Myocardial structural protein expression in umbilical cord blood mesenchymal stem cells after myogenic induction

2013 ◽  
Vol 37 (9) ◽  
pp. 899-904 ◽  
Author(s):  
Nan Ma ◽  
Fangbao Ding ◽  
Junwen Zhang ◽  
Chunrong Bao ◽  
Hong Zhong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Protein Expression ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Structural Protein ◽  
Myogenic Induction

scholarly journals Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Contribute to Chondrogenesis in Coculture with Chondrocytes

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Xingfu Li ◽  
Li Duan ◽  
Yujie Liang ◽  
Weimin Zhu ◽  
Jianyi Xiong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Protein Expression ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Type I ◽  
Low Density

Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have been shown as the most potential stem cell source for articular cartilage repair. In this study, we aimed to develop a method for long-term coculture of human articular chondrocytes (hACs) and hUCB-MSCs at low density in vitro to determine if the low density of hACs could enhance the hUCB-MSC chondrogenic differentiation as well as to determine the optimal ratio of the two cell types. Also, we compared the difference between direct coculture and indirect coculture at low density. Monolayer cultures of hUCB-MSCs and hACs were investigated at different ratios, at direct cell-cell contact groups for 21 days. Compared to direct coculture, hUCB-MSCs and hACs indirect contact culture significantly increased type II collagen (COL2) and decreased type I collagen (COL1) protein expression levels. SRY-box 9 (SOX9) mRNA levels and protein expression were highest in indirect coculture. Overall, these results indicate that low density direct coculture induces fibrocartilage. However, indirect coculture in conditioned chondrocyte cell culture medium can increase expression of chondrogenic markers and induce hUCB-MSCs differentiation into mature chondrocytes. This work demonstrates that it is possible to promote chondrogenesis of hUCB-MSCs in combination with hACs, further supporting the concept of novel coculture strategies for tissue engineering.

scholarly journals Characterization and clinical application of mesenchymal stem cells from equine umbilical cord blood

2013 ◽  
Vol 14 (3) ◽  
pp. 367 ◽  
Author(s):  
Jun-Gu Kang ◽  
Sang-Bum Park ◽  
Min-Soo Seo ◽  
Hyung-Sik Kim ◽  
Joon-Seok Chae ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Clinical Application ◽  
Umbilical Cord Blood

scholarly journals Prospects for the therapeutic development of umbilical cord blood-derived mesenchymal stem cells

2020 ◽  
Vol 12 (12) ◽  
pp. 1511-1528
Author(s):  
Soyoun Um ◽  
Jueun Ha ◽  
Soo Jin Choi ◽  
Wonil Oh ◽  
Hye Jin Jin
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Therapeutic Development

scholarly journals Gene Therapy of Intracranial Glioma Using Interleukin 12–Secreting Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells

2011 ◽  
Vol 22 (6) ◽  
pp. 733-743 ◽  
Author(s):  
Chung Heon Ryu ◽  
Sang-Hoon Park ◽  
Soon A Park ◽  
Seong Muk Kim ◽  
Jung Yeon Lim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Interleukin 12 ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord
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