Discrepancy between the initial DNA damage and cell survival after camptothecin treatment in two murine lymphoma L5178Y sublines

1996 ◽  
Vol 14 (3) ◽  
pp. 163-171 ◽  
Author(s):  
Iwona Grądzka ◽  
Irena Szumiel
Keyword(s):  
Dna Damage ◽  
Cell Survival ◽  
Murine Lymphoma ◽  
Camptothecin Treatment

scholarly journals Temporal regulation of the Mus81-Mms4 endonuclease ensures cell survival under conditions of DNA damage

2013 ◽  
Vol 41 (19) ◽  
pp. 8943-8958 ◽  
Author(s):  
Irene Saugar ◽  
María Victoria Vázquez ◽  
María Gallo-Fernández ◽  
María Ángeles Ortiz-Bazán ◽  
Mónica Segurado ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Survival ◽  
Temporal Regulation

scholarly journals Gold Nanoparticles Radio-sensitize and Reduce Cell Survival in Lewis Lung Carcinoma

2020 ◽  
Author(s):  
Arvind Pandey ◽  
Veronica Vighetto ◽  
Nicola Di Marzio ◽  
Matteo Hirsch ◽  
Nicola Ferrante ◽  
...  
Keyword(s):  
Dna Damage ◽  
Particle Size ◽  
Gold Nanoparticles ◽  
Cell Survival ◽  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Lewis Lung ◽  
Lung Carcinoma Cells ◽  
Reduce Cell Survival

It has been suggested that particle size plays an important role in determining the genotoxicity of gold nanoparticles (GNPs). The purpose of this study was to compare the potential radio-sensitization effects of two different sized GNPs (3.9 and 37.4 nm) fabricated and examined in vitro in Lewis Lung carcinoma (LLC) as a model of non-small cell lung cancer through use of comet and clonogenic assays. After the treatment of 2Gy X-ray irradiation, both particle sizes demonstrated increased DNA damage when compared to treatment with particles only and radiation alone. This radio-sensitization was further translated into a reduction in cell survival demonstrated by clonogenicity. This work indicates that GNPs of both sizes induce DNA damage in LLC cells at the tested concentrations, whereas the 37.4 nm particle size treatment group demonstrated greater significance in vitro. The presented data aids in the evaluation of the radiobiological response of Lewis Lung carcinoma cells treated with gold nanoparticles.

Cellular damage in vitro

2009 ◽  
Vol 48 (05) ◽  
pp. 208-214 ◽  
Author(s):  
J. Drechsel ◽  
R. Freudenberg ◽  
R. Runge ◽  
G. Wunderlich ◽  
J. Kotzerke ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Survival ◽  
Cell Line ◽  
Cellular Damage ◽  
Thyroid Cell ◽  
Response Curves ◽  
Beta Emitters ◽  
Thyroid Cell Line ◽  
Clonogenic Cell ◽  
Radionuclide Uptake

Summary Aim: The cellular damage of ionising radiation depends on dose, physical radiation quality (e. g. LET) and intracellular radionuclide uptake. The influence of two beta emitters (188Re and 131I) on the thyroid cell line PC Cl3 was studied. Furthermore, we analysed the effect of intracellular accumulation. Methods: The thyroid cell line PC Cl3 was irradiated with 188Re-perrhenate or 131I-sodium iodide in presence or absence of perchlorate. The initial DNA-damage was measured in the comet assay as olive tail moment (OTM). The colony forming assay detects the clonogenic cell survival as surviving fraction. Additional the intracellular radionuclide uptake was quantified. Results: Dose response curves were established for irradiation with 188Re-perrhenate or 131I-iodine under various extra- and intracellular activity distribution conditions. In the presence of perchlorate DNA-damage and clonogenic cell survival for both radionuclides were comparable. In the absence of perchlorat radionuclide uptake of 1.39% (131I) and 4.14% (188Re) were measured causing twofold higher radiotoxicity. Although 131I uptake was lower than 188Re uptake the OTM values were higher und surviving fractions were lower. Conclusions: 131I, compared to 188Re, has lower mean beta energy and a higher LET, and therefore, it induced a higher DNA-damage even at lower intracellular uptake. An additional explanation for the higher radiotoxicity of 131I could be the higher dose exposition caused by crossfire through neighborhood cells.

scholarly journals Loss of PIDD limits NF-κB activation and cytokine production but not cell survival or transformation after DNA damage

2012 ◽  
Vol 20 (4) ◽  
pp. 546-557 ◽  
Author(s):  
F J Bock ◽  
G Krumschnabel ◽  
C Manzl ◽  
L Peintner ◽  
M C Tanzer ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Survival ◽  
Cytokine Production

The interaction between bleomycin and radiation on cell survival and DNA damage in mammalian cell cultures

1985 ◽  
Vol 11 (12) ◽  
pp. 2125-2131 ◽  
Author(s):  
De-Zheng Wu ◽  
Yu-Qin Zhang ◽  
Peter Keng ◽  
Robert M. Sutherland ◽  
Louis Lasagna
Keyword(s):  
Dna Damage ◽  
Cell Survival ◽  
Mammalian Cell ◽  
Cell Cultures ◽  
Mammalian Cell Cultures

scholarly journals A theoretical cell-killing model to evaluate oxygen enhancement ratios at DNA damage and cell survival endpoints in radiation therapy

2020 ◽  
Vol 65 (9) ◽  
pp. 095006
Author(s):  
Yusuke Matsuya ◽  
Tatsuhiko Sato ◽  
Rui Nakamura ◽  
Shingo Naijo ◽  
Hiroyuki Date
Keyword(s):  
Dna Damage ◽  
Radiation Therapy ◽  
Cell Survival ◽  
Cell Killing ◽  
Survival Endpoints ◽  
Oxygen Enhancement
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