The effect of exogenous ciliary neurotrophic factor on cell cycle and neural differentiation markers of in vitro model cells: New insights for future therapeutic approaches

2021 ◽  
Author(s):  
Maryam Ghasemi ◽  
Effat Alizadeh ◽  
Behzad Fallahi Motlagh ◽  
Nosratollah Zarghami
Keyword(s):  
Cell Cycle ◽  
Neurotrophic Factor ◽  
Neural Differentiation ◽  
In Vitro Model ◽  
Ciliary Neurotrophic Factor ◽  
Therapeutic Approaches ◽  
Differentiation Markers ◽  
Vitro Model

scholarly journals Delivery of Thyronamines (TAMs) to the Brain: A Preliminary Study

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1616
Author(s):  
Nicoletta di Leo ◽  
Stefania Moscato ◽  
Marco Borso' ◽  
Simona Sestito ◽  
Beatrice Polini ◽  
...  
Keyword(s):  
High Performance ◽  
In Vitro Model ◽  
New Drugs ◽  
Therapeutic Approaches ◽  
Neuroprotective Effects ◽  
Pharmacological Response ◽  
Preliminary Study ◽  
Vitro Model ◽  
The Brain

Recent reports highlighted the significant neuroprotective effects of thyronamines (TAMs), a class of endogenous thyroid hormone derivatives. In particular, 3-iodothyronamine (T1AM) has been shown to play a pleiotropic role in neurodegeneration by modulating energy metabolism and neurological functions in mice. However, the pharmacological response to T1AM might be influenced by tissue metabolism, which is known to convert T1AM into its catabolite 3-iodothyroacetic acid (TA1). Currently, several research groups are investigating the pharmacological effects of T1AM systemic administration in the search of novel therapeutic approaches for the treatment of interlinked pathologies, such as metabolic and neurodegenerative diseases (NDDs). A critical aspect in the development of new drugs for NDDs is to know their distribution in the brain, which is fundamentally related to their ability to cross the blood–brain barrier (BBB). To this end, in the present study we used the immortalized mouse brain endothelial cell line bEnd.3 to develop an in vitro model of BBB and evaluate T1AM and TA1 permeability. Both drugs, administered at 1 µM dose, were assayed by high-performance liquid chromatography coupled to mass spectrometry. Our results indicate that T1AM is able to efficiently cross the BBB, whereas TA1 is almost completely devoid of this property.

scholarly journals Repeated H 2 O 2 exposure drives cell cycle progression in an in vitro model of ulcerative colitis

2013 ◽  
Vol 17 (12) ◽  
pp. 1619-1631 ◽  
Author(s):  
Angela Poehlmann ◽  
Kathrin Reissig ◽  
Peter Schönfeld ◽  
Diana Walluscheck ◽  
Antje Schinlauer ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Ulcerative Colitis ◽  
Cell Cycle Progression ◽  
In Vitro Model ◽  
Cycle Progression ◽  
Vitro Model

Low molecular weight compound GSB-106 mimics the cellular effects of BDNF after serum deprivation

2019 ◽  
Vol 489 (5) ◽  
pp. 521-524
Author(s):  
L. F. Zainullina ◽  
T. A. Gudasheva ◽  
Yu. V. Vakhitova ◽  
S. B. Seredenin
Keyword(s):  
Cell Cycle ◽  
In Vitro Model ◽  
S Phase ◽  
Serum Deprivation ◽  
Low Molecular Weight ◽  
Cellular Effects ◽  
Cycle Arrest ◽  
Trophic Activity ◽  
Vitro Model

The in vitro model of serum deprivation shows that the survival of SH-SY5Y neuronal cells is ensured by the intrinsic trophic activity of BDNF loop 4-th mimetic GSB‑106 (10-7 М), which is comparable to that of endogenous neurotrophin (10-9 М). The analysis of the cell cycle and S‑phase showed that GSB‑106, like BDNF, induces cell cycle arrest in the G1 phase, diminishes the number of cells in the S‑phase, reduces the number of apoptotic cells and does not stimulate proliferation.

Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation

2015 ◽  
Vol 43 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Jovana Jasnic-Savovic ◽  
Andrijana Klajn ◽  
Milena Milivojevic ◽  
Marija Mojsin ◽  
Gordana Nikcevic
Keyword(s):  
Neural Differentiation ◽  
In Vitro Model ◽  
Embryonal Carcinoma ◽  
Model System ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Serum Free ◽  
Vitro Model ◽  
Serum Free Conditions

scholarly journals Precision-cut bovine udder slices (PCBUS) as an in-vitro-model of an early phase of infection of bovine mastitis

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Viviane Filor ◽  
Monique Petry ◽  
Jessica Meißner ◽  
Manfred Kietzmann
Keyword(s):  
Infectious Diseases ◽  
Early Phase ◽  
In Vitro Model ◽  
Antimicrobial Agents ◽  
Bovine Mastitis ◽  
Therapeutic Approaches ◽  
New Therapeutic Approaches ◽  
Pharmacological Interactions ◽  
Vitro Model

Abstract Background The aim of this study was to establish precision-cut bovine udder slices (PCBUS) as an in-vitro-model to investigate pathophysiological processes in the early phase of mastitis in order to have the possibility to investigate new therapeutic approaches for the treatment of such udder inflammation in later studies. Furthermore, this model should contribute to substitute in-vivo-experiments. Bovine mastitis is one of the most common and costly infectious diseases in the dairy industry, which is largely associated with the use of antimicrobial agents. Given this problem of antimicrobial resistance, it is essential to step up research into bacterial infectious diseases. Thus, the transfer of the in-vitro-model of precision-cut tissue slices to the bovine udder enables broad research into new therapeutic approaches in this area and can also be used to address issues in basic research or the characterisation of complex pathophysiological processes. Results A stimulation with LPS, PGN or the combination of both substances (LPS:PGN) demonstrates the ability of the PCBUS to react with a significant secretion of IL-1ß, TNF-α and PGE2. Conclusion The slices represent an instrument for investigating pharmacological interactions with udder tissue, which can be useful for studies on pharmacological questions and the understanding of complex pathophysiological processes of infection and inflammation.

scholarly journals Precision-Cut Bovine Udder Slices (PCBUS) as an in-vitro-model of an early phase of infection of bovine mastitis

2020 ◽  
Author(s):  
Viviane Filor ◽  
Monique Petry ◽  
Jessica Meißner ◽  
Manfred Kietzmann
Keyword(s):  
Infectious Diseases ◽  
Early Phase ◽  
In Vitro Model ◽  
Antimicrobial Agents ◽  
Bovine Mastitis ◽  
Therapeutic Approaches ◽  
New Therapeutic Approaches ◽  
Pharmacological Interactions ◽  
Vitro Model

Abstract Background: The aim of this study was the establishment of precision-cut bovine udder slices (PCBUS) as an in-vitro -model to investigate pathophysiological processes in the early phase of mastitis in order to have the possibility to investigate new therapeutic approaches for the treatment of such udder inflammation in later studies. Furthermore, this model should contribute to substitute in-vivo -experiments. Bovine mastitis is one of the most common and costly infectious diseases in the dairy industry, which is largely associated with the use of antimicrobial agents. Given this problem of antimicrobial resistance, it is essential to step up research into bacterial infectious diseases. Thus, the transfer of the in-vitro -model of precision-cut tissue slices to the bovine udder enables broad research into new therapeutic approaches in this area and can also be used to address issues in basic research or the characterization of complex pathophysiological processes. Results: A stimulation with LPS, PGN or the combination of both substances (LPS:PGN) demonstrated the ability of the PCBUS to react with a significant secretion of IL-1ß, TNF-α and PGE 2 . Conclusion: The slices represent an instrument for investigating pharmacological interactions with udder tissue, which can be useful for studies on pharmacological questions and the understanding of complex pathophysiological processes of infection and inflammation.

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