Ferroportin 1 and hephaestin expression in BeWo cell line with different iron treatment

2011 ◽  
Vol 30 (3) ◽  
pp. 249-255 ◽  
Author(s):  
Yan-Qin Li ◽  
Bin Bai ◽  
Xiao-Xiao Cao ◽  
Hong Yan ◽  
Gui-Hua Zhuang
Keyword(s):  
Cell Line ◽  
Bewo Cell ◽  
Bewo Cell Line ◽  
Ferroportin 1 ◽  
Iron Treatment

Ultrastructure of Choriocarcinoma in Vitro

1969 ◽  
Vol 27 ◽  
pp. 362-363
Author(s):  
John C. Garancis ◽  
R. A. Pattillo
Keyword(s):  
Cell Line ◽  
Physiological Function ◽  
Cell System ◽  
Bewo Cell ◽  
Bewo Cells ◽  
Gestational Choriocarcinoma ◽  
Bewo Cell Line

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

442. Functional perspectives of caspase-14 in the human placenta

2008 ◽  
Vol 20 (9) ◽  
pp. 122
Author(s):  
L. J. White ◽  
A. K. Charles ◽  
A. M. Dharmarajan
Keyword(s):  
Cell Line ◽  
Epidermal Differentiation ◽  
Bewo Cell ◽  
Trophoblast Differentiation ◽  
Cytokeratin 18 ◽  
Bewo Cell Line ◽  
Barrier Formation ◽  
Β Hcg ◽  
Protein Transcription ◽  
Insight Into

The functional barrier for exchange between the mother and fetus in the placenta is created by the fusion of cytotrophoblasts with one another to form a continuous, multinuclear syncytiotrophoblast, which is maintained by the incorporation of underlying proliferative cytotrophoblasts. Disruption to this process has been hypothesised to be involved in the aetiology of preeclampsia. Recently we investigated caspase-14 in the context of trophoblast differentiation as it is crucial to epidermal differentiation and keratin stabilisation, revealing disparate expression of caspase-14 in the differentiating BeWo cell line. Consequently, further examination as to its functional role in trophoblast differentiation was conducted using RNA Interference (RNAi), with the hypothesis that differentiation would be suppressed following caspase-14 silencing. 100nM siRNA were delivered into the BeWo cell line for 16 h before the addition of 20µM Forskolin. Cultures were incubated for a further 24, 48 or 72 h before the extraction of RNA and protein. Transcription of the trophoblast hormone β-hCG, the endothelial mediator of eNOS, and cytokeratin 18 were found to be increased after both 24 and 48 h of differentiation following silencing, implicating caspase-14 in the regulation of these pathways. As both β-hCG and eNOS are significantly increased with trophoblast differentiation, this indicates that caspase-14 functionally suppresses BeWo differentiation. Furthermore, the differential expression of cytokeratin 18 indicates a conserved role for caspase-14 in keratin homeostasis in barrier formation. In conclusion, the suppression of caspase-14 in the BeWo cell line resulted in augmented differentiation, a trait often observed in preeclampsia. Further investigation of caspase-14 activity would provide important insight into mechanisms of trophoblast differentiation, particularly in relation to preeclampsia.

The BeWo cell line derived from a human placental choriocarcinoma is permissive for respiratory syncytial virus infection

Virus Genes ◽  
2019 ◽  
Vol 55 (3) ◽  
pp. 406-410 ◽  
Author(s):  
M. A. Velázquez-Cervantes ◽  
M. Martínez-Castillo ◽  
L. D. González-García ◽  
T. A. Vargas-Pavía ◽  
M. G. Martínez-Salazar ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Virus Infection ◽  
Cell Line ◽  
Respiratory Syncytial Virus Infection ◽  
Bewo Cell ◽  
Bewo Cell Line ◽  
Syncytial Virus

scholarly journals Cytotoxicity of Endocytosis and Efflux Inhibitors in the BeWo Cell Line

2017 ◽  
Vol 17 (5) ◽  
pp. 1-7 ◽  
Author(s):  
Mansi Shah ◽  
Luke Bourner ◽  
Shariq Ali ◽  
Sanaalarab Al-Enazy ◽  
Erik Rytting
Keyword(s):  
Cell Line ◽  
Bewo Cell ◽  
Bewo Cell Line

scholarly journals Permeability properties of monolayers of the human trophoblast cell line BeWo

1997 ◽  
Vol 273 (5) ◽  
pp. C1596-C1604 ◽  
Author(s):  
Fei Liu ◽  
Michael J. Soares ◽  
Kenneth L. Audus
Keyword(s):  
Linoleic Acid ◽  
Cell Line ◽  
Morphological Changes ◽  
Molecular Size ◽  
Bewo Cell ◽  
Human Trophoblast ◽  
In Vitro System ◽  
Size Dependent ◽  
Bewo Cell Line

The BeWo cell line (b30 clone) has been examined as a potential in vitro system to study transplacental transport. At the light and electron microscope level, the cells were observed to form confluent monolayers on polycarbonate filters in ∼5 days and morphologically resembled the typical human trophoblast. BeWo monolayers developed a modest transepithelial electrical resistance and a molecular size-dependent permeability to hydrophilic passive diffusion markers, fluorescein, and selected fluorescein-labeled dextrans. Linoleic acid permeation across BeWo monolayers was asymmetric, saturable, and inhibited by low temperature and excess competing fatty acid. Forskolin and 8-bromoadenosine 3′,5′-cyclic monophosphate treatments stimulated morphological changes in BeWo cultures and enhanced the asymmetric passage of linoleic acid across the BeWo monolayers while having minimal effects on passive permeability, affirming that the differentiation state of the cells can influence membrane transporters and transmonolayer permeability. The basic permeability properties of the BeWo monolayers suggest that the cells grown on permeable supports may be examined as a convenient in vitro system to evaluate some transplacental transport mechanisms.

scholarly journals Adiponectin promotes syncytialisation of BeWo cell line and primary trophoblast cells

2010 ◽  
Vol 8 (1) ◽  
pp. 128 ◽  
Author(s):  
Delphine Benaitreau ◽  
Esther Santos ◽  
Marie-Christine Leneveu ◽  
Philippe De Mazancourt ◽  
René Pecquery ◽  
...  
Keyword(s):  
Cell Line ◽  
Bewo Cell ◽  
Trophoblast Cells ◽  
Bewo Cell Line

scholarly journals The Presence of Inducible Cytochrome P450 Types 1A1 and 1A2 in the BeWo Cell Line

Placenta ◽  
2003 ◽  
Vol 24 (1) ◽  
pp. 45-52 ◽  
Author(s):  
M.L. Avery ◽  
C.E. Meek ◽  
K.L. Audus
Keyword(s):  
Cytochrome P450 ◽  
Cell Line ◽  
Bewo Cell ◽  
Bewo Cell Line
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