Isolation of Phospholipase D Mutants Having Phosphatidylinositol-Synthesizing Activity with Positional Specificity onmyo-Inositol

ChemBioChem ◽  
2009 ◽  
Vol 10 (3) ◽  
pp. 559-564 ◽  
Author(s):  
Atsushi Masayama ◽  
Kaori Tsukada ◽  
Chika Ikeda ◽  
Hideo Nakano ◽  
Yugo Iwasaki
Keyword(s):  
Phospholipase D ◽  
Positional Specificity

Directing positional specificity in enzymatic synthesis of bioactive 1-phosphatidylinositol by protein engineering of a phospholipase D

2015 ◽  
Vol 113 (1) ◽  
pp. 62-71 ◽  
Author(s):  
Jasmina Damnjanović ◽  
Chisato Kuroiwa ◽  
Hidetoshi Tanaka ◽  
Ken Ishida ◽  
Hideo Nakano ◽  
...  
Keyword(s):  
Protein Engineering ◽  
Phospholipase D ◽  
Enzymatic Synthesis ◽  
Positional Specificity

Using of Phospholipase D of Corynebacterium Pseudotuberculosis Ovis as Immunostimulant with Some Poultry Vaccines

2015 ◽  
Vol 43 (1) ◽  
pp. 174-181
Author(s):  
Marwah M ◽  
Hanan Mahmoud ◽  
Mona El-Shehedi ◽  
Namaa Mohamed
Keyword(s):  
Phospholipase D ◽  
Corynebacterium Pseudotuberculosis

Role of Phospholipase D Inhibitor in Regulating Expression of Senescencerelated Phospholipase D gene in Postharvest Longan Fruit

2019 ◽  
Vol 14 (7) ◽  
pp. 649-657 ◽  
Author(s):  
Li Li ◽  
Jiemin Li ◽  
Jian Sun ◽  
Ping Yi ◽  
Changbao Li ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Ascorbic Acid Content ◽  
Titratable Acidity ◽  
Significant Inhibition ◽  
Solid Content ◽  
Total Soluble Solid ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Soluble Solid ◽  
Pericarp Browning

Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit. Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature. Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit. Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage. Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.

scholarly journals Release of dialkylglycerol from purple membrane phospholipids by phospholipase D.

1990 ◽  
Vol 265 (11) ◽  
pp. 5956-5959
Author(s):  
A Muga ◽  
J L Arrondo ◽  
J I Gurtubay ◽  
F M Goñi
Keyword(s):  
Phospholipase D ◽  
Purple Membrane ◽  
Membrane Phospholipids
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