Gene Disruption and Biochemical Characterization of Verruculogen Synthase of Aspergillus fumigatus

ChemBioChem ◽  
2011 ◽  
Vol 12 (5) ◽  
pp. 711-714 ◽  
Author(s):  
Naoki Kato ◽  
Hirokazu Suzuki ◽  
Hiroshi Takagi ◽  
Masakazu Uramoto ◽  
Shunji Takahashi ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Gene Disruption ◽  
Biochemical Characterization

scholarly journals Biochemical Characterization of Aspergillus fumigatus AroH, a Putative Aromatic Amino Acid Aminotransferase

2018 ◽  
Vol 5 ◽  
Author(s):  
Mirco Dindo ◽  
Egidia Costanzi ◽  
Marco Pieroni ◽  
Claudio Costantini ◽  
Giannamaria Annunziato ◽  
...  
Keyword(s):  
Amino Acid ◽  
Aspergillus Fumigatus ◽  
Aromatic Amino Acid ◽  
Biochemical Characterization ◽  
Aromatic Amino Acid Aminotransferase

Biochemical Characterization of Cloned Aspergillus fumigatus Phytase (phyA)

2000 ◽  
Vol 275 (2) ◽  
pp. 279-285 ◽  
Author(s):  
Abul H.J. Ullah ◽  
Kandan Sethumadhavan ◽  
X.G. Lei ◽  
Edward J. Mullaney
Keyword(s):  
Aspergillus Fumigatus ◽  
Biochemical Characterization

Production of amylase by soil fungi and partial biochemical characterization of amylase of a selected strain (Aspergillus fumigatus Fresenius)

1993 ◽  
Vol 39 (7) ◽  
pp. 681-685 ◽  
Author(s):  
Claudia M. Domingues ◽  
Rosane M. Peralta
Keyword(s):  
Aspergillus Fumigatus ◽  
Soil Fungi ◽  
Biochemical Characterization ◽  
Amylase Activity ◽  
Carbon Sources ◽  
Lignocellulosic Materials ◽  
Liquid Media ◽  
Maximal Yield ◽  
High Enzymatic Activity

Filamentous fungi from soil were screened for their ability to produce amylases in semisolid and liquid media with wheat bran. A selected strain identified as Aspergillus fumigatus Fresenius showed high enzymatic activity for α-amylase and glucoamylase. The maximal yield of these amylases was obtained when lignocellulosic materials were the carbon sources. The optimal pH and temperature were 6.0 and 50 °C, respectively, for both enzymes. α-Amylase activity was more thermostable than glucoamylase activity.Key words: amylolitic fungi, α-amylase, glucoamylase, Aspergillus fumigatus.

scholarly journals Pathway of Glycine Betaine Biosynthesis in Aspergillus fumigatus

2013 ◽  
Vol 12 (6) ◽  
pp. 853-863 ◽  
Author(s):  
Karine Lambou ◽  
Andrea Pennati ◽  
Isabel Valsecchi ◽  
Rui Tada ◽  
Stephen Sherman ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Glycine Betaine ◽  
Biochemical Characterization ◽  
Choline Oxidase ◽  
Betaine Aldehyde Dehydrogenase ◽  
Metabolic Intermediate ◽  
Content Type ◽  
Betaine Aldehyde ◽  
Functional Features

ABSTRACTThe choline oxidase (CHOA) and betaine aldehyde dehydrogenase (BADH) genes identified inAspergillus fumigatusare present as a cluster specific for fungal genomes. Biochemical and molecular analyses of this cluster showed that it has very specific biochemical and functional features that make it unique and different from its plant and bacterial homologs.A. fumigatusChoAp catalyzed the oxidation of choline to glycine betaine with betaine aldehyde as an intermediate and reduced molecular oxygen to hydrogen peroxide using FAD as a cofactor.A. fumigatusBadhp oxidized betaine aldehyde to glycine betaine with reduction of NAD+to NADH. Analysis of theAfchoAΔ::HPHandAfbadAΔ::HPHsingle mutants and theAfchoAΔAfbadAΔ::HPHdouble mutant showed thatAfChoAp is essential for the use of choline as the sole nitrogen, carbon, or carbon and nitrogen source during the germination process.AfChoAp andAfBadAp were localized in the cytosol of germinating conidia and mycelia but were absent from resting conidia. Characterization of the mutant phenotypes showed that glycine betaine inA. fumigatusfunctions exclusively as a metabolic intermediate in the catabolism of choline and not as a stress protectant. This study inA. fumigatusis the first molecular, cellular, and biochemical characterization of the glycine betaine biosynthetic pathway in the fungal kingdom.

scholarly journals Structural and biochemical characterization of the exopolysaccharide deacetylase Agd3 required for Aspergillus fumigatus biofilm formation

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Natalie C. Bamford ◽  
François Le Mauff ◽  
Jaime C. Van Loon ◽  
Hanna Ostapska ◽  
Brendan D. Snarr ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Biofilm Formation ◽  
Biochemical Characterization

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

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