scholarly journals A Genetically Encoded Ratiometric pH Probe: Wavelength Regulation-Inspired Design of pH Indicators

ChemBioChem ◽  
2018 ◽  
Vol 19 (12) ◽  
pp. 1288-1295 ◽  
Author(s):  
Tetyana Berbasova ◽  
Setare Tahmasebi Nick ◽  
Meisam Nosrati ◽  
Zahra Nossoni ◽  
Elizabeth M. Santos ◽  
...  
Keyword(s):  
Ph Probe ◽  
Probe Wavelength ◽  
Ph Indicators ◽  
Wavelength Regulation

Fluorescence ratio imaging of dynamic intracellular ionic signals

1988 ◽  
Vol 46 ◽  
pp. 42-43
Author(s):  
R. Y. Tsien ◽  
A. Minta ◽  
M. Poenie ◽  
J.P.Y. Kao ◽  
A. Harootunian
Keyword(s):  
Binding Sites ◽  
Living Cells ◽  
Molecular Engineering ◽  
Ph Indicators ◽  
Highly Sensitive ◽  
Fluorescence Ratio Imaging ◽  
Ratio Imaging ◽  
Technical Advances ◽  
Indicator Dyes ◽  
Fluorescein Dyes

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca2+, H+, or Na+, or Mg2+. The Ca2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (Kd near 200 nM) and selectivity for Ca2+ to a versatile tetracarboxylate binding site3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pKa's and improve their retention inside cells. Na+ indicators are crown ethers with cavity sizes chosen to select Na+ over K+: Mg2+ indicators use tricarboxylate binding sites truncated from those of the Ca2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.

A nucleolus-targeting hill-type pH probe

2021 ◽  
Vol 335 ◽  
pp. 129712
Author(s):  
Yansheng Xiao ◽  
Yanchun Li ◽  
Fang Hu ◽  
Kangnan Wang ◽  
Zong-Wan Mao ◽  
...  
Keyword(s):  
Ph Probe

A Dual-Channel Hill-Type Small-Molecule pH Probe

2021 ◽  
Author(s):  
Fang Hu ◽  
Yunxia Huang ◽  
Yansheng Xiao ◽  
Yanchun Li ◽  
Xiao Luo ◽  
...  
Keyword(s):  
Small Molecule ◽  
Transition Width ◽  
Ph Probe ◽  
Dual Channel ◽  
Unique Approach

By combining a Hill-type pH probe and a pH-insensitive naphthalimide fluorophore, We synthesized a FRET-based ratiometric pH probe (PHHF), exhibiting a reduced pH transition width, representing a unique approach for...

A Single Fluorescent pH Probe for Simultaneous Two-Color Visualization of Nuclei and Mitochondria and Monitoring Cell Apoptosis

ACS Sensors ◽  
2021 ◽  
Author(s):  
Rui Yang ◽  
Xiuquan He ◽  
Guangle Niu ◽  
Fangfang Meng ◽  
Qing Lu ◽  
...  
Keyword(s):  
Cell Apoptosis ◽  
Ph Probe ◽  
Color Visualization

Reusable glass-bound pH indicators

1975 ◽  
Vol 47 (2) ◽  
pp. 348-351 ◽  
Author(s):  
G. Bruce. Harper
Keyword(s):  
Ph Indicators

A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out

2015 ◽  
Vol 51 (28) ◽  
pp. 6145-6148 ◽  
Author(s):  
Robert J. Meier ◽  
Johann M. B. Simbürger ◽  
Tero Soukka ◽  
Michael Schäferling
Keyword(s):  
Dynamic Range ◽  
Dual Wavelength ◽  
Luminescence Lifetime ◽  
Ph Sensing ◽  
Ph Probe ◽  
Broad Dynamic Range ◽  
Europium Chelate ◽  
Ratiometric Ph Sensing

A FRET system composed of a europium chelate and carboxynaphthofluorescein enables ratiometric pH sensing with an exceptionally broad dynamic range.

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