Potential of Proapoptotic Peptides to Induce the Formation of Giant Plasma Membrane Vesicles with Lipid Domains

ChemBioChem ◽  
2015 ◽  
Vol 16 (9) ◽  
pp. 1288-1292 ◽  
Author(s):  
Daniel Lauster ◽  
Olalla Vazquez ◽  
Roland Schwarzer ◽  
Oliver Seitz ◽  
Andreas Herrmann
Keyword(s):  
Plasma Membrane ◽  
Membrane Vesicles ◽  
Lipid Domains ◽  
Plasma Membrane Vesicles ◽  
Proapoptotic Peptides

scholarly journals Nanodomains can persist at physiologic temperature in plasma membrane vesicles and be modulated by altering cell lipids

2020 ◽  
Vol 61 (5) ◽  
pp. 758-766 ◽  
Author(s):  
Guangtao Li ◽  
Qing Wang ◽  
Shinako Kakuda ◽  
Erwin London
Keyword(s):  
Plasma Membrane ◽  
Phase Separation ◽  
Light Microscopy ◽  
Large Scale ◽  
Membrane Vesicles ◽  
Lipid Domains ◽  
Domain Formation ◽  
Plasma Membrane Vesicles ◽  
Intact Cells ◽  
Wide Range

The formation and properties of liquid-ordered (Lo) lipid domains (rafts) in the plasma membrane are still poorly understood. This limits our ability to manipulate ordered lipid domain-dependent biological functions. Giant plasma membrane vesicles (GPMVs) undergo large-scale phase separations into coexisting Lo and liquid-disordered lipid domains. However, large-scale phase separation in GPMVs detected by light microscopy is observed only at low temperatures. Comparing Förster resonance energy transfer-detected versus light microscopy-detected domain formation, we found that nanodomains, domains of nanometer size, persist at temperatures up to 20°C higher than large-scale phases, up to physiologic temperature. The persistence of nanodomains at higher temperatures is consistent with previously reported theoretical calculations. To investigate the sensitivity of nanodomains to lipid composition, GPMVs were prepared from mammalian cells in which sterol, phospholipid, or sphingolipid composition in the plasma membrane outer leaflet had been altered by cyclodextrin-catalyzed lipid exchange. Lipid substitutions that stabilize or destabilize ordered domain formation in artificial lipid vesicles had a similar effect on the thermal stability of nanodomains and large-scale phase separation in GPMVs, with nanodomains persisting at higher temperatures than large-scale phases for a wide range of lipid compositions. This indicates that it is likely that plasma membrane nanodomains can form under physiologic conditions more readily than large-scale phase separation. We also conclude that membrane lipid substitutions carried out in intact cells are able to modulate the propensity of plasma membranes to form ordered domains. This implies lipid substitutions can be used to alter biological processes dependent upon ordered domains.

Calcium efflux of plasma membrane vesicles exposed to ELF magnetic fields-test of a nuclear magnetic resonance interaction model

2012 ◽  
Vol 33 (7) ◽  
pp. 535-542 ◽  
Author(s):  
Wenjun J. Sun ◽  
Mehri Kaviani Mogadam ◽  
Marianne Sommarin ◽  
Henrietta Nittby ◽  
Leif G. Salford ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Plasma Membrane ◽  
Magnetic Resonance ◽  
Magnetic Fields ◽  
Interaction Model ◽  
Membrane Vesicles ◽  
Resonance Interaction ◽  
Calcium Efflux ◽  
Plasma Membrane Vesicles ◽  
Nuclear Magnetic

Pore-Spanning Plasma Membranes Derived from Giant Plasma Membrane Vesicles

2021 ◽  
Author(s):  
Nikolas K. Teiwes ◽  
Ingo Mey ◽  
Phila C. Baumann ◽  
Lena Strieker ◽  
Ulla Unkelbach ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Plasma Membranes ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles
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