scholarly journals A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection

ChemBioChem ◽  
2020 ◽  
Vol 21 (18) ◽  
pp. 2696-2700
Author(s):  
Annabel Kitowski ◽  
Gonçalo J. L. Bernardes
Keyword(s):  
Plasmodium Infection ◽  
Metabolic Oligosaccharide Engineering

scholarly journals A Sweet Galactose Transfer – Metabolic Oligosaccharide Engineering as a Tool to Study Glycans in Plasmodium Infection

2019 ◽  
Author(s):  
Annabel Kitowski ◽  
Gonçalo Bernardes
Keyword(s):  
Plasmodium Infection ◽  
Liver Stage ◽  
Hepatic Cells ◽  
Metabolic Oligosaccharide Engineering

The use of artificial galactose derivatives and labelling through iEDDA reaction in the liver stage of <i>Plasmodium </i>infection showed increased uptake in infected hepatic cells, through participation of GLUT1 transporters. Furthermore, unprotected derivatives are transferred from the mosquito host to the <i>Plasmodium</i> <i>berghei</i> parasite. This strategy has the potential to provide new insights into <i>Plasmodium</i> glycobiology.

scholarly journals A Sweet Galactose Transfer – Metabolic Oligosaccharide Engineering as a Tool to Study Glycans in Plasmodium Infection

2019 ◽  
Author(s):  
Annabel Kitowski ◽  
Gonçalo Bernardes
Keyword(s):  
Plasmodium Infection ◽  
Liver Stage ◽  
Hepatic Cells ◽  
Metabolic Oligosaccharide Engineering

The use of artificial galactose derivatives and labelling through iEDDA reaction in the liver stage of <i>Plasmodium </i>infection showed increased uptake in infected hepatic cells, through participation of GLUT1 transporters. Furthermore, unprotected derivatives are transferred from the mosquito host to the <i>Plasmodium</i> <i>berghei</i> parasite. This strategy has the potential to provide new insights into <i>Plasmodium</i> glycobiology.

Asymptomatic low-density Plasmodium infection during non-transmission season: a community-based cross-sectional study in two districts of North Eastern Region, India

2021 ◽  
Author(s):  
Hari Shankar ◽  
Sobhan Phookan ◽  
Mrigendra Pal Singh ◽  
Ram Suresh Bharti ◽  
Naseem Ahmed ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Diagnostic Tools ◽  
Eastern Region ◽  
Low Density ◽  
Plasmodium Infection ◽  
Sectional Study ◽  
Cross Sectional ◽  
Health Agencies ◽  
North Eastern ◽  
Asymptomatic Infections

Abstract Background Malaria elimination requires targeting asymptomatic and low-density Plasmodium infections that largely remain undetected. Therefore we conducted a cross-sectional study to estimate the burden of asymptomatic and low-density Plasmodium infection using conventional and molecular diagnostics. Methods A total of 9118 participants, irrespective of age and sex, were screened for malaria using rapid diagnostic tests (RDTs), microscopy and polymerase chain reaction. Results Among the participants, 707 presented with symptoms and 8411 without symptoms, of which Plasmodium was present in 15.6% (110/707) and 8.1% (681/8411), respectively. Low-density infection was found in 5.1% (145/2818) of participants and 8327 of 9118 were Plasmodium negative. Endemicity was propotional to asymptomatic infections (high endemicity 11.1% [404/3633] vs low endemicity 5.8% [277/4778]; odds ratio [OR] 2.0 [95% confidence interval {CI} 1.7 to 2.4]) but inversely related to low-density infection (high endemicity 3.7% [57/1545] vs low endemicity 6.9% [88/1273]; OR 1.9 [95% CI 1.4 to 2.7]). The spleen rate in children 2–9 y of age was 17.9% (602/3368) and the enlarged spleen index was 1.6. Children between 8 and 14 y showed higher odds for asymptomatic (adjusted OR [aOR] 1.75 [95% CI 1.4 to 2.2]) and low-density infections (aOR 0.63 [95% CI 0.4 to 1.0)] than adults. Conclusions The prevalence of asymptomatic and low-density Plasmodium infection undermines the usefulness of standard diagnostic tools used by health agencies. This necessitates deploying molecular tools in areas where malaria microscopy/RDTs indicate a dearth of infection.

scholarly journals The fibrinolytic system enables the onset of Plasmodium infection in the mosquito vector and the mammalian host

2021 ◽  
Vol 7 (6) ◽  
pp. eabe3362 ◽  
Author(s):  
Thiago Luiz Alves e Silva ◽  
Andrea Radtke ◽  
Amanda Balaban ◽  
Tales Vicari Pascini ◽  
Zarna Rajeshkumar Pala ◽  
...  
Keyword(s):  
Plasminogen Activators ◽  
Fibrinolytic System ◽  
Matrix Proteins ◽  
Parasite Development ◽  
Mammalian Host ◽  
Mosquito Vector ◽  
Plasminogen Activation ◽  
Plasmodium Infection ◽  
Human Plasminogen ◽  
Vertebrate Hosts

Plasmodium parasites must migrate across proteinaceous matrices to infect the mosquito and vertebrate hosts. Plasmin, a mammalian serine protease, degrades extracellular matrix proteins allowing cell migration through tissues. We report that Plasmodium gametes recruit human plasminogen to their surface where it is processed into plasmin by corecruited plasminogen activators. Inhibition of plasminogen activation arrests parasite development early during sexual reproduction, before ookinete formation. We show that increased fibrinogen and fibrin in the blood bolus, which are natural substrates of plasmin, inversely correlate with parasite infectivity of the mosquito. Furthermore, we show that sporozoites, the parasite form transmitted by the mosquito to humans, also bind plasminogen and plasminogen activators on their surface, where plasminogen is activated into plasmin. Surface-bound plasmin promotes sporozoite transmission by facilitating parasite migration across the extracellular matrices of the dermis and of the liver. The fibrinolytic system is a potential target to hamper Plasmodium transmission.

Parasite and Host Erythrocyte Kinomics of Plasmodium Infection

2021 ◽  
Author(s):  
Jack Adderley ◽  
Tayla Williamson ◽  
Christian Doerig
Keyword(s):  
Plasmodium Infection

scholarly journals Energy metabolism affects susceptibility of Anopheles gambiae mosquitoes to Plasmodium infection

2011 ◽  
Vol 41 (6) ◽  
pp. 349-355 ◽  
Author(s):  
Jose Henrique M. Oliveira ◽  
Renata L.S. Gonçalves ◽  
Giselle A. Oliveira ◽  
Pedro L. Oliveira ◽  
Marcus F. Oliveira ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Anopheles Gambiae ◽  
Plasmodium Infection

scholarly journals Monitoring of Plasmodium infection in humans and potential vectors of malaria in a newly emerged focus in southern Iran

2017 ◽  
Vol 111 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Mohsen Kalantari ◽  
Zahra Soltani ◽  
Mostafa Ebrahimi ◽  
Masoud Yousefi ◽  
Masoumeh Amin ◽  
...  
Keyword(s):  
Plasmodium Infection ◽  
Southern Iran
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