Catalytic properties of DNA polymerase α activity associated with the heat-stabilized nuclear matrix prepared from HeLa S3 cells

1994 ◽  
Vol 12 (2) ◽  
pp. 129-135 ◽  
Author(s):  
Alberto M. Martelli ◽  
Renato Bareggi ◽  
Paola Narducci
Keyword(s):  
Dna Polymerase ◽  
Nuclear Matrix ◽  
Catalytic Properties ◽  
Dna Polymerase Α ◽  
Hela S3 ◽  
Hela S3 Cells ◽  
Α Activity

Regulation of the synthesis of DNA polymerase-α in regenerating liver by calcium and 1,25-dihydroxyvitamin D3

1989 ◽  
Vol 67 (7) ◽  
pp. 345-351 ◽  
Author(s):  
Marianna Sikorska ◽  
Ian de Belle ◽  
James F. Whitfield ◽  
P. Roy Walker
Keyword(s):  
Dna Polymerase ◽  
Calcium Ions ◽  
Nuclear Matrix ◽  
Critical Stage ◽  
Dihydroxyvitamin D3 ◽  
Dna Polymerase Α ◽  
Enzyme Molecules ◽  
Rat Livers ◽  
Α Activity

Digestion of nuclei from normal or partially hepatectomised rat livers with endogenous nucleases liberated a pool of cryptic DNA polymerase-α activity which had previously gone unrecognised. Most of this activity is released into the supernatant as free enzyme molecules (11S), but a small fraction of it is released as a complex of 16S (probably with DNA primase). About 40% of the enzyme remains in the pellet, which contains undigested chromatin and components of the residual nuclear matrix and nucleoskeletal structures. Virtually all of this remaining activity is extracted by 2.0 M salt. The activity of DNA polymerase-α increases equally in all nuclear fractions during the period of DNA replication in regenerating rat liver. Lowering of the serum calcium level by thyroparathyroidectomy does not affect basal DNA polymerase-α activity, but prevents induction of the enzyme during the later stages of prereplicative development. However, an injection of 1α,25-dihydroxy vitamin D3 into the rat during the first 6 h after partial hepatectomy restores the ability of the hepatocytes to induce DNA polymerase-α activity and initiate DNA synthesis. These results are discussed in terms of the role of calcium ions in the regulation of the critical stage of prereplicative development which commits the cells to DNA replication.Key words: replication, nuclear, matrix, complex, lamina, reductase.

Temperature-dependent association of DNA polymerase α activity with the nuclear matrix

1990 ◽  
Vol 190 (2) ◽  
pp. 227-232 ◽  
Author(s):  
Alberto M. Martelli ◽  
R.Stewart Gilmour ◽  
Elisabetta Falcieri ◽  
Francesco A. Manzoli ◽  
Lucio Cocco
Keyword(s):  
Dna Polymerase ◽  
Nuclear Matrix ◽  
Temperature Dependent ◽  
Dna Polymerase Α ◽  
Α Activity
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