Modulation of protein expression levels and DNA methylation status of breast cancer metastasis genes by anthracycline-based chemotherapy and the demethylating agent decitabine

2011 ◽  
Vol 29 (8) ◽  
pp. 651-659 ◽  
Author(s):  
Ferda Ari ◽  
Rudolf Napieralski ◽  
Engin Ulukaya ◽  
Egemen Dere ◽  
Christoph Colling ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Methylation ◽  
Protein Expression ◽  
Cancer Metastasis ◽  
Methylation Status ◽  
Breast Cancer Metastasis ◽  
Expression Levels ◽  
Demethylating Agent

scholarly journals Reduction of Raf-1 Kinase Inhibitor Protein Expression Correlates with Breast Cancer Metastasis

2005 ◽  
Vol 11 (20) ◽  
pp. 7392-7397 ◽  
Author(s):  
Suzanne Hagan ◽  
Fahd Al-Mulla ◽  
Elizabeth Mallon ◽  
Karin Oien ◽  
Rhona Ferrier ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Cancer Metastasis ◽  
Kinase Inhibitor ◽  
Breast Cancer Metastasis ◽  
Inhibitor Protein

Abstract 1439: Clinical significance of KISS1 protein expression in breast cancer metastasis to brain

2011 ◽  
Author(s):  
Ilya V. Ulasov ◽  
Natalya Kaverina ◽  
Bart Thaci ◽  
Peter Pytel ◽  
Husain Sattar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Protein Expression ◽  
Clinical Significance ◽  
Cancer Metastasis ◽  
Breast Cancer Metastasis

scholarly journals Quantitative and correlation analysis of the DNA methylation and expression of DAPK in breast cancer

2016 ◽  
Author(s):  
Youzhi Zhu ◽  
Shuiqin Li ◽  
Qingshui Wang ◽  
Ling Chen ◽  
Kunlin Wu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Methylation ◽  
Protein Expression ◽  
Methylation Status ◽  
Clinicopathological Characteristics ◽  
Clinicopathological Parameters ◽  
Gene Methylation ◽  
Methylation Rate ◽  
Death Associated Protein Kinase ◽  
Mrna And Protein Expression

Backgound. Death associated protein kinase 1 (DAPK) is an important tumor suppressor kinase involved in the regulation of multiple cellular activities such as apoptosis and autophagy. DNA methylation of DAPK gene was found in various types of cancers and often correlated with the clinicopathological characteristics. However, the mRNA and protein expression of DAPK in the same sample was rarely measured. Thus it was unclear if the correlation between DAPK gene methylation and clinicopathological parameters was due to the loss of DAPK expression. Methods. In this study, the DNA methylation rate, mRNA and protein expression of DAPK was quantitatively detected in 15 pairs of breast cancer patient samples including tumor (T) and adjacent non-tumor (N) tissues. Results. The correlation between DNA methylation rate and mRNA expression, together with the correlation between mRNA and protein expression, was calculated. No correlation was observed between any levels using either the measurement value of each sample or the T/N ratio of each pair. Discussion. These data suggested the DNA methylation status of DAPK did not correlate well with its mRNA or protein expression. Extra caution is needed when interpreting the DNA methylation data of DAPK gene in clinical studies.

scholarly journals FOXP3 Inhibits the Metastasis of Breast Cancer by Downregulating the Expression of MTA1

2021 ◽  
Vol 11 ◽  
Author(s):  
Chenlin Liu ◽  
Jun Han ◽  
Xiaoju Li ◽  
Tonglie Huang ◽  
Yuan Gao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cancer Metastasis ◽  
Significant Negative Correlation ◽  
Breast Cancer Metastasis ◽  
Tumour Suppressor Gene ◽  
Expression Levels

BackgroundFOXP3, as a tumour suppressor gene, has a vital function in inhibiting the metastasis of breast cancer cells, but the mechanisms by which it inhibits metastasis have not been fully elucidated. This study intended to explore a new mechanism by which FOXP3 inhibits breast cancer metastasis.MethodsBioinformatic analysis was performed to identify potential downstream molecules of FOXP3. The function of FOXP3 in inhibiting MTA1 expression at the mRNA and protein levels was verified by real-time PCR and Western blot analysis. The interaction between FOXP3 and the MTA1 promoter was verified by transcriptomic experiments. In vitro and in vivo experiments were used to determine whether the regulation of MTA1 by FOXP3 affected the invasion and migration of breast cancer cells. Immunohistochemistry was adopted to explore the correlation between the expression levels of FOXP3 and MTA1 in breast cancer samples.ResultsBioinformatics-based sequencing suggested that MTA1 is a potential downstream molecule of FOXP3. FOXP3 downregulated the expression of MTA1 in breast cancer cells by directly inhibiting MTA1 promoter activity. Importantly, FOXP3’s regulation of MTA1 affected the ability of breast cancer cells to invade and metastasize in vitro and in vivo. Moreover, analysis of clinical specimens showed a significant negative correlation between the expression levels of FOXP3 and MTA1 in breast cancer.ConclusionWe systematically explored a new mechanism by which FOXP3 inhibits breast cancer metastasis via the FOXP3-MTA1 pathway.

scholarly journals Quantitative and correlation analysis of the DNA methylation and expression of DAPK in breast cancer

2016 ◽  
Author(s):  
Youzhi Zhu ◽  
Shuiqin Li ◽  
Qingshui Wang ◽  
Ling Chen ◽  
Kunlin Wu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Methylation ◽  
Protein Expression ◽  
Methylation Status ◽  
Clinicopathological Characteristics ◽  
Clinicopathological Parameters ◽  
Gene Methylation ◽  
Methylation Rate ◽  
Death Associated Protein Kinase ◽  
Mrna And Protein Expression

Backgound. Death associated protein kinase 1 (DAPK) is an important tumor suppressor kinase involved in the regulation of multiple cellular activities such as apoptosis and autophagy. DNA methylation of DAPK gene was found in various types of cancers and often correlated with the clinicopathological characteristics. However, the mRNA and protein expression of DAPK in the same sample was rarely measured. Thus it was unclear if the correlation between DAPK gene methylation and clinicopathological parameters was due to the loss of DAPK expression. Methods. In this study, the DNA methylation rate, mRNA and protein expression of DAPK was quantitatively detected in 15 pairs of breast cancer patient samples including tumor (T) and adjacent non-tumor (N) tissues. Results. The correlation between DNA methylation rate and mRNA expression, together with the correlation between mRNA and protein expression, was calculated. No correlation was observed between any levels using either the measurement value of each sample or the T/N ratio of each pair. Discussion. These data suggested the DNA methylation status of DAPK did not correlate well with its mRNA or protein expression. Extra caution is needed when interpreting the DNA methylation data of DAPK gene in clinical studies.

scholarly journals Quantitative and correlation analysis of the DNA methylation and expression of DAPK in breast cancer

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3084 ◽  
Author(s):  
Youzhi Zhu ◽  
Shuiqin Li ◽  
Qingshui Wang ◽  
Ling Chen ◽  
Kunlin Wu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Methylation ◽  
Protein Expression ◽  
Methylation Status ◽  
Clinicopathological Characteristics ◽  
Clinicopathological Parameters ◽  
Gene Methylation ◽  
Methylation Rate ◽  
Death Associated Protein Kinase ◽  
Mrna And Protein Expression

BackgroundDeath-associated protein kinase 1 (DAPK) is an important tumor suppressor kinase involved in the regulation of multiple cellular activities such as apoptosis and autophagy. DNA methylation of DAPK gene was found in various types of cancers and often correlated with the clinicopathological characteristics. However, the mRNA and protein expression of DAPK in the same sample was rarely measured. Thus, it was unclear if the correlation between DAPK gene methylation and clinicopathological parameters was due to the loss of DAPK expression.MethodsIn this study, the DNA methylation rate, mRNA and protein expression of DAPK was quantitatively detected in 15 pairs of breast cancer patient samples including tumor (T) and adjacent non-tumor (N) tissues.ResultsThe correlation between DNA methylation rate and mRNA expression, together with the correlation between mRNA and protein expression, was calculated. No correlation was observed between any levels using either the measurement value of each sample or the T/N ratio of each pair.DiscussionThese data suggested that the DNA methylation status of DAPK did not correlate well with its mRNA or protein expression. Extra caution is needed when interpreting the DNA methylation data of DAPK gene in clinical studies.

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