Intracellular Imaging of HCV RNA and Cellular Lipids by Using Simultaneous Two-Photon Fluorescence and Coherent Anti-Stokes Raman Scattering Microscopies

ChemBioChem ◽  
2006 ◽  
Vol 7 (12) ◽  
pp. 1895-1897 ◽  
Author(s):  
Xiaolin Nan ◽  
Angela M. Tonary ◽  
Albert Stolow ◽  
X. Sunney Xie ◽  
John Paul Pezacki
Keyword(s):  
Raman Scattering ◽  
Hcv Rna ◽  
Intracellular Imaging ◽  
Two Photon ◽  
Cellular Lipids ◽  
Two Photon Fluorescence ◽  
Anti Stokes ◽  
Photon Fluorescence

scholarly journals Experimental study on two-photon fluorescence and coherent anti-Stokes Raman scattering microscopy

2017 ◽  
Vol 66 (10) ◽  
pp. 104204
Author(s):  
Hou Guo-Hui ◽  
Luo Teng ◽  
Chen Bing-Ling ◽  
Liu Jie ◽  
Lin Zi-Yang ◽  
...  
Keyword(s):  
Experimental Study ◽  
Raman Scattering ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Anti Stokes ◽  
Photon Fluorescence

scholarly journals Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Takeo Minamikawa ◽  
Yoshinori Murakami ◽  
Naokazu Matsumura ◽  
Hirohiko Niioka ◽  
Shuichiro Fukushima ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Cell Viability ◽  
Near Infrared ◽  
Cell Damage ◽  
Pulsed Laser ◽  
Excitation Scheme ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Anti Stokes ◽  
Photon Fluorescence

In this study, we investigated photo-induced damage to living cells during single- and multifocus excitations for coherent anti-Stokes Raman scattering (CARS) imaging. A near-infrared pulsed laser (709 nm) was used to induce cell damage. We compared the photo-induced cell damage in the single- and the multifocus excitation schemes with the condition to obtain the same CARS signal in the same frame rate. For the evaluation of cell viability, we employed 4′,6-diamidino-2-phenylindole (DAPI) fluorophores that predominantly stained the damaged cells. One- and two-photon fluorescence of DAPI fluorophores were, respectively, excited by an ultraviolet light source and the same near-infrared light source and were monitored to evaluate the cell viability during near-infrared pulsed laser irradiation. We found lower uptake of DAPI fluorophores into HeLa cells during the multifocus excitation compared with the single-focus excitation scheme in both the one- and the two-photon fluorescence examinations. This indicates a reduction of photo-induced cell damage in the multifocus excitation. Our findings suggested that the multifocus excitation scheme is expected to be suitable for CARS microscopy in terms of minimal invasiveness.

scholarly journals NON-LINEAR OPTICAL IMAGING OF OBESITY-RELATED HEALTH RISKS: REVIEW

2009 ◽  
Vol 02 (01) ◽  
pp. 9-25 ◽  
Author(s):  
THUC T. LE ◽  
JI-XIN CHENG
Keyword(s):  
Optical Imaging ◽  
Health Risks ◽  
Sum Frequency ◽  
Two Photon ◽  
Cars Microscopy ◽  
Non Linear ◽  
Linear Optical ◽  
Two Photon Fluorescence ◽  
Anti Stokes ◽  
Photon Fluorescence

This review highlights the recent applications of non-linear optical (NLO) microscopy to study obesity-related health risks. A strong emphasis is given to the applications of coherent anti-Stokes Raman scattering (CARS) microscopy where multiple non-linear optical imaging modalities including CARS, sum-frequency generation (SFG), and two-photon fluorescence are employed simultaneously on a single microscope platform. Specific examples on applications of NLO microscopy to study lipid-droplet biology, obesity-cancer relationship, atherosclerosis, and lipid-rich biological structures are discussed.

Intracellular imaging of host-pathogen interactions using combined CARS and two-photon fluorescence microscopies

2010 ◽  
Vol 3 (3) ◽  
pp. 138-146 ◽  
Author(s):  
Iain Robinson ◽  
Michael Andreas Ochsenkühn ◽  
Colin J. Campbell ◽  
Gerard Giraud ◽  
William J. Hossack ◽  
...  
Keyword(s):  
Host Pathogen Interactions ◽  
Intracellular Imaging ◽  
Two Photon ◽  
Host Pathogen ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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