scholarly journals Direct Enzymatic Synthesis of a Deep‐Blue Fluorescent Noncanonical Amino Acid from Azulene and Serine

ChemBioChem ◽  
2019 ◽  
Vol 21 (1-2) ◽  
pp. 80-83 ◽  
Author(s):  
Ella J. Watkins ◽  
Patrick J. Almhjell ◽  
Frances H. Arnold
Keyword(s):  
Amino Acid ◽  
Enzymatic Synthesis ◽  
Deep Blue ◽  
Noncanonical Amino Acid

Characterization of an isozyme of S-adenosylmethionine synthetase from rat liver

1984 ◽  
Vol 62 (5) ◽  
pp. 276-279 ◽  
Author(s):  
C. H. Lin ◽  
W. Chung ◽  
K. P. Strickland ◽  
A. J. Hudson
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Amino Acid ◽  
Enzymatic Synthesis ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Aromatic Amino Acids ◽  
Adenosylmethionine Synthetase ◽  
Cellulose Column

An isozyme of S-adenosylmethionine synthetase has been purified to homogeneity by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and gel filtration on a Sephadex G-200 column. The purified enzyme is very unstable and has a molecular weight of 120 000 consisting of two identical subunits. Amino acid analysis on the purified enzyme showed glycine, glutamate, and aspartate to be the most abundant and the aromatic amino acids to be the least abundant. It possesses tripolyphosphatase activity which can be stimulated five to six times by S-adenosylmethionine (20–40 μM). The findings support the conclusion that an enzyme-bound tripolyphosphate is an obligatory intermediate in the enzymatic synthesis of S-adenosylmethionine from ATP and methionine.

scholarly journals Programming Post-Translational Control over the Metabolic Labeling of Cellular Proteins with a Noncanonical Amino Acid

2017 ◽  
Vol 6 (8) ◽  
pp. 1572-1583 ◽  
Author(s):  
Emily E. Thomas ◽  
Naresh Pandey ◽  
Sarah Knudsen ◽  
Zachary T. Ball ◽  
Jonathan J. Silberg
Keyword(s):  
Amino Acid ◽  
Translational Control ◽  
Cellular Proteins ◽  
Metabolic Labeling ◽  
Noncanonical Amino Acid

scholarly journals Accurate Determination of Human CPR Conformational Equilibrium by smFRET Using Dual Orthogonal Noncanonical Amino Acid Labeling

ChemBioChem ◽  
2019 ◽  
Vol 20 (5) ◽  
pp. 659-666 ◽  
Author(s):  
Robert B. Quast ◽  
Fataneh Fatemi ◽  
Michel Kranendonk ◽  
Emmanuel Margeat ◽  
Gilles Truan
Keyword(s):  
Amino Acid ◽  
Conformational Equilibrium ◽  
Accurate Determination ◽  
Amino Acid Labeling ◽  
Noncanonical Amino Acid

scholarly journals A General Strategy for Engineering Noncanonical Amino Acid Dependent Bacterial Growth

2019 ◽  
Vol 141 (41) ◽  
pp. 16213-16216 ◽  
Author(s):  
Minseob Koh ◽  
Anzhi Yao ◽  
Patrick R. Gleason ◽  
Jeremy H. Mills ◽  
Peter G. Schultz
Keyword(s):  
Amino Acid ◽  
Bacterial Growth ◽  
General Strategy ◽  
Noncanonical Amino Acid

scholarly journals Engineering enzymes for noncanonical amino acid synthesis

2018 ◽  
Vol 47 (24) ◽  
pp. 8980-8997 ◽  
Author(s):  
Patrick J. Almhjell ◽  
Christina E. Boville ◽  
Frances H. Arnold
Keyword(s):  
Amino Acid ◽  
Protein Engineering ◽  
Case Studies ◽  
Acid Synthesis ◽  
Amino Acid Synthesis ◽  
Noncanonical Amino Acid

This tutorial review covers contemporary case studies of successful protein engineering to provide new noncanonical amino acid synthases, with emphasis on methods, outcomes, and mechanistic insights.

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