scholarly journals Simultaneous reduction of MAD2 and BUBR1 expression induces mitotic spindle alterations associated with p53 dependent cell cycle arrest and death

2014 ◽  
Vol 38 (8) ◽  
pp. 933-941 ◽  
Author(s):  
Laura Lentini ◽  
Desirèe Piscitello ◽  
Lorena Veneziano ◽  
Aldo Di Leonardo
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Mitotic Spindle ◽  
Simultaneous Reduction ◽  
Cycle Arrest ◽  
Dependent Cell

scholarly journals MdmX Protects p53 from Mdm2-Mediated Degradation

2000 ◽  
Vol 20 (3) ◽  
pp. 1001-1007 ◽  
Author(s):  
Mark W. Jackson ◽  
Steven J. Berberich
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Nuclear Export ◽  
P53 Protein ◽  
Ring Finger ◽  
Ring Finger Domain ◽  
Cycle Arrest ◽  
Dependent Cell ◽  
Potential Basis ◽  
P53 Tumor Suppressor Protein

ABSTRACT The p53 tumor suppressor protein is stabilized in response to cellular stress, resulting in activation of genes responsible for either cell cycle arrest or apoptosis. The cellular pathway for releasing normal cells from p53-dependent cell cycle arrest involves the Mdm2 protein. Recently, a p53-binding protein with homology to Mdm2 was identified and called MdmX. Like Mdm2, MdmX is able to bind p53 and inhibit p53 transactivation; however, the ability of MdmX to degrade p53 has yet to be examined. We report here that MdmX is capable of associating with p53 yet is unable to facilitate nuclear export or induce p53 degradation. In addition, expression of MdmX can reverse Mdm2-targeted degradation of p53 while maintaining suppression of p53 transactivation. Using a series of MdmX deletions, we have determined that there are two distinct domains of the MdmX protein that can stabilize p53 in the presence of Mdm2. One domain requires MdmX interaction with p53 and results in the retention of both proteins within the nucleus and repression of p53 transactivation. The second domain involves the MdmX ring finger and results in stabilization of p53 and an increase in p53 transactivation. The potential basis for stabilization and increased p53 transactivation by the MdmX ring finger domain is discussed. Based on these observations, we propose that the MdmX protein may function to maintain a nuclear pool of p53 protein in undamaged cells.

scholarly journals Enforced Expression of p14ARF Induces p53-Dependent Cell Cycle Arrest but Not Apoptosis

Cell Cycle ◽  
2005 ◽  
Vol 4 (3) ◽  
pp. 465-472 ◽  
Author(s):  
Stuart Gallagher ◽  
Richard F. Kefford ◽  
Helen Rizos
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cycle Arrest ◽  
Dependent Cell

scholarly journals Aberrant Expression of Nucleostemin Activates p53 and Induces Cell Cycle Arrest via Inhibition of MDM2

2008 ◽  
Vol 28 (13) ◽  
pp. 4365-4376 ◽  
Author(s):  
Mu-Shui Dai ◽  
Xiao-Xin Sun ◽  
Hua Lu
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Cycle Arrest ◽  
Ribosomal Proteins ◽  
Cell Cycle Checkpoint ◽  
Aberrant Expression ◽  
Cycle Arrest ◽  
Reduce Cell Proliferation ◽  
Cycle Checkpoint ◽  
Dependent Cell

ABSTRACT The nucleolar protein nucleostemin (NS) is essential for cell proliferation and early embryogenesis. Both depletion and overexpression of NS reduce cell proliferation. However, the mechanisms underlying this regulation are still unclear. Here, we show that NS regulates p53 activity through the inhibition of MDM2. NS binds to the central acidic domain of MDM2 and inhibits MDM2-mediated p53 ubiquitylation and degradation. Consequently, ectopic overexpression of NS activates p53, induces G1 cell cycle arrest, and inhibits cell proliferation. Interestingly, the knockdown of NS by small interfering RNA also activates p53 and induces G1 arrest. These effects require the ribosomal proteins L5 and L11, since the depletion of NS enhanced their interactions with MDM2 and the knockdown of L5 or L11 abrogated the NS depletion-induced p53 activation and cell cycle arrest. These results suggest that a p53-dependent cell cycle checkpoint monitors changes of cellular NS levels via the impediment of MDM2 function.

Integrin-linked kinase regulates p38 MAPK-dependent cell cycle arrest in ureteric bud development

2010 ◽  
Vol 123 (19) ◽  
pp. e1-e1
Author(s):  
J. Smeeton ◽  
X. Zhang ◽  
N. Bulus ◽  
G. Mernaugh ◽  
A. Lange ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
P38 Mapk ◽  
Ureteric Bud ◽  
Bud Development ◽  
Cycle Arrest ◽  
Dependent Cell ◽  
Integrin Linked Kinase

An unstructured model of metabolic and temperature dependent cell cycle arrest in hybridoma batch and fed-batch cultures

2015 ◽  
Vol 93 ◽  
pp. 260-273 ◽  
Author(s):  
D.G. García Münzer ◽  
M. Ivarsson ◽  
C. Usaku ◽  
T. Habicher ◽  
M. Soos ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Fed Batch ◽  
Temperature Dependent ◽  
Unstructured Model ◽  
Batch Cultures ◽  
Cycle Arrest ◽  
Dependent Cell
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