Enhanced maintenance of rat islets of Langerhans on laminin-coated electrospun nanofibrillar matrix in vitro

2013 ◽  
Vol 37 (4) ◽  
pp. 370/a-379/a ◽  
Author(s):  
Mozhdeh Sojoodi ◽  
Ali Farrokhi ◽  
Azadeh Moradmand ◽  
Hossein Baharvand
Keyword(s):  
Islets Of Langerhans ◽  
Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Nanofibrillar Matrix

scholarly journals Pentitols and insulin release by isolated rat islets of Langerhans

1968 ◽  
Vol 109 (3) ◽  
pp. 333-339 ◽  
Author(s):  
W Montague ◽  
K W Taylor
Keyword(s):  
Insulin Release ◽  
Islets Of Langerhans ◽  
Pentose Phosphate ◽  
Rat Islets ◽  
Isolated Islets Of Langerhans ◽  
Isolated Rat Islets ◽  
Rat Islets Of Langerhans ◽  
Stimulate Insulin Release ◽  
Isolated Rat

1. Insulin secretion was studied in isolated islets of Langerhans obtained by collagenase digestion of rat pancreas. In addition to responding to glucose and mannose as do whole pancreas and pancreas slices in vitro, isolated rat islets also secrete insulin in response to xylitol, ribitol and ribose, but not to sorbitol, mannitol, arabitol, xylose or arabinose. 2. Xylitol and ribitol readily reduce NAD+ when added to a preparation of ultrasonically treated islets. 3. Adrenaline (1μm) inhibits the effects of glucose and xylitol on insulin release. Mannoheptulose and 2-deoxy-glucose, however, inhibit the response to glucose but not that to xylitol. 4. The intracellular concentration of glucose 6-phosphate is increased when islets are incubated with glucose but not with xylitol, suggesting that xylitol does not promote insulin release by conversion into glucose 6-phosphate. 5. Theophylline (5mm) potentiates the effect of 20mm-glucose on insulin release from isolated rat islets of Langerhans, but has no effect on xylitol-mediated release. These results indicate that xylitol does not stimulate insulin release by alterations in the intracellular concentrations of cyclic AMP. 6. A possible role for the metabolism of hexoses via the pentose phosphate pathway in the stimulation of insulin release is discussed.

Stimulation of insulin secretion from isolated rat islets of Langerhans by melittin

1984 ◽  
Vol 4 (8) ◽  
pp. 665-671 ◽  
Author(s):  
Noel G. Morgan ◽  
William Montague
Keyword(s):  
Insulin Secretion ◽  
Islets Of Langerhans ◽  
Maximal Response ◽  
Secretory Rate ◽  
Rat Islets ◽  
Cell Plasma ◽  
Rat Islets Of Langerhans ◽  
Dose Dependent ◽  
Stimulation Of

Melittin, an amphipathic polypeptide, stimulated the secretion of insulin from rat islets of Langerhans incubated in vitro. The secretory response was dose-dependent and saturable with half the maximal response elicited by a melittin concentration of 4 μg/ml. The response was rapid in onset, an increase in secretion occurring within 2 rain of exposure of the islets to melittin (2 μg/ml). An enhanced secretory rate could be maintained for at least 40 rain in the presence of melittin but declined steadily when the agent was removed. Stimulation of secretion by melittin occurred in the absence of glucose and in the presence of both 4 mM and 8 mM glucose but not in the presence of 20 mM glucose. The effect of melittin on secretion was dependent on the presence of extracellular calcium but was not inhibited by norepinephrine. The data suggest that melittin may be a valuable agent for further study of the role played by the B-cell plasma membrane in the regulation of insulin secretion.

Opioid peptides in rat islets of Langerhans. Immunoreactive met- and leu-enkephalins and BAM-22P

Diabetes ◽  
1986 ◽  
Vol 35 (1) ◽  
pp. 52-57 ◽  
Author(s):  
K. I. Timmers ◽  
N. R. Voyles ◽  
C. King ◽  
M. Wells ◽  
R. Fairtile ◽  
...  
Keyword(s):  
Islets Of Langerhans ◽  
Opioid Peptides ◽  
Rat Islets ◽  
Rat Islets Of Langerhans

Effects of epicatechin on rat islets of Langerhans

Diabetes ◽  
1984 ◽  
Vol 33 (3) ◽  
pp. 291-296 ◽  
Author(s):  
C. S. Hii ◽  
S. L. Howell
Keyword(s):  
Islets Of Langerhans ◽  
Rat Islets ◽  
Rat Islets Of Langerhans
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