The Role of the Conserved Threonine in P450BM3 Oxygen Activation: Substrate-Determined Hydroxylation Activity of the Thr268Ala Mutant

ChemBioChem ◽  
2008 ◽  
Vol 9 (2) ◽  
pp. 261-266 ◽  
Author(s):  
Max J. Cryle ◽  
James J. De Voss
Keyword(s):  
Oxygen Activation ◽  
Hydroxylation Activity

Cryptochrome 2 is involved in betacyanin decomposition induced by blue light in Suaeda salsa

2006 ◽  
Vol 33 (7) ◽  
pp. 697 ◽  
Author(s):  
Wang Chang-Quan ◽  
Liu Tao
Keyword(s):  
Western Blot ◽  
Blue Light ◽  
Hypocotyl Elongation ◽  
Suaeda Salsa ◽  
Biosynthesis Pathway ◽  
Tyrosine Hydroxylation ◽  
Key Enzyme ◽  
Cryptochrome 2 ◽  
Hydroxylation Activity

Seeds of the halophyte Suaeda salsa (L.) Pall. were cultured in 24 h dark and 14 h blue light / 10 h dark to examine the role of blue light and the blue-light-absorbing photoreceptor cryptochrome 2 (CRY2) in betacyanin accumulation, hypocotyl elongation and cotyledon opening in S. salsa seedlings. Darkness significantly promoted betacyanin accumulation and hypocotyl elongation but inhibited cotyledon opening. Blue light suppressed betacyanin accumulation and hypocotyl elongation but stimulated cotyledon opening. Betacyanin in S. salsa seedlings decomposed with time in blue light. Western blot analysis showed that CRY2 protein accumulated both in hypocotyls and cotyledons of S. salsa seedlings grown in dark, but degraded with time in blue light, which was paralleled by a decrease of tyrosine hydroxylation activity of tyrosinase, a key enzyme involved in the betalain biosynthesis pathway. These results suggest that CRY2 protein mediates betacyanin decomposition via inactivation of tyrosinase in S. salsa seedlings, and the blue-light-dependent degradation of CRY2 protein is crucial to its function.

scholarly journals The Key Role of Nanocasting in Gold‐based Fe 2 O 3 Nanocasted Catalysts for Oxygen Activation at the Metal‐support Interface

ChemCatChem ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1915-1927 ◽  
Author(s):  
Tomás García ◽  
José M. López ◽  
Benjamín Solsona ◽  
Rut Sanchis ◽  
David J. Willock ◽  
...  
Keyword(s):  
Oxygen Activation ◽  
Metal Support

Selective hydroxylation of naphthalene using the H2O2-dependent engineered P450BM3 driven by dual-functional small molecules

2018 ◽  
Vol 22 (09n10) ◽  
pp. 831-836 ◽  
Author(s):  
Zhifeng Chen ◽  
Jie Chen ◽  
Nana Ma ◽  
Haifeng Zhou ◽  
Zhiqi Cong
Keyword(s):  
Small Molecules ◽  
Aromatic Hydrocarbons ◽  
Unique Role ◽  
Potential Approach ◽  
Dual Functional ◽  
Selective Hydroxylation ◽  
Hydroxylation Activity

We herein report the H2O2-dependent selective hydroxylation of naphthalene catalyzed by engineered P450BM3 with the assistance of dual-functional small molecules (DFSMs). The mutation at position 268 significantly improved the hydroxylation activity of P450BM3, which is quite different from those engineered P450BM3 peroxygenases and NADPH-dependent P450BM3 mutants previously reported, implicating the unique role of the residue at position 268. This study provides a potential approach to develop the practical hydroxylation biocatalyst of P450s for aromatic hydrocarbons using the DFSM-facilitated P450BM3-H2O2 system.

scholarly journals A quadruple mutant of Arabidopsis reveals a β-carotene hydroxylation activity for LUT1/CYP97C1 and a regulatory role of xanthophylls on determination of the PSI/PSII ratio

2012 ◽  
Vol 12 (1) ◽  
pp. 50 ◽  
Author(s):  
Alessia Fiore ◽  
Luca Dall'Osto ◽  
Stefano Cazzaniga ◽  
Gianfranco Diretto ◽  
Giovanni Giuliano ◽  
...  
Keyword(s):  
Regulatory Role ◽  
Quadruple Mutant ◽  
Β Carotene ◽  
Hydroxylation Activity

ChemInform Abstract: The Role of Individual Bleomycin Thiazoles in Oxygen Activation and DNA Cleavage.

ChemInform ◽  
2010 ◽  
Vol 23 (45) ◽  
pp. no-no
Author(s):  
N. HAMAMICHI ◽  
A. NATRAJAN ◽  
S. M. HECHT
Keyword(s):  
Dna Cleavage ◽  
Oxygen Activation

The role of Thr268 in oxygen activation of cytochrome P450BM-3

Biochemistry ◽  
1995 ◽  
Vol 34 (45) ◽  
pp. 14733-14740 ◽  
Author(s):  
Hyeyeong Yeom ◽  
Stephen G. Sligar ◽  
Huiying Li ◽  
Thomas L. Poulos ◽  
Armand J. Fulco
Keyword(s):  
Oxygen Activation

scholarly journals Role of Hydrogen and Oxygen Activation over Pt and Pd-Doped Composites for Catalytic Hydrogen Combustion

2016 ◽  
Vol 9 (23) ◽  
pp. 19380-19388 ◽  
Author(s):  
Satyapaul A. Singh ◽  
Karan Vishwanath ◽  
Giridhar Madras
Keyword(s):  
Oxygen Activation ◽  
Hydrogen Combustion ◽  
Catalytic Hydrogen
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