Differentiation of myeloid cells in liquid culture: 2. Acute myelocytic leukemia cells

1987 ◽  
Vol 5 (3) ◽  
pp. 157-166 ◽  
Author(s):  
Eric Archimbaud ◽  
Harvey D. Preisler
Keyword(s):  
Liquid Culture ◽  
Myeloid Cells ◽  
Leukemia Cells ◽  
Acute Myelocytic Leukemia ◽  
Myelocytic Leukemia

Low p53 expression of acute myelocytic leukemia cells with t(8;21) chromosome abnormality: Association with low p14ARF expression

2006 ◽  
Vol 30 (4) ◽  
pp. 379-383 ◽  
Author(s):  
Masato Shikami ◽  
Hiroshi Miwa ◽  
Kazuhiro Nishii ◽  
Taiichi Kyo ◽  
Isao Tanaka ◽  
...  
Keyword(s):  
Chromosome Abnormality ◽  
Leukemia Cells ◽  
Acute Myelocytic Leukemia ◽  
P53 Expression ◽  
Myelocytic Leukemia

scholarly journals Human B-lymphocyte antigens expressed by lymphocytic and myelocytic leukemia cells. I. Detection by rabbit antisera.

1976 ◽  
Vol 144 (1) ◽  
pp. 167-178 ◽  
Author(s):  
R Billing ◽  
B Rafizadeh ◽  
I Drew ◽  
G Hartman ◽  
R Gale ◽  
...  
Keyword(s):  
B Cell ◽  
B Lymphocytes ◽  
Cell Lines ◽  
B Lymphocyte ◽  
Lymphocytic Leukemia ◽  
Immunofluorescent Staining ◽  
Leukemia Cells ◽  
Acute Myelocytic Leukemia ◽  
Normal Peripheral Blood ◽  
Myelocytic Leukemia

A previously uncharacterized human B-lymphocyte antigen has been detected by rabbit antisera raised to papain digests of spleen cell membranes. The unabsorbed sera reacted in both cytotoxicity and immunofluorescent tests with normal B lymphocytes and cultured B-cell lines but not with normal T lymphocytes or cultured T-cell lines. The cytotoxicity titers against B cells were as high as 1:32,000, whereas the same sera undiluted were negative against T cells. By immunofluorescent staining 6-14% of unfractionated normal lymphocytes and 48-85% of B-rich lymphocyte preparations were positive. Normal peripheral blood granulocytes, platelets, erythrocytes, and phytohemagglutinin blasts were negative. The antisera reacted with the same high titers against leukemia cells from approximately 70% of the patients with acute lymphocytic leukemia, acute myelocytic leukemia, chronic myelocytic leukemia, and seven of eight cases of chronic lymphocytic leukemia. From absorption studies it appeared that the same antigen was being expressed by leukemia cells and normal B lymphocytes. Using immunofluorescent staining the anti-B-cell antisera were able to detect positive leukemia cells in the bone marrow of patients with advanced leukemia and to monitor the elimination of these cells after chemotherapy. Soluble B-cell antigen was found in the serum of some leukemia and lymphoma patients do but not in normal serum.

CD34 expression on acute myelocytic leukemia cells

1997 ◽  
Vol 21 (5) ◽  
pp. 387-389 ◽  
Author(s):  
Robert B. Geller ◽  
Robert A. Bray
Keyword(s):  
Leukemia Cells ◽  
Acute Myelocytic Leukemia ◽  
Myelocytic Leukemia ◽  
Cd34 Expression

scholarly journals The expression of myeloid-specific antigens on myeloid leukemia cells: correlations with leukemia subclasses and implications for normal myeloid differentiation

Blood ◽  
1983 ◽  
Vol 61 (3) ◽  
pp. 456-463
Author(s):  
ED Ball ◽  
MW Fanger
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Myeloid Leukemia ◽  
Present Report ◽  
Leukemia Cells ◽  
Acute Myelocytic Leukemia ◽  
Specific Cell ◽  
Normal Peripheral Blood ◽  
Myelocytic Leukemia ◽  
Specific Cell Surface

The expression of three distinct myeloid-specific cell surface antigens detected by monoclonal antibodies (PMN 6, PMN 29, and AML-2–23) on acute and chronic myeloid leukemia cells is correlated with blast cell morphology and normal myeloid cell antigen display. In studies on normal peripheral blood cells, monoclonal antibodies PMN 6 and PMN 29 have previously been shown to react exclusively with neutrophils while AML-2–23 reacts with both neutrophils and monocytes. The present report demonstrates that these antigens are absent from blast cells of patients with acute myelocytic leukemia (AML) classified as M1 and M2 in the French-American-British system and chronic myelocytic leukemia in myeloid blast crisis. However, leukemia cells with myelomonocytic morphology (M4) expressed all three antigens, while cells with pure monocytic features (M5) were generally only positive for AML-2–23. Based on the absence of these antigens on both leukemic and normal myeloblasts and granulocyte-monocyte progenitors and their characteristic patterns of display on more differentiated leukemic and normal cells, we propose a modified concept of normal myelopoiesis. In this hypothesis, the myeloblast is an uncommitted cell that gives rise to a series of intermediate precursors that acquire committment to either the granulocytic or monocytic lineage marked by the acquisition of specific cell surface markers.

scholarly journals The expression of myeloid-specific antigens on myeloid leukemia cells: correlations with leukemia subclasses and implications for normal myeloid differentiation

Blood ◽  
1983 ◽  
Vol 61 (3) ◽  
pp. 456-463 ◽  
Author(s):  
ED Ball ◽  
MW Fanger
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Myeloid Leukemia ◽  
Present Report ◽  
Leukemia Cells ◽  
Acute Myelocytic Leukemia ◽  
Specific Cell ◽  
Normal Peripheral Blood ◽  
Myelocytic Leukemia ◽  
Specific Cell Surface

Abstract The expression of three distinct myeloid-specific cell surface antigens detected by monoclonal antibodies (PMN 6, PMN 29, and AML-2–23) on acute and chronic myeloid leukemia cells is correlated with blast cell morphology and normal myeloid cell antigen display. In studies on normal peripheral blood cells, monoclonal antibodies PMN 6 and PMN 29 have previously been shown to react exclusively with neutrophils while AML-2–23 reacts with both neutrophils and monocytes. The present report demonstrates that these antigens are absent from blast cells of patients with acute myelocytic leukemia (AML) classified as M1 and M2 in the French-American-British system and chronic myelocytic leukemia in myeloid blast crisis. However, leukemia cells with myelomonocytic morphology (M4) expressed all three antigens, while cells with pure monocytic features (M5) were generally only positive for AML-2–23. Based on the absence of these antigens on both leukemic and normal myeloblasts and granulocyte-monocyte progenitors and their characteristic patterns of display on more differentiated leukemic and normal cells, we propose a modified concept of normal myelopoiesis. In this hypothesis, the myeloblast is an uncommitted cell that gives rise to a series of intermediate precursors that acquire committment to either the granulocytic or monocytic lineage marked by the acquisition of specific cell surface markers.

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