Prediction of theCandida antarctica Lipase A Protein Structure by Comparative Modeling and Site-Directed Mutagenesis

ChemBioChem ◽  
2007 ◽  
Vol 8 (12) ◽  
pp. 1409-1415 ◽  
Author(s):  
Alex Kasrayan ◽  
Marco Bocola ◽  
Anders G. Sandström ◽  
Gaston Lavén ◽  
Jan-E. Bäckvall
Keyword(s):  
Protein Structure ◽  
Comparative Modeling ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Lipase A

Prediction of the Candida antarctica Lipase A Protein Structure by Comparative Modeling and Site-Directed Mutagenesis.

ChemInform ◽  
2007 ◽  
Vol 38 (44) ◽  
Author(s):  
Alex Kasrayan ◽  
Marco Bocola ◽  
Anders G. Sandstroem ◽  
Gaston Laven ◽  
Jan-E. Baeckvall
Keyword(s):  
Protein Structure ◽  
Comparative Modeling ◽  
Candida Antarctica ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Candida Antarctica Lipase ◽  
Lipase A

scholarly journals Prediction of theCandida antarcticaLipase A Protein Structure by Comparative Modeling and Site-Directed Mutagenesis

ChemBioChem ◽  
2008 ◽  
Vol 9 (16) ◽  
pp. 2559-2559 ◽  
Author(s):  
Alex Kasrayan ◽  
Marco Bocola ◽  
Anders G. Sandström ◽  
Gaston Lavén ◽  
Jan-E. Bäckvall
Keyword(s):  
Protein Structure ◽  
Comparative Modeling ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

Enhancing activity and thermostability of lipase A from Serratia marcescens by site-directed mutagenesis

2016 ◽  
Vol 93-94 ◽  
pp. 18-28 ◽  
Author(s):  
Mohsen Mohammadi ◽  
Zargham Sepehrizadeh ◽  
Azadeh Ebrahim-Habibi ◽  
Ahmad Reza Shahverdi ◽  
Mohammad Ali Faramarzi ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Lipase A

scholarly journals Dissecting capture and twisting of aureolysin and pseudolysin: functional amino acids of the Dispase autolysis-inducing protein

2020 ◽  
Vol 477 (13) ◽  
pp. 2595-2606
Author(s):  
David Fiebig ◽  
Anita Anderl ◽  
Sahra Al Djaizani ◽  
Harald Kolmar ◽  
Hans-Lothar Fuchsbauer
Keyword(s):  
Amino Acids ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Protein Structure ◽  
Complex Formation ◽  
Disulfide Bonds ◽  
Complete Inhibition ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Streptomyces Mobaraensis

The Dispase autolysis-inducing protein (DAIP) from Streptomyces mobaraensis attracts M4 metalloproteases, which results in inhibition and autolysis of bacillolysin (BL) and thermolysin (TL). The present study shows that aureolysin (AL) from Staphylococcus aureus and pseudolysin (LasB) from Pseudomonas aeruginosa are likewise impaired by DAIP. Complete inhibition occurred when DAIP significantly exceeded the amount of the target protease. At low DAIP concentrations, AL and BL performed autolysis, while LasB and TL degradation required reductants or detergents that break intramolecular disulfide bonds or change the protein structure. Site directed mutagenesis of DAIP and removal of an exposed protein loop either influenced binding or inhibition of AL and TL but had no effect on LasB and BL. The Y170A and Δ239–248 variants had completely lost affinity for TL and AL. The exchange of Asn-275 also impaired the interaction of DAIP with AL. In contrast, DAIP Phe-297 substitution abolished inhibition and autolysis of both target proteases but still allowed complex formation. Our results give rise to the conclusion that other, yet unknown DAIP amino acids inactivate LasB and BL. Obviously, various bacteria in the same habitat caused Streptomyces mobaraensis to continuously optimize DAIP in inactivating the tackling metalloproteases.

9. Enzymes and genes—new horizons

2020 ◽  
pp. 128-148
Author(s):  
Paul Engel
Keyword(s):  
Protein Structure ◽  
Amino Acid ◽  
Protein Engineering ◽  
Molecular Genetics ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
New Horizons ◽  
Pure Research ◽  
Different Parts

‘Enzymes and genes—new horizons’ studies molecular genetics. It details how the method of ‘site-directed mutagenesis’ opened the door to what rapidly became known as protein engineering. Initially, people wondered whether mutated proteins would fold up properly. Occasionally they did not, but, overall, protein engineering has been remarkably successful and has become a powerful tool for pure research, allowing one to test the contribution of different parts of a protein structure with surgical precision. Amino acid dehydrogenases are a family of enzymes. There have been three breakthrough discoveries in modern molecular genetics that rely on remarkable enzymes. These discoveries have transformed biotechnology, biology, and people’s lives.

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