Pigment epithelium-derived factor delays cellular senescence of human mesenchymal stem cells in vitro by reducing oxidative stress

2013 ◽  
Vol 37 (4) ◽  
pp. 305-313 ◽  
Author(s):  
Yukun Cao ◽  
Ting Yang ◽  
Chunhu Gu ◽  
Dinghua Yi
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cellular Senescence ◽  
Pigment Epithelium ◽  
Human Mesenchymal Stem Cells ◽  
Pigment Epithelium Derived Factor

scholarly journals Mesenchymal stem cells overexpressing PEDF decrease the angiogenesis of gliomas

2013 ◽  
Vol 33 (2) ◽  
Author(s):  
Qiaoshu Wang ◽  
Zhaoyun Zhang ◽  
Tianling Ding ◽  
Zi Chen ◽  
Tao Zhang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Brain Tumour ◽  
Pigment Epithelium ◽  
Human Mesenchymal Stem Cells ◽  
Migration Assay ◽  
Migratory Activity ◽  
Promising Therapeutic Approach ◽  
Novel Strategy

The present study is an exploration of a novel strategy to target a therapeutic gene to brain tumour tissues. In the present study, we evaluated the feasibility of using hMSCs (human mesenchymal stem cells) to deliver PEDF (pigment epithelium-derived factor), a potent inhibitor of tumour angiogenesis, in a model of intracranial gliomas. To assess its potential of tracking gliomas, MSCs (mesenchymal stem cells) were injected into the cerebral hemisphere and it showed that MSCs infiltrated into the vessel beds and scattered throughout the tumour. In vitro migration assay showed that the VEGF (vascular endothelial growth factor) enhanced MSC migration. In contrast, the migratory activity of MSCs was significantly inhibited with the presence of PEDF. Systematic delivery of AAV (adeno-associated virus)–PEDF to established glioma xenografts resulted in increased apoptosis of gliomas. In addition, MSC–PEDF treatment prolonged the survival of mice bearing U87 gliomas. Taken together, these data validate that MSCs–PEDF can migrate and deliver PEDF to target glioma cells, which may be a novel and promising therapeutic approach for refractory brain tumour.

In Vitro Assessment of Hydroxyapatite Coating on the Surface of Additive Manufactured Ti6Al4V Scaffolds

2016 ◽  
Vol 879 ◽  
pp. 2444-2449 ◽  
Author(s):  
Ekaterina Chudinova ◽  
Maria Surmeneva ◽  
Andrey Koptioug ◽  
Irina V. Savintseva ◽  
Irina Selezneva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Nanocrystalline Structure ◽  
X Ray Diffraction ◽  
Ha Coating ◽  
Magnetron Sputter Deposition ◽  
In Vitro Assessment ◽  
Average Size

Custom orthopedic and dental implants may be fabricated by additive manufacturing (AM), for example using electron beam melting technology. This study is focused on the modification of the surface of Ti6Al4V alloy coin-like scaffolds fabricated via AM technology (EBM®) by radio frequency (RF) magnetron sputter deposition of hydroxyapatite (HA) coating. The scaffolds with HA coating were characterized by Scanning Electron microscopy, X-ray diffraction. HA coating showed a nanocrystalline structure with the crystallites of an average size of 32±9 nm. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells was studied using biological short-term tests in vitro. In according to in vitro assessment, thin HA coating stimulated the attachment and proliferation of cells. Human mesenchymal stem cells cultured on the HA-coated scaffold also formed mineralized nodules.

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