scholarly journals Single-Plasmid-Based System for Efficient Noncanonical Amino Acid Mutagenesis in Cultured Mammalian Cells

ChemBioChem ◽  
2016 ◽  
Vol 17 (11) ◽  
pp. 1008-1011 ◽  
Author(s):  
Sarit Cohen ◽  
Eyal Arbely
Keyword(s):  
Amino Acid ◽  
Mammalian Cells ◽  
Single Plasmid ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

scholarly journals Defining the current scope and limitations of dual noncanonical amino acid mutagenesis in mammalian cells

2017 ◽  
Vol 8 (10) ◽  
pp. 7211-7217 ◽  
Author(s):  
Yunan Zheng ◽  
Partha Sarathi Addy ◽  
Raja Mukherjee ◽  
Abhishek Chatterjee
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Mammalian Cells ◽  
Noncanonical Amino Acids ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

We systematically evaluate potential platforms for site-specifically incorporating two distinct noncanonical amino acids into proteins expressed in mammalian cells with optimal fidelity and efficiency – a technology that will have many enabling applications.

scholarly journals Expanding the Scope of Single- and Double-Noncanonical Amino Acid Mutagenesis in Mammalian Cells Using Orthogonal Polyspecific Leucyl-tRNA Synthetases

Biochemistry ◽  
2017 ◽  
Vol 57 (4) ◽  
pp. 441-445 ◽  
Author(s):  
Yunan Zheng ◽  
Raja Mukherjee ◽  
Melissa A. Chin ◽  
Peter Igo ◽  
Martin J. Gilgenast ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mammalian Cells ◽  
Trna Synthetases ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

Manipulating Unconventional CH-Based Hydrogen Bonding in a Methyltransferase via Noncanonical Amino Acid Mutagenesis

2014 ◽  
Vol 9 (8) ◽  
pp. 1692-1697 ◽  
Author(s):  
Scott Horowitz ◽  
Upendra Adhikari ◽  
Lynnette M. A. Dirk ◽  
Paul A. Del Rizzo ◽  
Ryan A. Mehl ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Amino Acid ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

Increased gene translation stringency in mammalian cells by nonsense suppression at multiple permissive sites with a single noncanonical amino acid

FEBS Letters ◽  
2020 ◽  
Vol 594 (15) ◽  
pp. 2452-2461
Author(s):  
Lucija Kadunc ◽  
Maja Svetličič ◽  
Vida Forstnerič ◽  
Iva Hafner Bratkovič ◽  
Roman Jerala
Keyword(s):  
Amino Acid ◽  
Mammalian Cells ◽  
Nonsense Suppression ◽  
Noncanonical Amino Acid
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