J. Carrette ◽  
R. Bertorelli ◽  
G. Le Cam ◽  
...  
Keyword(s):  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Camp Accumulation ◽  
Beta Adrenergic ◽  
Calcium Dependence
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