In Vitro Selection of Proteins that Undergo Covalent Labeling with Small Molecules by Thiol-Disulfide Exchange by Using Ribosome Display

ChemBioChem ◽  
2011 ◽  
Vol 12 (6) ◽  
pp. 962-969 ◽  
Author(s):  
Hayato Yanagida ◽  
Tomoaki Matsuura ◽  
Yasuaki Kazuta ◽  
Tetsuya Yomo
Keyword(s):  
Small Molecules ◽  
In Vitro Selection ◽  
Covalent Labeling ◽  
Ribosome Display ◽  
Disulfide Exchange ◽  
Selection Of

In vitro selection of other proteins than antibodies by means of ribosome display

FEBS Letters ◽  
2001 ◽  
Vol 502 (1-2) ◽  
pp. 35-40 ◽  
Author(s):  
Thorsten Lamla ◽  
Volker A Erdmann
Keyword(s):  
In Vitro Selection ◽  
Ribosome Display ◽  
Selection Of

In vitro selection of peptide aptamers using a ribosome display for a conducting polymer

2014 ◽  
Vol 117 (4) ◽  
pp. 501-503 ◽  
Author(s):  
Zha Li ◽  
Takanori Uzawa ◽  
Haichao Zhao ◽  
Shyh-Chyang Luo ◽  
Hsiao-hua Yu ◽  
...  
Keyword(s):  
Conducting Polymer ◽  
In Vitro Selection ◽  
Ribosome Display ◽  
Peptide Aptamers ◽  
Selection Of

In vitro selection of a photo-responsive peptide aptamer using ribosome display

2012 ◽  
Vol 48 (97) ◽  
pp. 11871 ◽  
Author(s):  
Mingzhe Liu ◽  
Seiichi Tada ◽  
Mika Ito ◽  
Hiroshi Abe ◽  
Yoshihiro Ito
Keyword(s):  
In Vitro Selection ◽  
Ribosome Display ◽  
Peptide Aptamer ◽  
Selection Of

In vitro selection of peptide aptamers with affinity to single-wall carbon nanotubes using a ribosome display

2012 ◽  
Vol 35 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Zha Li ◽  
Takanori Uzawa ◽  
Takashi Tanaka ◽  
Akira Hida ◽  
Koji Ishibashi ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
In Vitro Selection ◽  
Single Wall Carbon Nanotubes ◽  
Ribosome Display ◽  
Peptide Aptamers ◽  
Single Wall Carbon ◽  
Selection Of

In vitro selection of zinc finger DNA-binding proteins through ribosome display

2006 ◽  
Vol 345 (3) ◽  
pp. 1149-1154 ◽  
Author(s):  
Hiroshi Ihara ◽  
Masayasu Mie ◽  
Hisakage Funabashi ◽  
Fumio Takahashi ◽  
Tatsuya Sawasaki ◽  
...  
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Binding Proteins ◽  
In Vitro Selection ◽  
Dna Binding Proteins ◽  
Ribosome Display ◽  
Selection Of

The Effects of Shock Vancomycin Concentrations on the Formation of Heteroresistance in Staphylococcus aureus

2020 ◽  
Vol 65 (9-10) ◽  
pp. 3-7
Author(s):  
V. V. Gostev ◽  
Yu. V. Sopova ◽  
O. S. Kalinogorskaya ◽  
M. E. Velizhanina ◽  
I. V. Lazareva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Selection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
High Concentration ◽  
Short Term ◽  
High Concentrations ◽  
Selection Of ◽  
Selection Of Resistance ◽  
Test Cultures

Glycopeptides are the basis of the treatment of infections caused by MRSA (Methicillin-Resistant Staphylococcus aureus). Previously, it was demonstrated that antibiotic tolerant phenotypes are formed during selection of resistance under the influence of high concentrations of antibiotics. The present study uses a similar in vitro selection model with vancomycin. Clinical isolates of MRSA belonging to genetic lines ST8 and ST239, as well as the MSSA (ATCC29213) strain, were included in the experiment. Test isolates were incubated for five hours in a medium with a high concentration of vancomycin (50 μg/ml). Test cultures were grown on the medium without antibiotic for 18 hours after each exposure. A total of ten exposure cycles were performed. Vancomycin was characterized by bacteriostatic action; the proportion of surviving cells after exposure was 70–100%. After selection, there was a slight increase in the MIC to vancomycin (MIC 2 μg/ml), teicoplanin (MIC 1.5–3 μg/ml) and daptomycin (MIC 0.25–2 μg/ml). According to the results of PAP analysis, all strains showed an increase in the area under curve depending on the concentration of vancomycin after selection, while a heteroresistant phenotype (with PAP/AUC 0.9) was detected in three isolates. All isolates showed walK mutations (T188S, D235N, E261V, V380I, and G223D). Exposure to short-term shock concentrations of vancomycin promotes the formation of heteroresistance in both MRSA and MSSA. Formation of VISA phenotypes is possible during therapy with vancomycin.

Wheat Germ Cell-Free Translation System as a Tool for In vitro Selection of Functional Proteins

2002 ◽  
Vol 5 (6) ◽  
pp. 473-480
Author(s):  
Bentham Science Publisher A.N. Alexandrov ◽  
Bentham Science Publisher V.Yu. Alakhov ◽  
Bentham Science Publisher A.I. Miroshnikov
Keyword(s):  
Germ Cell ◽  
Wheat Germ ◽  
In Vitro Selection ◽  
Translation System ◽  
Free Translation ◽  
Selection Of
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