Evaluation of the Interaction between Bax and Hsp70 in Cells by Using a FRET System Consisting of a Fluorescent Amino Acid and YFP as a FRET Pair

ChemBioChem ◽  
2019 ◽  
Vol 21 (1-2) ◽  
pp. 59-63
Author(s):  
Seong‐Hyun Park ◽  
Wooseok Ko ◽  
Sang‐Hyun Park ◽  
Hyun Soo Lee ◽  
Injae Shin
Keyword(s):  
Amino Acid ◽  
Fluorescent Amino Acid ◽  
Fret Pair ◽  
In Cells

A study of cellular transport with the fluorescent amino acid, aminonaphthylalanine

1966 ◽  
Vol 116 ◽  
pp. 261-270 ◽  
Author(s):  
Sidney Udenfriend ◽  
Perola Zaltzman-Nirenberg ◽  
Gordon Guroff
Keyword(s):  
Amino Acid ◽  
Cellular Transport ◽  
Fluorescent Amino Acid

scholarly journals A genetically encoded fluorescent amino acid

2006 ◽  
Vol 103 (26) ◽  
pp. 9785-9789 ◽  
Author(s):  
D. Summerer ◽  
S. Chen ◽  
N. Wu ◽  
A. Deiters ◽  
J. W. Chin ◽  
...  
Keyword(s):  
Amino Acid ◽  
Fluorescent Amino Acid

Transport of L-tyrosine by B16/F10 melanoma cells: the effect of the intracellular content of other amino acids

1990 ◽  
Vol 97 (3) ◽  
pp. 479-485
Author(s):  
J.R. Jara ◽  
J.H. Martinez-Liarte ◽  
F. Solano ◽  
R. Penafiel
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Initial Rate ◽  
Aromatic Amino Acids ◽  
Melanoma Cells ◽  
Transport Systems ◽  
Specific Amino Acid ◽  
Intracellular Content ◽  
Tyrosine Hydroxylation ◽  
In Cells

The uptake of L-Tyr by B16/F10 malignant melanocytes in culture has been studied. These melanoma cells can either be depleted of amino acids by 1 h preincubation in Hanks' isotonic medium or preloaded with a specific amino acid by 1 h preincubation in the same solution containing 2 mM of the amino acid to be preloaded. By means of these pretreatments, it is shown that the rate of L-Tyr uptake is greatly dependent on the content of other amino acids inside the cells. The L-Tyr uptake is higher in cells preloaded with amino acids transported by the L and ASC systems than in cells depleted of amino acids or preloaded with amino acids transported by the A system. It is concluded that L-Tyr is mainly taken up by an exchange mechanism with other amino acids mediated by the L1 system, although the ASC system can also participate in the process. In agreement with that, the homo-exchange performed by cells preloaded with unlabelled L-Tyr is more efficient than any other hetero-exchange, although L-Dopa, the product of tyrosine hydroxylation in melanin synthesis, is almost as efficient as L-Tyr. Apart from aromatic amino acids, melanoma cells preloaded with L-Met and L-His also yield a high initial rate of L-Tyr uptake. The results herein suggest that melanoma cells do not have transport systems specific for L-Tyr, even if this amino acid is needed to carry out the differential pathway of this type of cells, melanosynthesis.

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