Gluconeogenesis is reduced from alanine, lactate and pyruvate, but maintained from glycerol, in liver perfusion of rats with early and late sepsis

2021 ◽  
Author(s):  
Winny Beatriz Souza Galia ◽  
Giuliana Regina Biazi ◽  
Isabele Gonçalves Frasson‐Uemura ◽  
Daniele Romani Miksza ◽  
Cássia Thaïs Bussamra Vieira Zaia ◽  
...  
Keyword(s):  
Liver Perfusion ◽  
Lactate And Pyruvate

Effects of acute exercise on the gluconeogenic capacity of periportal and perivenous hepatocytes

2001 ◽  
Vol 91 (3) ◽  
pp. 1099-1104 ◽  
Author(s):  
François Désy ◽  
Yan Burelle ◽  
Patrice Bélanger ◽  
Marielle Gascon-Barré ◽  
Jean-Marc Lavoie
Keyword(s):  
Incubation Medium ◽  
Acute Exercise ◽  
Metabolic Response ◽  
Liver Perfusion ◽  
Glucose Production ◽  
Selective Destruction ◽  
Single Bout ◽  
Lactate And Pyruvate ◽  
The Difference ◽  
Exercise Induced

The present study was conducted to examine the effect of a single bout of exercise (rodent treadmill, 60 min at 26 m/min, 0% grade) on the gluconeogenic activity of periportal hepatocytes (PP-H) and perivenous hepatocytes (PV-H) in fasted (18 h) rats. Isolated PP-H and PV-H, obtained by selective destruction following liver perfusion with digitonin and collagenase, were incubated with saturating concentrations of alanine (Ala; 20 mM) or a mixture of lactate and pyruvate (Lac+Pyr; 20:2 mM) to determine the glucose production flux ( J glucose) in the incubation medium. Results show that, in the resting conditions, J glucose from all exogenous substrates was significantly higher ( P < 0.01) in PP-H than in PV-H. Exercise, compared with rest, resulted in a higher J glucose ( P < 0.01) from Lac+Pyr substrate in the PV-H but not in the PP-H, resulting in the disappearance of the difference in J glucosebetween PP-H and PV-H. Exercise, compared with rest, led to a higher J glucose ( P < 0.01) from Ala substrate in both PP-H and PV-H. However, the exercise-induced increase in J glucose (gluconeogenic activity) from Ala substrate was higher in PV-H than in PP-H, resulting, as from Lac+Pyr substrate, in the disappearance ( P > 0.05) of the difference of J glucose between PP-H and PV-H. It is concluded that exercise differentially stimulates the gluconeogenic activity of PV-H to a larger extent than PP-H, indicative of a heterogenous metabolic response of hepatocytes to exercise.

scholarly journals Carbon-14 Tracer Studies in Rat-Liver Perfusion Experiments under Conditions of Gluconeogenesis from Lactate and Pyruvate

1977 ◽  
Vol 75 (2) ◽  
pp. 319-330 ◽  
Author(s):  
Gerd MULLHOFER ◽  
Andreas SCHWAB ◽  
Claudia MULLER ◽  
Christel STETTEN ◽  
Edwige GRUBER
Keyword(s):  
Rat Liver ◽  
Liver Perfusion ◽  
Tracer Studies ◽  
Rat Liver Perfusion ◽  
Carbon 14 ◽  
Lactate And Pyruvate

scholarly journals Regulation of glycogen synthesis from glucose and gluconeogenic precursors by insulin in periportal and perivenous rat hepatocytes

1990 ◽  
Vol 266 (1) ◽  
pp. 91-102 ◽  
Author(s):  
L Agius ◽  
M Peak ◽  
K G M M Alberti
Keyword(s):  
Initial Rate ◽  
Liver Perfusion ◽  
Glycogen Synthesis ◽  
Rat Hepatocytes ◽  
Nutritional State ◽  
Glycogen Accumulation ◽  
Hepatocyte Cultures ◽  
Lactate And Pyruvate ◽  
High Concentrations ◽  
Glycogen Deposition

Glycogen synthesis in hepatocyte cultures is dependent on: (1) the nutritional state of the donor rat, (2) the acinar origin of the hepatocytes, (3) the concentrations of glucose and gluconeogenic precursors, and (4) insulin. High concentrations of glucose (15-25 mM) and gluconeogenic precursors (10 mM-lactate and 1 mM-pyruvate) had a synergistic effect on glycogen deposition in both periportal and perivenous hepatocytes. When hepatocytes were challenged with glucose, lactate and pyruvate in the absence of insulin, glycogen was deposited at a linear rate for 2 h and then reached a plateau. However, in the presence of insulin, the initial rate of glycogen deposition was increased (20-40%) and glycogen deposition continued for more than 4 h. Consequently, insulin had a more marked effect on the glycogen accumulated in the cell after 4 h (100-200% increase) than on the initial rate of glycogen deposition. Glycogen accumulation in hepatocyte cultures prepared from rats that were fasted for 24 h and then re-fed for 3 h before liver perfusion was 2-fold higher than in hepatocytes from rats fed ad libitum and 4-fold higher than in hepatocytes from fasted rats. The incorporation of [14C]lactate into glycogen was 2-4-fold higher in periportal than in perivenous hepatocytes in both the absence and the presence of insulin, whereas the incorporation of [14C]glucose into glycogen was similar in periportal and perivenous hepatocytes in the absence of insulin, but higher in perivenous hepatocytes in the presence of insulin. Rates of glycogen deposition in the combined presence of glucose and gluconeogenic precursors were similar in periportal and perivenous hepatocytes, whereas in the presence of glucose alone, rates of glycogen deposition paralleled the incorporation of [14C]glucose into glycogen and were higher in perivenous hepatocytes in the presence of insulin. It is concluded that periportal and perivenous hepatocytes utilize different substrates for glycogen synthesis, but differences between the two cell populations in the relative utilization of glucose and gluconeogenic precursors are dependent on the presence of insulin and on the nutritional state of the rat.

Sources and prevention of graft infection during long term ex situ liver perfusion

2021 ◽  
Author(s):  
Dilmurodjon Eshmuminov ◽  
Matteo Mueller ◽  
Silvio D. Brugger ◽  
Lucia Bautista Borrego ◽  
Dustin Becker ◽  
...  
Keyword(s):  
Liver Perfusion ◽  
Graft Infection ◽  
Ex Situ

scholarly journals Making timely remedial measures after TACE based on the results of cone-beam CT liver perfusion

2021 ◽  
Vol 38 (1) ◽  
pp. 428-436
Author(s):  
Zhaonan Li ◽  
DeChao Jiao ◽  
Guangyan Si ◽  
Xinwei Han ◽  
Wenguang Zhang ◽  
...  
Keyword(s):  
Cone Beam Ct ◽  
Liver Perfusion ◽  
Cone Beam ◽  
Remedial Measures

scholarly journals LACTATE AND PYRUVATE IN BLOOD AND URINE AFTER EXERCISE

1937 ◽  
Vol 118 (2) ◽  
pp. 427-432
Author(s):  
R.E. Johnson ◽  
H.T. Edwards
Keyword(s):  
Lactate And Pyruvate

scholarly journals Abstract No. 544 Evaluation of software predicting selective liver perfusion for embolization planning

2021 ◽  
Vol 32 (5) ◽  
pp. S148-S149
Author(s):  
F. Deschamps ◽  
A. Gill ◽  
L. Tselikas ◽  
G. McLennan ◽  
K. Karuppasamy ◽  
...  
Keyword(s):  
Liver Perfusion

“Synthesis of coagulation factors during long‐term ex situ liver perfusion”

2021 ◽  
Author(s):  
Dilmurodjon Eshmuminov ◽  
Max Hefti ◽  
Matteo Mueller ◽  
Martin J. Schuler ◽  
Lucia Bautista Borrego ◽  
...  
Keyword(s):  
Liver Perfusion ◽  
Coagulation Factors ◽  
Ex Situ
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