Design and Expeditious Synthesis of Quinoline‐Pyrene‐Based Ratiometric Fluorescent Probes for Targeting Lysosomal pH

ChemBioChem ◽  
2020 ◽  
Vol 21 (10) ◽  
pp. 1492-1498
Author(s):  
Pankaj E. Hande ◽  
Manjari Mishra ◽  
Fariyad Ali ◽  
Shobhna Kapoor ◽  
Anindya Datta ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Lysosomal Ph

pH-activatable near-infrared fluorescent probes for detection of lysosomal pH inside living cells

2014 ◽  
Vol 2 (28) ◽  
pp. 4500-4508 ◽  
Author(s):  
Giri K. Vegesna ◽  
Jagadeesh Janjanam ◽  
Jianheng Bi ◽  
Fen-Tair Luo ◽  
Jingtuo Zhang ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Living Cells ◽  
Lysosomal Ph

Four near-infrared fluorescent probes have been synthesized, characterized, and evaluated for detection of lysosomal pH inside living cells.

scholarly journals Role of lysosomal and cytosolic pH in the regulation of macrophage lysosomal enzyme secretion

1990 ◽  
Vol 272 (2) ◽  
pp. 407-414 ◽  
Author(s):  
H Tapper ◽  
R Sundler
Keyword(s):  
Intracellular Ph ◽  
Fluorescent Probes ◽  
Lysosomal Enzyme ◽  
Enzyme Secretion ◽  
Relative Role ◽  
Ion Composition ◽  
Cytosolic Ph ◽  
Lysosomal Ph ◽  
Cytosolic Acidification

Rapid and parallel secretion of lysosomal beta-N-acetylglucosaminidase and preloaded fluorescein-labelled dextran was initiated in macrophages by agents affecting intracellular pH (methylamine, chlorpromazine, and the ionophores monensin and nigericin). In order to evaluate the relative role of changes in lysosomal and cytosolic pH, these parameters were monitored by using pH-sensitive fluorescent probes [fluorescein-labelled dextran or 2′,7′-bis(carboxyethyl)-5(6)-carboxyfluorescein]. All agents except chlorpromazine caused large increases in lysosomal pH under conditions where they induced secretion. By varying extracellular pH and ion composition, the changes in lysosomal and cytosolic pH could be dissociated. Secretion was then found to be significantly modulated by changes in cytosolic pH, being enhanced by alkalinization and severely inhibited by cytosolic acidification. However, changes in cytosolic pH in the absence of stimulus were unable to initiate secretion. Dissociation of the effects on lysosomal and cytosolic pH was also achieved by combining stimuli with either nigericin or acetate. Further support for a role of intracellular pH in the control of lysosomal enzyme secretion was provided by experiments where bicarbonate was included in the medium. The present study demonstrates that an increase in lysosomal pH is sufficient to initiate lysosomal enzyme secretion in macrophages and provides evidence for a significant regulatory role of cytosolic pH.

Near-infrared fluorescent probes based on piperazine-functionalized BODIPY dyes for sensitive detection of lysosomal pH

2015 ◽  
Vol 3 (10) ◽  
pp. 2173-2184 ◽  
Author(s):  
Jingtuo Zhang ◽  
Mu Yang ◽  
Cong Li ◽  
Nethaniah Dorh ◽  
Fei Xie ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Living Cells ◽  
Sensitive Detection ◽  
Lysosomal Ph

Three BODIPY-based near-infrared fluorescent probes have been synthesized, characterized, and evaluated for the sensing and imaging of lysosomal pH inside living cells.

Lysosome-targeting ratiometric fluorescent pH probes based on long-wavelength BODIPY

2018 ◽  
Vol 6 (27) ◽  
pp. 4422-4426 ◽  
Author(s):  
Mengliang Zhu ◽  
Peipei Xing ◽  
Yabin Zhou ◽  
Lei Gong ◽  
Jinghui Zhang ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Living Cells ◽  
Long Wavelength ◽  
Lysosomal Ph ◽  
Acidic Conditions ◽  
Ph Probes

BODIPY-based ratiometric fluorescent probes show remarkable sensitivity under acidic conditions via a protonation-modulated ICT mechanism for monitoring lysosomal pH in living cells.

scholarly journals Near-infrared fluorescent probes with BODIPY donors and rhodamine and merocyanine acceptors for ratiometric determination of lysosomal pH variance

2019 ◽  
Vol 294 ◽  
pp. 1-13 ◽  
Author(s):  
Shuai Xia ◽  
Mingxi Fang ◽  
Jianbo Wang ◽  
Jianheng Bi ◽  
Wafa Mazi ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Lysosomal Ph
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