scholarly journals The Structure of Glycerol Trinitrate Reductase NerA from Agrobacterium radiobacter Reveals the Molecular Reason for Nitro‐ and Ene‐Reductase Activity in OYE Homologues

ChemBioChem ◽  
2013 ◽  
Vol 14 (7) ◽  
pp. 836-845 ◽  
Author(s):  
Gustav Oberdorfer ◽  
Alexandra Binter ◽  
Silvia Wallner ◽  
Katharina Durchschein ◽  
Mélanie Hall ◽  
...  
Keyword(s):  
Reductase Activity ◽  
Agrobacterium Radiobacter ◽  
Glycerol Trinitrate

scholarly journals Purification, properties, and sequence of glycerol trinitrate reductase from Agrobacterium radiobacter.

1997 ◽  
Vol 179 (24) ◽  
pp. 7796-7802 ◽  
Author(s):  
J R Snape ◽  
N A Walkley ◽  
A P Morby ◽  
S Nicklin ◽  
G F White
Keyword(s):  
Agrobacterium Radiobacter ◽  
Glycerol Trinitrate

scholarly journals Purification and Characterization of Nitrate Reductase (NAR) from Pseudomonas sp. SH7 Isolate

2010 ◽  
Vol 4 (2) ◽  
pp. 23-36
Author(s):  
Shaymaa H. Al-Rajhi ◽  
Majed H. Al-Gelawi ◽  
Ghazi M. Aziz
Keyword(s):  
Nitrate Reductase ◽  
Reductase Activity ◽  
Gel Filtration ◽  
Aerobic Condition ◽  
Maximum Activity ◽  
Heat Sensitivity ◽  
Pseudomonas Sp ◽  
Carbon And Nitrogen ◽  
Glycerol Trinitrate

Sixty five soil samples and fifteen water samples were collected from different places in which previously explosions were occurred in Iraq. Seven isolates showed ability to utilize 0.1mM trinitrotoluene (TNT) and/or 0.2mM glycerol trinitrate (GTN) as a sole carbon and nitrogen source and one of these isolates showed the highest nitrate reduction which was classified and coded as Pseudomonas sp. SH7. The highest nitrate reductase activity extracted by sonication while optimum conditions for enzyme production in minimal media pH 7 containing 0.25mM GTN at 35oC for 3 days under aerobic condition. Nitrate reductase was purified by 40-60% ammonium sulphate, ion exchange and gel filtration. Nitrate reductase molecular weight determined by SDS-PAGE was 115 kD. The characterization of purified enzyme activity and stability was higher at a pH between 6.5-7.5 and. Maximum activity was at 35oC and stable at 30-40oC for 15 min., while for heat sensitivity 100% activity observed at 45oC for 20 min. Treatment with 200 µM azide and 500 µM cyanide inhibited the activity by 76 and 91% respectively.

Lamin B receptor: role on chromatin structure, cellular senescence and possibly aging

2020 ◽  
Vol 477 (14) ◽  
pp. 2715-2720
Author(s):  
Susana Castro-Obregón
Keyword(s):  
Cellular Senescence ◽  
Nuclear Membrane ◽  
Chromatin Structure ◽  
Cholesterol Synthesis ◽  
Reductase Activity ◽  
Chimeric Protein ◽  
Nuclear Lamina ◽  
Lamin B ◽  
Inner Nuclear Membrane ◽  
Lamin B Receptor

The nuclear envelope is composed by an outer nuclear membrane and an inner nuclear membrane, which is underlain by the nuclear lamina that provides the nucleus with mechanical strength for maintaining structure and regulates chromatin organization for modulating gene expression and silencing. A layer of heterochromatin is beneath the nuclear lamina, attached by inner nuclear membrane integral proteins such as Lamin B receptor (LBR). LBR is a chimeric protein, having also a sterol reductase activity with which it contributes to cholesterol synthesis. Lukasova et al. showed that when DNA is damaged by ɣ-radiation in cancer cells, LBR is lost causing chromatin structure changes and promoting cellular senescence. Cellular senescence is characterized by terminal cell cycle arrest and the expression and secretion of various growth factors, cytokines, metalloproteinases, etc., collectively known as senescence-associated secretory phenotype (SASP) that cause chronic inflammation and tumor progression when they persist in the tissue. Therefore, it is fundamental to understand the molecular basis for senescence establishment, maintenance and the regulation of SASP. The work of Lukasova et al. contributed to our understanding of cellular senescence establishment and provided the basis that lead to the further discovery that chromatin changes caused by LBR reduction induce an up-regulated expression of SASP factors. LBR dysfunction has relevance in several diseases and possibly in physiological aging. The potential bifunctional role of LBR on cellular senescence establishment, namely its role in chromatin structure together with its enzymatic activity contributing to cholesterol synthesis, provide a new target to develop potential anti-aging therapies.

Visceral fat drives 5[alpha]-reductase activity independent of BMI in women with polycystic ovarian syndrome

2015 ◽  
Author(s):  
Punith Kempegowda ◽  
Michael W O'Reilly ◽  
Nicola J Crabtree ◽  
Angela E Taylor ◽  
Beverly A Hughes ◽  
...  
Keyword(s):  
Visceral Fat ◽  
Polycystic Ovarian Syndrome ◽  
Reductase Activity ◽  
Ovarian Syndrome
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