scholarly journals Rapid estimation of membrane protein orientation in liposomes

ChemBioChem ◽  
2021 ◽  
Author(s):  
Sabina Deutschmann ◽  
Lukas Rimle ◽  
Christoph von Ballmoos
Keyword(s):  
Membrane Protein ◽  
Protein Orientation ◽  
Rapid Estimation

Prediction of membrane protein orientation in lipid bilayers: a theoretical approach

2001 ◽  
Vol 20 (3) ◽  
pp. 235-244 ◽  
Author(s):  
Frederic Basyn ◽  
Benoit Charloteaux ◽  
Annick Thomas ◽  
Robert Brasseur
Keyword(s):  
Membrane Protein ◽  
Lipid Bilayers ◽  
Theoretical Approach ◽  
Protein Orientation

Peptide Disc Mediated Control of Membrane Protein Orientation in Supported Lipid Bilayers for Surface-Sensitive Investigations

2019 ◽  
Vol 92 (1) ◽  
pp. 1081-1088 ◽  
Author(s):  
Alessandra Luchini ◽  
Frederik Grønbæk Tidemand ◽  
Nicolai Tidemand Johansen ◽  
Mario Campana ◽  
Javier Sotres ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Lipid Bilayers ◽  
Supported Lipid Bilayers ◽  
Protein Orientation

scholarly journals Detailed Analysis of Protein Topology of Extracellular Vesicles–Evidence of Unconventional Membrane Protein Orientation

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Aleksander Cvjetkovic ◽  
Su Chul Jang ◽  
Barbora Konečná ◽  
Johanna L. Höög ◽  
Carina Sihlbom ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Detailed Analysis ◽  
Extracellular Vesicles ◽  
Protein Orientation ◽  
Protein Topology

scholarly journals Probing membrane protein orientation and structure using fast magic-angle-spinning solid-state NMR

2004 ◽  
Vol 30 (3) ◽  
pp. 253-265 ◽  
Author(s):  
O. C. Andronesi ◽  
J. R. Pfeifer ◽  
L. Al-Momani ◽  
S. Özdirekcan ◽  
D. T. S. Rijkers ◽  
...  
Keyword(s):  
Solid State ◽  
Membrane Protein ◽  
Solid State Nmr ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Protein Orientation ◽  
Angle Spinning ◽  
Fast Magic Angle Spinning

TMCC3 localizes at the three-way junctions for the proper tubular network of the endoplasmic reticulum

2019 ◽  
Vol 476 (21) ◽  
pp. 3241-3260
Author(s):  
Sindhu Wisesa ◽  
Yasunori Yamamoto ◽  
Toshiaki Sakisaka
Keyword(s):  
Endoplasmic Reticulum ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Mammalian Cells ◽  
Coiled Coil ◽  
Transmembrane Domains ◽  
Domain Family ◽  
Coiled Coil Domain ◽  
U2os Cells ◽  
Er Membrane

The tubular network of the endoplasmic reticulum (ER) is formed by connecting ER tubules through three-way junctions. Two classes of the conserved ER membrane proteins, atlastins and lunapark, have been shown to reside at the three-way junctions so far and be involved in the generation and stabilization of the three-way junctions. In this study, we report TMCC3 (transmembrane and coiled-coil domain family 3), a member of the TEX28 family, as another ER membrane protein that resides at the three-way junctions in mammalian cells. When the TEX28 family members were transfected into U2OS cells, TMCC3 specifically localized at the three-way junctions in the peripheral ER. TMCC3 bound to atlastins through the C-terminal transmembrane domains. A TMCC3 mutant lacking the N-terminal coiled-coil domain abolished localization to the three-way junctions, suggesting that TMCC3 localized independently of binding to atlastins. TMCC3 knockdown caused a decrease in the number of three-way junctions and expansion of ER sheets, leading to a reduction of the tubular ER network in U2OS cells. The TMCC3 knockdown phenotype was partially rescued by the overexpression of atlastin-2, suggesting that TMCC3 knockdown would decrease the activity of atlastins. These results indicate that TMCC3 localizes at the three-way junctions for the proper tubular ER network.

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