scholarly journals Targetable Tetrazine‐Based Dynamic Nuclear Polarization Agents for Biological Systems

ChemBioChem ◽  
2020 ◽  
Vol 21 (9) ◽  
pp. 1315-1319 ◽  
Author(s):  
Byung Joon Lim ◽  
Bryce E. Ackermann ◽  
Galia T. Debelouchina
Keyword(s):  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Biological Systems

scholarly journals Targetable Tetrazine-Based Dynamic Nuclear Polarization Agents for Biological Systems

2019 ◽  
Author(s):  
Byung Joon Lim ◽  
Bryce E. Ackermann ◽  
Galia T. Debelouchina
Keyword(s):  
Dynamic Nuclear Polarization ◽  
Mammalian Cells ◽  
Nuclear Polarization ◽  
Biological Systems ◽  
Polarization Transfer ◽  
Unnatural Amino Acid ◽  
Great Promise ◽  
Cellular Environment ◽  
Transfer Mechanisms ◽  
Complex Polarization

AbstractDynamic nuclear polarization (DNP) has shown great promise as a tool to enhance the nuclear magnetic resonance (NMR) signals of proteins in the cellular environment. As the sensitivity increases, the ability to select and efficiently polarize a specific macromolecule over the cellular background has become desirable. Here, we address this need and present a tetrazine-based DNP polarization agent that can be targeted selectively to proteins containing the unnatural amino acid (UAA) norbornene-lysine. The UAA can be introduced efficiently by genetic means in the cellular milieu. Our approach is bio-orthogonal and easily adaptable to any protein of interest. We illustrate the scope of our methodology and investigate the DNP polarization transfer mechanisms in several biological systems. Our results present the first molecular view of the complex polarization transfer pathways in targeted DNP and ultimately pave the way to selective DNP-enhanced NMR spectroscopy in both bacterial and mammalian cells.

Porphyrins as Versatile, Aggregation-Tolerant, and Biocompatible Polarizing Agents for Triplet Dynamic Nuclear Polarization of Biomolecules

2021 ◽  
pp. 2645-2650
Author(s):  
Tomoyuki Hamachi ◽  
Koki Nishimura ◽  
Hironori Kouno ◽  
Yusuke Kawashima ◽  
Kenichiro Tateishi ◽  
...  
Keyword(s):  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization

Solvent-free dynamic nuclear polarization enhancements in organically modified mesoporous silica

2021 ◽  
Author(s):  
Marcos de Oliveira Jr. ◽  
Kevin Herr ◽  
Martin Brodrecht ◽  
Nadia Berenice Haro-Mares ◽  
Till Wissel ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Porous Materials ◽  
Structural Characterization ◽  
Dynamic Nuclear Polarization ◽  
High Field ◽  
Nuclear Polarization ◽  
Organically Modified ◽  
Field Dynamic ◽  
Free Dynamic

High-field Dynamic Nuclear Polarization is a powerful tool for the structural characterization of species on the surface of porous materials or nanoparticles. For these studies the main source of polarization...

scholarly journals Dynamic Nuclear Polarization of Silicon Carbide Micro- and Nanoparticles

2021 ◽  
Author(s):  
Min Lin ◽  
Vincent Breukels ◽  
Tom W. J. Scheenen ◽  
Jos M. J. Paulusse
Keyword(s):  
Silicon Carbide ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization

scholarly journals Dynamic Nuclear Polarization / solid-state NMR of membranes. Thermal effects and sample geometry

2019 ◽  
Vol 100 ◽  
pp. 70-76 ◽  
Author(s):  
Evgeniy Sergeevich Salnikov ◽  
Fabien Aussenac ◽  
Sebastian Abel ◽  
Armin Purea ◽  
Paul Tordo ◽  
...  
Keyword(s):  
Solid State ◽  
Dynamic Nuclear Polarization ◽  
Thermal Effects ◽  
Solid State Nmr ◽  
Nuclear Polarization ◽  
Sample Geometry

On The Potential of Dynamic Nuclear Polarization Enhanced Diamonds in Solid-State and Dissolution13C NMR Spectroscopy

ChemPhysChem ◽  
2016 ◽  
Vol 17 (17) ◽  
pp. 2691-2701 ◽  
Author(s):  
Christian O. Bretschneider ◽  
Ümit Akbey ◽  
Fabien Aussenac ◽  
Greg L. Olsen ◽  
Akiva Feintuch ◽  
...  
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization
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