In Vitro Selection of New DNA Aptamers for Human Vascular Endothelial Growth Factor 165

ChemBioChem ◽  
2020 ◽  
Vol 21 (14) ◽  
pp. 2029-2036 ◽  
Author(s):  
Sepehr Manochehry ◽  
Jimmy Gu ◽  
Erin M. McConnell ◽  
Bruno J. Salena ◽  
Yingfu Li
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
In Vitro Selection ◽  
Dna Aptamers ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Selection Of

“All-in-one” in vitro selection of collagen-binding vascular endothelial growth factor

Biomaterials ◽  
2018 ◽  
Vol 161 ◽  
pp. 270-278 ◽  
Author(s):  
Shin-Hye Park ◽  
Takanori Uzawa ◽  
Fumiyuki Hattori ◽  
Shuichi Ogino ◽  
Naoki Morimoto ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
In Vitro Selection ◽  
Vascular Endothelial ◽  
Collagen Binding ◽  
Endothelial Growth Factor ◽  
Selection Of

scholarly journals Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro

2021 ◽  
Author(s):  
Kamil Wartalski ◽  
Gabriela Gorczyca ◽  
Jerzy Wiater ◽  
Zbigniew Tabarowski ◽  
Małgorzata Duda
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Primary Component ◽  
Marker Genes ◽  
Vascular Endothelial ◽  
Ovarian Cortex ◽  
Endothelial Growth Factor

AbstractEndothelial cells (ECs), the primary component of the vasculature, play a crucial role in neovascularization. However, the number of endogenous ECs is inadequate for both experimental purposes and clinical applications. Porcine ovarian putative stem cells (poPSCs), although not pluripotent, are characterized by great plasticity. Therefore, this study aimed to investigate whether poPSCs have the potential to differentiate into cells of endothelial lineage. poPSCs were immunomagnetically isolated from postnatal pig ovaries based on the presence of SSEA-4 protein. Expression of mesenchymal stem cells (MSCs) markers after pre-culture, both at the level of mRNA: ITGB1, THY, and ENG and corresponding protein: CD29, CD90, and CD105 were significantly higher compared to the control ovarian cortex cells. To differentiate poPSCs into ECs, inducing medium containing vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF), epidermal growth factor (EGF), ascorbic acid, and heparin was applied. After 14 days, poPSC differentiation into ECs was confirmed by immunofluorescence staining for vascular endothelial cadherin (VECad) and vascular endothelial growth factor receptor-2 (VEGFR-2). Semi-quantitative WB analysis of these proteins confirmed their high abundance. Additionally, qRT-PCR showed that mRNA expression of corresponding marker genes: CDH5, KDR was significantly higher compared with undifferentiated poPSCs. Finally, EC functional status was confirmed by the migration test that revealed that they were capable of positive chemotaxis, while tube formation assay demonstrated their ability to develop capillary networks. In conclusion, our results provided evidence that poPSCs may constitute the MSC population in the ovary and confirmed that they might be a potential source of ECs for tissue engineering.

Evaluation of vascular endothelial growth factor addition in vitro on mouse inner ear progenitor cell cultures

Neuroreport ◽  
2006 ◽  
Vol 17 (13) ◽  
pp. 1369-1373 ◽  
Author(s):  
Juergen-Theodor Fraenzer ◽  
Frank-Peter Wachs ◽  
Otto Gleich ◽  
Anna-Katharina Licht ◽  
J??rgen Strutz
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Inner Ear ◽  
Cell Cultures ◽  
Progenitor Cell ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

Bacteria-induced release of white cell- and platelet-derived vascular endothelial growth factor in vitro

Vox Sanguinis ◽  
2001 ◽  
Vol 80 (3) ◽  
pp. 170-178 ◽  
Author(s):  
H. J. Nielsen ◽  
K. Werther ◽  
T. Mynster ◽  
M. N. Svendsen ◽  
S. Rosendahl ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
White Cell ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor
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