Cellular thiols in rat liver cell lines possessing different growth characteristics

1991 ◽  
Vol 9 (2) ◽  
pp. 125-133 ◽  
Author(s):  
Paola Principe ◽  
Patrick A. Riley ◽  
Trevor F. Slater
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Growth Characteristics

Glucose metabolism by adult hepatocytes in primary culture and by cell lines from rat liver

1978 ◽  
Vol 234 (3) ◽  
pp. C122-C130 ◽  
Author(s):  
D. M. Bissell ◽  
G. A. Levine ◽  
M. J. Bissell
Keyword(s):  
Glucose Metabolism ◽  
Primary Culture ◽  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Time Course ◽  
Primary Cultures ◽  
Functional Change ◽  
Permanent Cell ◽  
Rat Hepatoma

The metabolic fate of [U-14C]glucose has been examined in detail in adult rat hepatocytes in primary monolayer culture, as well as in two permanent cell lines--Buffalo rat liver (BRL) and transplantable rat hepatoma (HTC) cells-derived from normal rat liver and from rat hepatoma, respectively. Under defined conditions of incubation, at a glucose concentration of 5.5 mM, the three types of cultured liver cells exhibited pronounced differences in glucose metabolism. Primary cultures, like the intact liver, differed from the cell lines in consuming relatively small amounts of glucose and converting approximately 50% of the total metabolized glucose to lactate. By contrast, the permantent cell lines consumed glucose at a 40-fold greater rate than did primary cultures, converting 80--90% of the carbohydrate to lactate. Oxidative metabolism of glucose carbon also differed among the three types of liver culture. Of the total [U-14C]glucose consumed, primary cultures converted approximately 30% to labeled CO2 per hour, whereas the liver cell lines converted 5--10%. Finally, glucose metabolism in primary culture exhibited adaptation as hepatocytes aged in culture, shifting progressively toward the pattern exhibited by the permanent cell lines. This change occurred over a time course similar to that for other kinds of functional change in hepatocytes in primary culture and thus may be relevant to the general problem of phenotypic alteration in liver cell culture.

Regulation of retinol-binding protein metabolism in cultured rat liver cell lines

Cell ◽  
1978 ◽  
Vol 15 (3) ◽  
pp. 865-873 ◽  
Author(s):  
John Edgar Smith ◽  
Carmia Borek ◽  
DeWitt S. Goodman
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Protein Metabolism ◽  
Binding Protein ◽  
Retinol Binding Protein

Density-dependent expression of keratins in transformed rat liver cell lines

1986 ◽  
Vol 10 (4) ◽  
pp. 263-270 ◽  
Author(s):  
S TROYANOVSKY ◽  
G BANNIKOV ◽  
R MONTESANO ◽  
J VASILIEV
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Density Dependent

CONCENTRATIONS OF METHYLATED NAPHTHALENES, ANTHRACENES, AND PHENANTHRENES OCCURRING IN CZECH RIVER SEDIMENTS AND THEIR EFFECTS ON TOXIC EVENTS ASSOCIATED WITH CARCINOGENESIS IN RAT LIVER CELL LINES

2007 ◽  
Vol 26 (11) ◽  
pp. 2308 ◽  
Author(s):  
Jan Vondráček ◽  
Lenka Švihálková-Šindlerová ◽  
Kateřina Pěnčíková ◽  
Soňa Marvanová ◽  
Pavel Krčmář ◽  
...  
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
River Sediments

Carboxyl-terminal truncation of apolipoprotein B results in gradual loss of the ability to form buoyant lipoproteins in cultured human and rat liver cell lines

Biochemistry ◽  
1991 ◽  
Vol 30 (22) ◽  
pp. 5616-5621 ◽  
Author(s):  
D. Lesley Graham ◽  
Timothy J. Knott ◽  
Tina C. Jones ◽  
Richard J. Pease ◽  
Clive R. Pullinger ◽  
...  
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Apolipoprotein B ◽  
Gradual Loss ◽  
Carboxyl Terminal

Concentrations of methylated naphthalenes, anthracenes and phenathrenes occurring in Czech river sediments and their effects on toxic events associated with carcinogenesis in rat liver cell lines

2007 ◽  
Vol preprint (2007) ◽  
pp. 1
Author(s):  
Jan Vondracek ◽  
Lenka Svihalkova-Sindlerova ◽  
Katerina Pencikova ◽  
Sona Marvanova ◽  
Pavel Krcmar ◽  
...  
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
River Sediments

Cytotoxicity evaluation and antioxidant enzyme expression related to heavy metals found in tuna by-products meal: An in vitro study in human and rat liver cell lines

2013 ◽  
Vol 65 (7-8) ◽  
pp. 1025-1033 ◽  
Author(s):  
Saber Abdelkader Saïdi ◽  
Mohamed Salah Azaza ◽  
Petra Windmolders ◽  
Jos van Pelt ◽  
Abdelfattah El-Feki
Keyword(s):  
Heavy Metals ◽  
Antioxidant Enzyme ◽  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
Enzyme Expression ◽  
By Products

scholarly journals Effect of microsomally activated AFB1 on GGT activity in 3 rat liver cell lines

1982 ◽  
Vol 45 (6) ◽  
pp. 945-952 ◽  
Author(s):  
M M Manson ◽  
J A Green
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell

Cell of origin of distinct cultured rat liver epithelial cells, as typed by cytokeratin and surface component selective expression

1986 ◽  
Vol 64 (8) ◽  
pp. 788-802 ◽  
Author(s):  
Normand Marceau ◽  
Lucie Germain ◽  
René Goyette ◽  
Micheline Noël ◽  
Henriette Gourdeau
Keyword(s):  
Epithelial Cells ◽  
Cell Lines ◽  
Rat Liver ◽  
Liver Cell ◽  
Oval Cell ◽  
Cell Of Origin ◽  
Sinusoidal Cells ◽  
Tissue Sections ◽  
Differential Reactivity ◽  
Cell Antibody

The cell of origin of the nonparenchymal epithelioid cells that emerge in liver cell cultures is unknown. Cultures of rat hepatocytes and several types of nonparenchymal cells obtained by selective tissue dispersion procedures were typed with monoclonal antibodies to rat liver cytokeratin and vimentin, polyvalent antibodies to cow hoof cytokeratins and porcine lens vimentin, and monoclonal antibodies to surface membrane components of ductular oval cells and hepatocytes. Immunoblot analysis revealed that, in cultured rat liver nonparenchymal epithelial cells, the anti-rat hepatocyte cytokeratin antibody recognized a cytokeratin of relative mass (Mr) 55 000 and the anti-cow hoof cytokeratin antibody reacted with a cytokeratin of Mr 52 000, while the anti-vimentin antibodies detected vimentin in both cultured rat fibroblasts and nonparenchymal epithelial cells. Analyses on the specificity of anti-cytokeratin and anti-vimentin antibodies toward the various cellular structures of liver by double immunofluorescence staining of frozen tissue sections revealed unique reactivity patterns. For example, hepatocytes were only stained with anti-Mr 55 000 cytokeratin antibody, while the sinusoidal cells reacted only with the anti-vimentin antibodies. In contrast, epithelial cells of the bile ductular structures and mesothelial cells of the Glisson capsula reacted with all the anti-cytokeratin and anti-vimentin antibodies. It should be stressed, however, that the reaction of the anti-vimentin antibodies on bile ductular cells was weak. The same analysis on tissue sections using the anti-ductular oval cell antibody revealed that it reacted with bile duct structures but not with the Glisson capsula. The anti-hepatocyte antibody reacted only with the parenchymal cells. The differential reactivity of the anti-cytokeratin and anti-vimentin antibodies with the various liver cell compartments was confirmed in primary cultures of hepatocytes, sinusoidal cells, and bile ductular cells, indicating that the present panel of antibodies to intermediate filament constituants allowed a clear-cut distinction between cultured nonparenchymal epithelial cells, hepatocytes, and sinusoidal cells. Indirect immunofluorescence microscopy on nonfixed and para-formaldehyde-fixed cultured hepatocytes and bile ductular cells further confirmed that both anti-hepatocyte and anti-ductular oval cell antibodies recognized surface-exposed components on the respective cell types. By extending the use of this cell typing assay to three established rat liver cell lines of epithelioid morphology (RLEC, MLC, and T51B), we found that all of them reacted with the anti-cytokeratin antibodies, showing that they were indeed of epithelial cell origin. Moreover, these cells were stained with the anti-vimentin antibodies, which yielded differential reactivity patterns equivalent to those of epithelial cells from either the bile ductular structure or the Glisson capsula. Interestingly, the analysis with the antibodies against the exposed surface components revealed a reaction of the anti-ductular oval cell antibody on T51B cells, but not on the other cell lines. This strongly suggests that the T51B epithelial cell type is of bile ductular origin, while the latter cell lines, which exhibit a mesothelial-like phenotype in culture, most likely originate from the Glisson capsula.

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