Development of Colorimetric HTS Assay of Cytochrome P450 forortho-Specific Hydroxylation, and Engineering of CYP102D1 with Enhanced Catalytic Activity and Regioselectivity

ChemBioChem ◽  
2013 ◽  
Vol 14 (10) ◽  
pp. 1231-1238 ◽  
Author(s):  
Kwon-Young Choi ◽  
Eun-Ok Jung ◽  
Hyungdon Yun ◽  
Yung-Hun Yang ◽  
Romas J. Kazlauskas ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Catalytic Activity

Opiate-specific effects on mRNA and catalytic activity of a hepatic cytochrome P450 isozyme

1994 ◽  
Vol 53 ◽  
pp. S273-S278 ◽  
Author(s):  
A Rane ◽  
C Bjelfman ◽  
C Thyr ◽  
I Porsch-Hällström
Keyword(s):  
Cytochrome P450 ◽  
Catalytic Activity ◽  
Specific Effects ◽  
Hepatic Cytochrome

scholarly journals An NH2-terminal truncated cytochrome P450 CYP3A4 showing catalytic activity is present in the cytoplasm of human liver cells

2008 ◽  
Vol 40 (2) ◽  
pp. 254 ◽  
Author(s):  
Songhee Jeon ◽  
Keon-Hee Kim ◽  
Chul-Ho Yun ◽  
Boo-Whan Hong ◽  
Yoon-Seok Chang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Catalytic Activity ◽  
Human Liver ◽  
Liver Cells ◽  
Human Liver Cells

scholarly journals Reduced Duodenal Cytochrome P450 3A Protein Expression and Catalytic Activity in Patients With Cirrhosis

2009 ◽  
Vol 85 (4) ◽  
pp. 387-393 ◽  
Author(s):  
DJ McConn II ◽  
YS Lin ◽  
TL Mathisen ◽  
DK Blough ◽  
Y Xu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Catalytic Activity ◽  
Protein Expression ◽  
Cytochrome P450 3A

Trans-resveratrol modulates the catalytic activity and mRNA expression of the procarcinogen-activating human cytochrome P450 1B1

2000 ◽  
Vol 78 (11) ◽  
pp. 874-881 ◽  
Author(s):  
Thomas KH Chang ◽  
Wendy BK Lee ◽  
Hin Hin Ko
Keyword(s):  
Gene Expression ◽  
Cytochrome P450 ◽  
Catalytic Activity ◽  
Pcr Analysis ◽  
Mrna Levels ◽  
Toxic Response ◽  
Cytochrome P450 1B1 ◽  
Cyp1b1 Mrna ◽  
Mcf 7

The present study was performed to determine if trans-resveratrol (3,5,4'-trihydroxy-trans-stilbene) modulates the catalytic activity and gene expression of cytochrome P450 1B1 (CYP1B1). In vitro, trans-resveratrol decreased human recombinant CYP1B1-catalyzed 7-ethoxyresorufin O-dealkylation activity, with an IC50 value of 1.4 ± 0.2 µM (mean ± SEM). Enzyme kinetic analysis indicated that trans-resveratrol inhibited CYP1B1 enzyme activity by a mixed-type inhibition and the apparent Ki was 0.75 ± 0.06 µM. To determine if trans-resveratrol modulates constitutive CYP1B1 gene expression, cultured MCF-7 human breast carcinoma cells were treated with trans-resveratrol. As indicated by RT-PCR analysis, treatment of MCF-7 cells with 10 µM trans-resveratrol decreased relative CYP1B1 mRNA levels after 5 h, but not after 1.5 or 3 h, of exposure. trans-Resveratrol treatment at 5, 7.5, 10, or 20 µM for 5 h produced a concentration-dependent decrease in CYP1B1 mRNA levels. The extent of suppression was ~50% at 20 µM concentration. The suppressive effect was not a consequence of a toxic response to the compound as assessed by a cell proliferation assay. Overall, our novel finding that trans-resveratrol inhibits the catalytic activity and suppresses the constitutive gene expression of CYP1B1 leads to the possibility that this nutraceutical confers protection against toxicity and carcinogenicity induced by compounds that undergo CYP1B1-catalyzed bioactivation.Key words: cytochrome P450, CYP1B1, 7-ethoxyresorufin, nutraceutical, trans-resveratrol.

scholarly journals SHORT COMMUNICATION: Influence of a mutation reducing the catalytic activity of the cytochrome P450 CYP2D6 on lung cancer susceptibility

1996 ◽  
Vol 17 (10) ◽  
pp. 2267-2269 ◽  
Author(s):  
M. Legrand ◽  
L. Stücker ◽  
D. Marez ◽  
N. Sabbagh ◽  
JM. Lo-Guidice ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cytochrome P450 ◽  
Catalytic Activity ◽  
Short Communication ◽  
Cancer Susceptibility ◽  
Lung Cancer Susceptibility

Cytochrome P450 in the house fly: Structure, catalytic activity and regulation of expression ofCYP6 A1in an insecticide-resistant strain

1995 ◽  
Vol 43 (3) ◽  
pp. 233-239 ◽  
Author(s):  
René Feyereisen ◽  
John F. Andersen ◽  
Flerida A. Cariño ◽  
Michael B. Cohen ◽  
Josette F. Koener
Keyword(s):  
Cytochrome P450 ◽  
Catalytic Activity ◽  
Resistant Strain ◽  
House Fly ◽  
Regulation Of Expression
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