Tetrabromocinnamic Acid (TBCA) and Related Compounds Represent a New Class of Specific Protein Kinase CK2 Inhibitors

ChemBioChem ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 129-139 ◽  
Author(s):  
Mario A. Pagano ◽  
Giorgia Poletto ◽  
Giovanni Di Maira ◽  
Giorgio Cozza ◽  
Maria Ruzzene ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Specific Protein ◽  
New Class ◽  
Kinase Ck2 ◽  
Related Compounds ◽  
Ck2 Inhibitors ◽  
Specific Protein Kinase

Unprecedented Selectivity and Structural Determinants of a New Class of Protein Kinase CK2 Inhibitors in Clinical Trials for the Treatment of Cancer

Biochemistry ◽  
2011 ◽  
Vol 50 (39) ◽  
pp. 8478-8488 ◽  
Author(s):  
Roberto Battistutta ◽  
Giorgio Cozza ◽  
Fabrice Pierre ◽  
Elena Papinutto ◽  
Graziano Lolli ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Structural Determinants ◽  
New Class ◽  
Kinase Ck2 ◽  
Ck2 Inhibitors

scholarly journals Design, synthesis and evaluation of 2-phenylisothiazolidin-3-one-1,1-dioxides as a new class of human protein kinase CK2 inhibitors

2013 ◽  
Vol 29 (3) ◽  
pp. 338-343 ◽  
Author(s):  
Maksym O. Chekanov ◽  
Olga V. Ostrynska ◽  
Anatoliy R. Synyugin ◽  
Volodymyr G. Bdzhola ◽  
Sergiy M. Yarmoluk
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Human Protein ◽  
Design Synthesis ◽  
New Class ◽  
Human Protein Kinase ◽  
Kinase Ck2 ◽  
Ck2 Inhibitors

scholarly journals Impact of protein kinase CK2 inhibitors on proliferation and differentiation of neural stem cells

Heliyon ◽  
2017 ◽  
Vol 3 (6) ◽  
pp. e00318 ◽  
Author(s):  
Melanie Bender ◽  
Lisa Schwind ◽  
David Grundmann ◽  
Monika Martin ◽  
Markus Klotz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Protein Kinase ◽  
Neural Stem Cells ◽  
Protein Kinase Ck2 ◽  
Proliferation And Differentiation ◽  
Kinase Ck2 ◽  
Ck2 Inhibitors

scholarly journals Structural Determinants of CX-4945 Derivatives as Protein Kinase CK2 Inhibitors: A Computational Study

2011 ◽  
Vol 12 (10) ◽  
pp. 7004-7021 ◽  
Author(s):  
Hongbo Liu ◽  
Xia Wang ◽  
Jian Wang ◽  
Jinghui Wang ◽  
Yan Li ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Computational Study ◽  
Structural Determinants ◽  
Kinase Ck2 ◽  
Ck2 Inhibitors

Indeno[1,2-b]indole derivatives as a novel class of potent human protein kinase CK2 inhibitors

2012 ◽  
Vol 20 (7) ◽  
pp. 2282-2289 ◽  
Author(s):  
Claas Hundsdörfer ◽  
Hans-Jörg Hemmerling ◽  
Claudia Götz ◽  
Frank Totzke ◽  
Patrick Bednarski ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Human Protein ◽  
Indole Derivatives ◽  
Human Protein Kinase ◽  
Kinase Ck2 ◽  
Ck2 Inhibitors

scholarly journals Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) induces apoptosis and caspase-dependent degradation of haematopoietic lineage cell-specific protein 1 (HS1) in Jurkat cells

2002 ◽  
Vol 364 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Maria RUZZENE ◽  
Daniele PENZO ◽  
Lorenzo A. PINNA
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Specific Inhibitor ◽  
Specific Protein ◽  
Jurkat Cells ◽  
Similar Data ◽  
Caspase Cleavage ◽  
Protein Substrates ◽  
Kinase Ck2

Incubation of Jurkat cells with 4,5,6,7-tetrabromobenzotriazole (TBB), a specific inhibitor of protein kinase CK2, induces dose-and time-dependent apoptosis as judged by several criteria. TBB-promoted apoptosis is preceded by inhibition of Ser/Thr phosphorylation of haematopoietic lineage cell-specific protein 1 (HS1) and is accompanied by caspase-dependent fragmentation of the same protein. Both effects are also observable if apoptosis is promoted by anti-Fas antibodies and by etoposide. Moreover, in vitro experiments show that HS1, once phosphorylated by CK2, becomes refractory to cleavage by caspase-3. These findings, in conjunction with similar data in the literature concerning two other CK2 protein substrates, Bid and Max, suggest that CK2 may play a general anti-apoptotic role through the generation of phosphorylated sites conferring resistance to caspase cleavage.

Down-Regulation of NF-κB and STAT3 Signaling Pathways Upon Protein Kinase CK2 Inhibition Empowers Bortezomib-Induced Multiple Myeloma Cell-Death

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 1849-1849
Author(s):  
Sabrina Manni ◽  
Alessandra Brancalion ◽  
Quotti Tubi Laura ◽  
Anna Cabrelle ◽  
Livio Trentin ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Membrane Potential ◽  
Protein Kinase ◽  
Cell Apoptosis ◽  
Mitochondrial Membrane ◽  
Protein Kinase Ck2 ◽  
Plasma Cells ◽  
Signalling Pathways ◽  
Kinase Ck2 ◽  
Ck2 Inhibitors

Abstract Abstract 1849 Multiple Myeloma (MM) malignant plasma cells can be induced to die by blocking the proteasome. Bortezomib (BZ), a first-in class proteasome inhibitor of wide clinical use in MM patients, causes MM cell apoptosis through different mechanisms; however, the means of resistance to its effects are poorly recognized. Our group identified protein kinase CK2 as a critical survival molecule for MM cells (Piazza FA et al., 2006, Blood, 108(5):1698–707). This kinase regulates pivotal apoptosis-related pathways in cancer cells; however, it is currently unknown whether CK2 could be involved downstream proteasome inhibition. Intriguingly, phase I clinical trials are currently ongoing with an oral ATP-competitive CK2 inhibitor in MM and other tumors. We have here sought to investigate whether CK2 takes part in BZ-induced MM cell apoptosis and we studied whether blocking CK2 could influence pro-survival signalling pathways, which could account for MM cell resistance to BZ and chemotherapy. MM cell lines U-266, RPMI-8226 and INA-6, human bone marrow stromal cells and freshly isolated plasma cells from patients were cultured and exposed to BZ and CK2 inhibitors K27 and CX4945 for different time points. Annexin V and propidium iodide staining, evaluation of mitochondrial membrane potential depolarization and western blot (WB) analysis of PARP cleavage and apoptosis-related proteins expression were the assays employed to assess cell growth and viability upon the different treatments. We found that the rate of BZ-induced MM cell apoptosis was significantly increased by the simultaneous inhibition of CK2 and the proteasome in all the MM models tested and mitochondrial membrane potential measurements revealed that CK2 inhibition enhanced BZ-triggered intrinsic apoptotic cascade. Importantly, the combination of CK2 inhibitors and BZ resulted in a synergic growth-suppressive action. WB and RT-PCR analysis revealed that survival-signalling pathways associated with STAT3 and NF-κB were activated by BZ, which also caused a rise in the levels of the unfolded protein response-associated kinase/endoribonuclease IRE1α. These effects could represent unwanted side consequences of BZ treatment and could lend MM cells the ability to escape the cytotoxic effects of this drug. CK2 inhibition produced a strong reduction of phospho Ser 536 and phospho Ser 529 p65 NF-κB subunit, phospho Ser 727 STAT3 and IRE1α levels in MM cells. Remarkably, the simultaneous treatment with BZ and CK2 inhibitors was accompanied by a significant reduction of BZ-triggered p65 NF-κB and STAT3 activation and IRE1α protein levels. These results indicate that protein kinase CK2 protects from BZ-induced apoptosis and modulates pivotal signaling pathways in MM cells, such as the NF-κB and STAT3 cascades, which could otherwise be exploited in the selection of BZ-resistant MM cell clones. Our findings suggest that CK2 inhibition could offer a rational therapeutic option when designing novel BZ-based anti-MM combination therapies. Disclosures: No relevant conflicts of interest to declare.

Structure-Based Design of Novel Potent Protein Kinase CK2 (CK2) Inhibitors with Phenyl-azole Scaffolds

2012 ◽  
Vol 55 (6) ◽  
pp. 2899-2903 ◽  
Author(s):  
Zengye Hou ◽  
Isao Nakanishi ◽  
Takayoshi Kinoshita ◽  
Yoshinori Takei ◽  
Misato Yasue ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Kinase Ck2 ◽  
Ck2 Inhibitors
Sign in / Sign up

Export Citation Format

Share Document