Higher propensity of Wharton's jelly derived mesenchymal stromal cells towards neuronal lineage in comparison to those derived from adipose and bone marrow

2013 ◽  
Vol 37 (5) ◽  
pp. 507-515 ◽  
Author(s):  
Sudha Balasubramanian ◽  
Charan Thej ◽  
Parvathy Venugopal ◽  
Nancy Priya ◽  
Zubaidah Zakaria ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Neuronal Lineage

Comparison of chemokine and receptor gene expression between Wharton's jelly and bone marrow-derived mesenchymal stromal cells

Cytotherapy ◽  
2012 ◽  
Vol 14 (1) ◽  
pp. 26-33 ◽  
Author(s):  
Sudha Balasubramanian ◽  
Parvathy Venugopal ◽  
Swathi Sundarraj ◽  
Zubaidah Zakaria ◽  
Anish Sen Majumdar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Receptor Gene ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Receptor Gene Expression

scholarly journals Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton's jelly and bone marrow

FEBS Open Bio ◽  
2016 ◽  
Vol 6 (11) ◽  
pp. 1054-1066 ◽  
Author(s):  
Claire Mennan ◽  
Sharon Brown ◽  
Helen McCarthy ◽  
Eleni Mavrogonatou ◽  
Dimitris Kletsas ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Wharton's Jelly

scholarly journals Direct Comparison of Wharton's Jelly and Bone Marrow-Derived Mesenchymal Stromal Cells to Enhance Engraftment of Cord Blood CD34+Transplants

2015 ◽  
Vol 24 (22) ◽  
pp. 2649-2659 ◽  
Author(s):  
Mark van der Garde ◽  
Melissa van Pel ◽  
Jose Eduardo Millán Rivero ◽  
Alice de Graaf-Dijkstra ◽  
Manon C. Slot ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cord Blood ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Cord Blood Cd34

scholarly journals Characterization of Bone Marrow and Wharton’s Jelly Mesenchymal Stromal Cells Response on Multilayer Braided Silk and Silk/PLCL Scaffolds for Ligament Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2163
Author(s):  
Xing Liu ◽  
Adrien Baldit ◽  
Emilie de Brosses ◽  
Frédéric Velard ◽  
Ghislaine Cauchois ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Composite Scaffold ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Collagen Iii ◽  
Silk Scaffold ◽  
Ligament Tissue Engineering

(1) Background: A suitable scaffold with adapted mechanical and biological properties for ligament tissue engineering is still missing. (2) Methods: Different scaffold configurations were characterized in terms of morphology and a mechanical response, and their interactions with two types of stem cells (Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) and bone marrow mesenchymal stromal cells (BM-MSCs)) were assessed. The scaffold configurations consisted of multilayer braids with various number of silk layers (n = 1, 2, 3), and a novel composite scaffold made of a layer of copoly(lactic acid-co-(e-caprolactone)) (PLCL) embedded between two layers of silk. (3) Results: The insertion of a PLCL layer resulted in a higher porosity and better mechanical behavior compared with pure silk scaffold. The metabolic activities of both WJ-MSCs and BM-MSCs increased from day 1 to day 7 except for the three-layer silk scaffold (S3), probably due to its lower porosity. Collagen I (Col I), collagen III (Col III) and tenascin-c (TNC) were expressed by both MSCs on all scaffolds, and expression of Col I was higher than Col III and TNC. (4) Conclusions: the silk/PLCL composite scaffolds constituted the most suitable tested configuration to support MSCs migration, proliferation and tissue synthesis towards ligament tissue engineering.

scholarly journals Interaction between Macrophages and Human Mesenchymal Stromal Cells Derived from Bone Marrow and Wharton’s Jelly—A Comparative Study

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1822
Author(s):  
Marta Dymowska ◽  
Aleksandra Aksamit ◽  
Katarzyna Zielniok ◽  
Monika Kniotek ◽  
Beata Kaleta ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Basic Research ◽  
Wharton’S Jelly ◽  
Clinical Settings ◽  
Flow Cytometry Analysis ◽  
Scratch Assay ◽  
Wharton's Jelly ◽  
Well Assay

Despite intensive clinical research on the use of mesenchymal stromal cells (MSCs), further basic research in this field is still required. Herein, we compared human bone marrow MSCs (BM-MSCs, n = 6) and Wharton’s jelly MSCs (WJ-MSCs, n = 6) in their ability to interact with human primary macrophages. Evaluation of secretory potential revealed that under pro-inflammatory stimulation, WJ-MSCs secreted significantly more IL-6 than BM-MSCs (2-fold). This difference did not translate into the effect of MSCs on macrophages: both types of MSCs significantly directed M1-like macrophages toward the M2 phenotype (based on CD206 expression) to a similar extent. This observation was consistent both in flow cytometry analysis and immunocytochemical assessment. The effect of MSCs on macrophages was sustained when IL-6 signaling was blocked with Tocilizumab. Macrophages, regardless of polarization status, enhanced chemotaxis of both BM-MSCs and WJ-MSCs (p < 0.01; trans-well assay), with WJ-MSCs being significantly more responsive to M1-derived chemotactic signals than BM-MSCs. Furthermore, WJ-MSCs increased their motility (scratch assay) when exposed to macrophage-conditioned medium while BM-MSCs did not. These results indicate that although both BM-MSCs and WJ-MSCs have the ability to reciprocally interact with macrophages, the source of MSCs could slightly but significantly modify the response under clinical settings.

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