Re-exploration of the Codon Context Effect on Amber Codon-Guided Incorporation of Noncanonical Amino Acids inEscherichia coliby the Blue-White Screening Assay

ChemBioChem ◽  
2016 ◽  
Vol 17 (13) ◽  
pp. 1250-1256 ◽  
Author(s):  
Huan Xu ◽  
Yan Wang ◽  
Jiaqi Lu ◽  
Bo Zhang ◽  
Ziwei Zhang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Context Effect ◽  
Screening Assay ◽  
Noncanonical Amino Acids ◽  
Amber Codon ◽  
Codon Context

Noncanonical Amino Acids for Hypoxia-Responsive Peptide Self-Assembly and Fluorescence

2021 ◽  
Author(s):  
Binbin Hu ◽  
Na Song ◽  
Yawei Cao ◽  
Mingming Li ◽  
Xin Liu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Self Assembly ◽  
Noncanonical Amino Acids

scholarly journals Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons

2021 ◽  
Vol 16 (4) ◽  
pp. 766-774
Author(s):  
Jeffery M. Tharp ◽  
Oscar Vargas-Rodriguez ◽  
Alanna Schepartz ◽  
Dieter Söll
Keyword(s):  
Amino Acids ◽  
Noncanonical Amino Acids ◽  
Genetic Encoding ◽  
Nonsense Codons

scholarly journals A Genetically Encoded Picolyl Azide for Improved Live Cell Copper Click Labeling

2021 ◽  
Vol 9 ◽  
Author(s):  
Birthe Meineke ◽  
Johannes Heimgärtner ◽  
Alexander J. Craig ◽  
Michael Landreh ◽  
Lindon W. K. Moodie ◽  
...  
Keyword(s):  
Amino Acids ◽  
Mammalian Cells ◽  
Stop Codon ◽  
Bioorthogonal Chemistry ◽  
Bioorthogonal Reaction ◽  
Noncanonical Amino Acids ◽  
Selective Reactivity ◽  
Direct Incorporation ◽  
Chelating Groups ◽  
Amber Suppression

Bioorthogonal chemistry allows rapid and highly selective reactivity in biological environments. The copper-catalyzed azide–alkyne cycloaddition (CuAAC) is a classic bioorthogonal reaction routinely used to modify azides or alkynes that have been introduced into biomolecules. Amber suppression is an efficient method for incorporating such chemical handles into proteins on the ribosome, in which noncanonical amino acids (ncAAs) are site specifically introduced into the polypeptide in response to an amber (UAG) stop codon. A variety of ncAA structures containing azides or alkynes have been proven useful for performing CuAAC chemistry on proteins. To improve CuAAC efficiency, biologically incorporated alkyne groups can be reacted with azide substrates that contain copper-chelating groups. However, the direct incorporation of copper-chelating azides into proteins has not been explored. To remedy this, we prepared the ncAA paz-lysine (PazK), which contains a picolyl azide motif. We show that PazK is efficiently incorporated into proteins by amber suppression in mammalian cells. Furthermore, PazK-labeled proteins show improved reactivity with alkyne reagents in CuAAC.

scholarly journals Decoupling Protein Production from Cell Growth Enhances the Site-Specific Incorporation of Noncanonical Amino Acids in E. coli

2020 ◽  
Vol 9 (11) ◽  
pp. 3052-3066
Author(s):  
Meritxell Galindo Casas ◽  
Patrick Stargardt ◽  
Juergen Mairhofer ◽  
Birgit Wiltschi
Keyword(s):  
Amino Acids ◽  
Cell Growth ◽  
Protein Production ◽  
Noncanonical Amino Acids ◽  
Site Specific ◽  
E Coli

scholarly journals Genetic Incorporation of Noncanonical Amino Acids Using Two Mutually Orthogonal Quadruplet Codons

2019 ◽  
Vol 8 (5) ◽  
pp. 1168-1174 ◽  
Author(s):  
Erome Daniel Hankore ◽  
Linyi Zhang ◽  
Yan Chen ◽  
Kun Liu ◽  
Wei Niu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Noncanonical Amino Acids ◽  
Genetic Incorporation

The 3′ Codon Context Effect on UAG Suppressor tRNA is Different in Escherichia coli and Human Cells

1993 ◽  
Vol 233 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Mary K. Phillips-Jones ◽  
Fiona J. Watson ◽  
Robin Martin
Keyword(s):  
Escherichia Coli ◽  
Context Effect ◽  
Human Cells ◽  
Suppressor Trna ◽  
Codon Context
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