scholarly journals Engineered Human Induced Pluripotent Cells Enable Genetic Code Expansion in Brain Organoids

ChemBioChem ◽  
2021 ◽  
Author(s):  
Lea S. Husen ◽  
Anna‐Maria Katsori ◽  
Birthe Meineke ◽  
Lars O. Tjernberg ◽  
Sophia Schedin‐Weiss ◽  
...  
Keyword(s):  
Genetic Code ◽  
Pluripotent Cells ◽  
Induced Pluripotent Cells ◽  
Genetic Code Expansion ◽  
Induced Pluripotent ◽  
Human Induced Pluripotent Cells

scholarly journals Engineered human induced pluripotent cells enable genetic code expansion in brain organoids

ChemBioChem ◽  
2021 ◽  
Author(s):  
Lea S. van Husen ◽  
Anna-Maria Katasori ◽  
Birthe Meineke ◽  
Lars O. Tjernberg ◽  
Sophia Schedin-Weiss ◽  
...  
Keyword(s):  
Genetic Code ◽  
Pluripotent Cells ◽  
Induced Pluripotent Cells ◽  
Genetic Code Expansion ◽  
Induced Pluripotent ◽  
Human Induced Pluripotent Cells

scholarly journals Engineered human induced pluripotent cells enable genetic code expansion in brain organoids

2021 ◽  
Author(s):  
Lea van Husen ◽  
Anna Maria Katsori ◽  
Birthe Meineke ◽  
Lars O Tjernberg ◽  
Sophia Schedin-Weiss ◽  
...  
Keyword(s):  
Genetic Code ◽  
Fluorescent Dyes ◽  
Induced Pluripotent Stem Cell ◽  
Cell Types ◽  
Trna Synthetase ◽  
Induced Pluripotent Cells ◽  
Wide Range ◽  
Induced Pluripotent ◽  
Human Induced Pluripotent Cells ◽  
Tissue Models

Human induced pluripotent stem cell (hiPSC) technology has revolutionized human biology. A wide range of cell types and tissue models can be derived from hiPSCs to study complex human diseases. Here, we use PiggyBac mediated transgenesis to engineer hiPSCs with an expanded genetic code. We demonstrate that genomic integration of expression cassettes for a pyrrolysyl-tRNA synthetase (PylRS), pyrrolysyl-tRNA (PylT) and the target protein of interest enables site-specific incorporation of a non-canonical amino acid (ncAA) in response to amber stop codons. Neural stem cells, neurons and brain organoids derived from the engineered hiPSCs continue to express the amber suppression machinery and produce ncAA-bearing reporter. The incorporated ncAA can serve as a minimal bioorthogonal handle for further modifications by labeling with fluorescent dyes. Site-directed ncAA mutagenesis will open a wide range of applications to probe and manipulate proteins in brain organoids and other hiPSC-derived cell types and complex tissue models.

scholarly journals Generation of human induced pluripotent cells on a human feeder layer

2009 ◽  
Vol 18 ◽  
pp. S-7-S-8
Author(s):  
M Dijon-Grinand ◽  
S Assou ◽  
L Nadal ◽  
C Monzo ◽  
S Hamamah ◽  
...  
Keyword(s):  
Feeder Layer ◽  
Pluripotent Cells ◽  
Induced Pluripotent Cells ◽  
Induced Pluripotent ◽  
Human Induced Pluripotent Cells

scholarly journals Aberrant α-Adrenergic Hypertrophic Response in Cardiomyocytes from Human Induced Pluripotent Cells

2014 ◽  
Vol 3 (5) ◽  
pp. 905-914 ◽  
Author(s):  
Gabor Földes ◽  
Elena Matsa ◽  
János Kriston-Vizi ◽  
Thomas Leja ◽  
Stefan Amisten ◽  
...  
Keyword(s):  
Pluripotent Cells ◽  
Hypertrophic Response ◽  
Induced Pluripotent Cells ◽  
Induced Pluripotent ◽  
Human Induced Pluripotent Cells

Scalable culture of human induced pluripotent cells on microcarriers under xeno-free conditions using single-use vertical-wheel™ bioreactors

2018 ◽  
Vol 93 (12) ◽  
pp. 3597-3606 ◽  
Author(s):  
Carlos AV Rodrigues ◽  
Teresa P Silva ◽  
Diogo ES Nogueira ◽  
Tiago G Fernandes ◽  
Yas Hashimura ◽  
...  
Keyword(s):  
Pluripotent Cells ◽  
Induced Pluripotent Cells ◽  
Single Use ◽  
Induced Pluripotent ◽  
Human Induced Pluripotent Cells

scholarly journals Human induced pluripotent cells in personalized treatment of monogenic epilepsies

2020 ◽  
Author(s):  
Nazanin A. Mohammadi ◽  
Kristine Freude ◽  
Henriette Haukedal ◽  
Zeynep Tümer ◽  
Rikke S. Møller
Keyword(s):  
Personalized Treatment ◽  
Pluripotent Cells ◽  
Induced Pluripotent Cells ◽  
Induced Pluripotent ◽  
Human Induced Pluripotent Cells
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