scholarly journals Effects of Hapten Density on the Induced Antibody Repertoire

ChemBioChem ◽  
2010 ◽  
Vol 11 (12) ◽  
pp. 1686-1691 ◽  
Author(s):  
Qian Li ◽  
Luis G. Rodriguez ◽  
David F. Farnsworth ◽  
Jeffrey C. Gildersleeve
Keyword(s):  
Antibody Repertoire ◽  
Hapten Density

scholarly journals Human sera possess a limited antibody repertoire to influenza neuraminidase antigenic variants selectedin vitro

1984 ◽  
Vol 92 (2) ◽  
pp. 243-250 ◽  
Author(s):  
Anita Natali ◽  
P. F. Panizzi ◽  
C. Chezzi ◽  
J. S. Oxford
Keyword(s):  
Monoclonal Antibody ◽  
H3n2 Virus ◽  
Antibody Repertoire ◽  
Human Sera

SUMMARYFour antigenic variants of the neuraminidase (NA) of A/Texas/77 (H3N2) virus were selected using monoclonal antibody at a frequency of one variant in 105parental virions. The antigenic variants failed to react serologically with the monoclonal antibody used for their selectionin vitro. The antigenic variants failed also to react serologically with a proportion of sera from children and adults although all of the sera reacted with the parental A/Texas/77 virus. Thus, certain human sera have a restricted antibody repertoire to influenza NA antigen which might enable virus antigenic variants to avoid anti-NA antibody-mediated neutralization in nature.

scholarly journals Genetic analysis of self-associating immunoglobulin G rheumatoid factors from two rheumatoid synovia implicates an antigen-driven response.

1992 ◽  
Vol 175 (3) ◽  
pp. 831-842 ◽  
Author(s):  
T Olee ◽  
E W Lu ◽  
D F Huang ◽  
R W Soto-Gil ◽  
M Deftos ◽  
...  
Keyword(s):  
Amino Acid ◽  
Heavy Chain ◽  
Immunoglobulin M ◽  
Natural Antibody ◽  
Antibody Repertoire ◽  
Rheumatoid Factors ◽  
Variable Regions ◽  
Genetic Origins ◽  
V Genes ◽  
Vh Gene

Although much has been learned about the molecular basis of immunoglobulin M (IgM) rheumatoid factors (RFs) in healthy individuals and in patients with mixed cryoglobulinemia and rheumatoid arthritis, little is known about the genetic origins of the potentially pathogenic IgG RFs in the inflamed rheumatoid synovia of patients. Recently, we generated from unmanipulated synovium B cells several hybridomas that secreted self-associating IgG RFs. To delineate the genetic origins of such potentially pathogenic RFs, we adapted the anchored polymerase chain reaction to rapidly clone and characterize the expressed Ig V genes for the L1 and the D1 IgG RFs. Then, we identified the germline counterparts of the expressed L1 IgG RF V genes. The results showed that the L1 heavy chain was encoded by a Vh gene that is expressed preferentially during early ontogenic development, and that is probably located within 240 kb upstream of the Jh locus. The overlap between this RF Vh gene and the restricted fetal antibody repertoire is reminiscent of the natural antibody-associated Vh genes, and suggests that at least part of the "potential pathogenic" IgG RFs in rheumatoid synovium may derive from the "physiological" natural antibody repertoire in a normal immune system. Indeed, the corresponding germline Vh gene for L1 encodes the heavy chain of an IgM RF found in a 19-wk-old fetal spleen. Furthermore, the comparisons of the expressed RF V genes and their germline counterparts reveal that the L1 heavy and light chain variable regions had, respectively, 16 and 7 somatic mutations, which resulted in eight and four amino acid changes. Strikingly, all eight mutations in the complementarity determining regions of the V gene-encoded regions were replacement changes, while only 6 of 11 mutations in the framework regions caused amino acid changes. Combined with L1's high binding affinity toward the Fc fragment, these results suggest strongly that the L1 IgG RF must have been driven by the Fc antigen.

scholarly journals Method development of immunoglobulin G purification from micro-volumes of human serum for untargeted and targeted proteomics-based antibody repertoire studies

2019 ◽  
Vol 27 (2) ◽  
pp. 475-482
Author(s):  
Yu-Ting Chang ◽  
Ming-Chu Chang ◽  
Yun-Jung Tsai ◽  
Christine Ferng ◽  
Hsi-Chang Shih ◽  
...  
Keyword(s):  
Human Serum ◽  
Immunoglobulin G ◽  
Method Development ◽  
Antibody Repertoire ◽  
Targeted Proteomics
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