MicroRNA26a inhibits cisplatin‐induced renal tubular epithelial cells apoptosis through suppressing the expression of transient receptor potential channel 6 mediated dynamin‐related protein 1

2019 ◽  
Vol 38 (4) ◽  
pp. 384-391
Author(s):  
Yan Yang ◽  
Zuo‐Lin Li ◽  
Feng‐Mei Wang ◽  
Ri‐Ning Tang ◽  
Yan Tu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Transient Receptor Potential Channel ◽  
Related Protein ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Transient Receptor ◽  
Renal Tubular

scholarly journals HCl-activated neural and epithelial vanilloid receptors (TRPV1) in cat esophageal mucosa

2009 ◽  
Vol 297 (1) ◽  
pp. G135-G143 ◽  
Author(s):  
Ling Cheng ◽  
Suzanne de la Monte ◽  
Jie Ma ◽  
Jie Hong ◽  
Ming Tong ◽  
...  
Keyword(s):  
Substance P ◽  
Epithelial Cells ◽  
Transient Receptor Potential ◽  
Platelet Activating Factor ◽  
Circular Muscle ◽  
Receptor Potential ◽  
Transient Receptor Potential Channel ◽  
Esophageal Mucosa ◽  
Transient Receptor ◽  
Krebs Buffer

To test whether transient receptor potential channel vanilloid subfamily member-1 (TRPV1) mediates acid-induced inflammation in the esophagus, a tubular segment of esophageal mucosa was tied at both ends, forming a sac. The sac was filled with 0.01 N HCl (or Krebs buffer for control) and kept in oxygenated Krebs buffer at 37°C. The medium around the sac (supernatant) was collected after 3 h. Supernatant of the HCl-filled sac abolished contraction of esophageal circular muscle strips in response to electric field stimulation. Contraction was similarly abolished by supernatant of mucosal sac filled with the TRPV1 agonist capsaicin (10−6 M). These effects were reversed by the selective TRPV1 antagonist 5′-iodoresiniferatoxin (IRTX) and by the platelet-activating factor (PAF) receptor antagonist CV9388. Substance P and CGRP levels in mucosa and in supernatant increased in response to HCl, and these increases were abolished by IRTX and by tetrodotoxin (TTX) but not affected by CV9388, indicating that substance P and CGRP are neurally released and PAF independent. In contrast, the increase in PAF was blocked by IRTX but not by TTX. Presence of TRPV1 receptor was confirmed by RT-PCR and by Western blot analysis in whole mucosa and in esophageal epithelial cells enzymatically isolated and sorted by flow cytometry or immunoprecipitated with cytokeratin antibodies. In epithelial cells PAF increased in response to HCl, and the increase was abolished by IRTX. We conclude that HCl-induced activation of TRPV1 receptors in esophageal mucosa causes release of substance P and CGRP from neurons and release of PAF from epithelial cells.

Functional expression of the transient receptor potential channel TRPA1, a sensor for toxic lung inhalants, in pulmonary epithelial cells

2013 ◽  
Vol 206 (3) ◽  
pp. 462-471 ◽  
Author(s):  
Thomas Robert Heinrich Büch ◽  
Eva Anna Maria Schäfer ◽  
Maria-Theresia Demmel ◽  
Ingrid Boekhoff ◽  
Horst Thiermann ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Transient Receptor Potential Channel ◽  
Functional Expression ◽  
Pulmonary Epithelial Cells ◽  
Transient Receptor
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