Effects of polyamines on DNA synthesis using various subcellular DNA polymerases extracted from normal rat liver, tumour-bearing rat liver, and tumour cells

2001 ◽  
Vol 19 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Takahiko Taguchi ◽  
Sumiko Kurata ◽  
Mochihiko Ohashi
Keyword(s):  
Dna Synthesis ◽  
Rat Liver ◽  
Dna Polymerases ◽  
Tumour Cells ◽  
Liver Tumour ◽  
Tumour Bearing ◽  
Normal Rat

scholarly journals The role of mitochondria in modifying the cellular ionic environment. Calcium-induced respiratory activities in mitochondria isolated from various tumour cells

1974 ◽  
Vol 144 (3) ◽  
pp. 551-558 ◽  
Author(s):  
R F W Thorne ◽  
F L Bygrave
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Tumour Cells ◽  
Ascites Tumour ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Tumour ◽  
Normal Rat ◽  
Cyclic Stimulation ◽  
Ascites Tumour Cells ◽  
Ehrlich Ascites Tumour Cells

Cyclic stimulation by Ca2+of respiration in mitochondria isolated from Ehrlich ascites-tumour cells occurs only when low phosphate concentrations (approx. 0.5mm) are also included in the incubation system. Under these circumstances the extra oxygen consumed is related stoicheiometrically to the amount of Ca2+taken up by the mitochondria; the values are similar to those obtained with mitochondria from rat liver in the absence of added phosphate. In contrast with liver mitochondria, up to 280nmol of Ca2+/mg of protein can be added to ascites mitochondria without causing any deleterious effect. Respiration in mitochondria isolated from the Yoshida ascites hepatoma (HA 130) and from the Morris hepatomas 5123C and 9618A is also stimulated by Ca2+in a cyclic manner. However, that in mitochondria from regenerating rat liver responds to Ca2+in the same way as those from normal rat liver. ADP-stimulated respiration in mitochondria from Ehrlich ascites tumour cells, but not from rat liver, is inhibited by low amounts of Ca2+.

Methionine synthase activity and sulphur amino acid levels in the rat liver tumour cells HTC and Phi-1

2002 ◽  
Vol 63 (3) ◽  
pp. 381-391 ◽  
Author(s):  
Susan H Kenyon ◽  
Catherine J Waterfield ◽  
John A Timbrell ◽  
Anna Nicolaou
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Tumour Cells ◽  
Liver Tumour ◽  
Methionine Synthase ◽  
Sulphur Amino Acid ◽  
Amino Acid Levels

Effect of orotic acid on in vivo DNA synthesis in hepatocytes of normal rat liver and in hepatic foci/nodules

1993 ◽  
Vol 14 (5) ◽  
pp. 907-912 ◽  
Author(s):  
A. Sheikh ◽  
A. Yusuf ◽  
E. Laconi ◽  
P.M. Rao ◽  
S. Rajalakshmi ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Rat Liver ◽  
Orotic Acid ◽  
Normal Rat

scholarly journals Studies on lipid peroxidation in normal and tumour tissues. The Novikoff rat liver tumour

1986 ◽  
Vol 235 (2) ◽  
pp. 507-514 ◽  
Author(s):  
K H Cheeseman ◽  
M Collins ◽  
K Proudfoot ◽  
T F Slater ◽  
G W Burton ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Rat Liver ◽  
Fatty Acid Content ◽  
Normal Liver ◽  
Rat Hepatocytes ◽  
Tumour Cells ◽  
Alpha Tocopherol ◽  
Polyunsaturated Fatty Acid Content ◽  
Normal Rat

A study has been made of the factors that contribute to the decreased rates of lipid peroxidation under different pro-oxidant conditions in intact Novikoff tumour cells, and in microsomal suspensions prepared from Novikoff tumour cells, compared with isolated normal rat hepatocytes and microsomal suspensions prepared from normal rat liver. The pro-oxidant conditions were the addition of either NADPH, NADPH + ADP + iron, NADPH + CCl4 or ascorbate+iron to the experimental systems used, or exposure to gamma-radiation. Contributory factors to the lower rates of lipid peroxidation observed include: a significant decrease in the polyunsaturated fatty acid content of Novikoff cells or Novikoff microsomes; the decreases are especially marked for the C20:4 and C22:6 fatty acids; a very marked reduction in NADPH-cytochrome c reductase; and no detectable content of cytochrome P-450. Another, and in our opinion critical, contribution to the diminished rate of lipid peroxidation in the tumour material is the substantial increase in alpha-tocopherol relative both to total lipid and to methylene-interrupted double bonds in fatty acids. Moreover, the alpha-tocopherol is the major contributor to lipid-soluble chain-breaking antioxidant in lipid extracts of normal liver and of Novikoff tumour material.

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