scholarly journals Deploying Fluorescent Nucleoside Analogues for High‐Throughput Inhibitor Screening

ChemBioChem ◽  
2019 ◽  
Vol 21 (1-2) ◽  
pp. 108-112 ◽  
Author(s):  
Leah Seebald ◽  
Amaël G. E. Madec ◽  
Barbara Imperiali
Keyword(s):  
High Throughput ◽  
Nucleoside Analogues ◽  
Inhibitor Screening ◽  
Fluorescent Nucleoside

scholarly journals Multiplex Enzyme Assays and Inhibitor Screening by Mass Spectrometry

2010 ◽  
Vol 15 (8) ◽  
pp. 1001-1007 ◽  
Author(s):  
Rakesh Rathore ◽  
Patrick Pribil ◽  
Jay J. Corr ◽  
William L. Seibel ◽  
Artem Evdokimov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Therapeutic Target ◽  
High Throughput Screening ◽  
Multiplex Assay ◽  
Enzyme Assays ◽  
Readout System ◽  
Inhibitor Screening ◽  
Serial Process ◽  
Pass Through

Current methods for high-throughput screening (HTS) use a serial process to evaluate compounds as inhibitors toward a single therapeutic target, but as the demand to reduce screening time and cost continues to grow, one solution is the development of multiplex technology. In this communication, the multiplex assay capability of a mass spectrometry (MS)–based readout system is verified using a kinase and esterase reaction simultaneously. Furthermore, the MS-based readout is shown to be compatible with a typical HTS workflow by identifying and validating several new inhibitors for each enzyme from a small library of compounds. These data confirm that it is possible to monitor inhibition of multiple therapeutic targets with one pass through the compound repository, thus demonstrating the potential for MS-based methods to become a method of choice for HTS of isolated enzymes.

High-throughput HDR inhibitor screening

2014 ◽  
Vol 57 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Hye Seon Kim ◽  
Bok-Kyu Shin ◽  
Jaehong Han
Keyword(s):  
High Throughput ◽  
Inhibitor Screening

scholarly journals Study of the Substrate-Binding Properties of Bovine Liver Adenosine Kinase and Inhibition by Fluorescent Nucleoside Analogues

1997 ◽  
Vol 248 (3) ◽  
pp. 930-937 ◽  
Author(s):  
Helene Pelicano ◽  
Georges Maury ◽  
Abdelaziz Elalaoui ◽  
Manijeh Shafiee ◽  
Jean-Louis Imbach ◽  
...  
Keyword(s):  
Substrate Binding ◽  
Bovine Liver ◽  
Adenosine Kinase ◽  
Nucleoside Analogues ◽  
Binding Properties ◽  
Fluorescent Nucleoside
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