The effects of the caffeic acid phenethyl ester(CAPE) on erythrocyte membrane damage after hind limb ischaemia–reperfusion

2004 ◽  
Vol 22 (5) ◽  
pp. 287-290 ◽  
Author(s):  
Lülüfer Tamer ◽  
Nehir Sucu ◽  
Bahadır Ercan ◽  
Ali Ünlü ◽  
Mukadder Çalıkoğlu ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Erythrocyte Membrane ◽  
Hind Limb ◽  
Membrane Damage ◽  
Caffeic Acid Phenethyl Ester ◽  
Limb Ischaemia ◽  
Ischaemia Reperfusion

Potential cytoprotection: antioxidant defence by caffeic acid phenethyl ester against free radical-induced damage of lipids, DNA, and proteins

2008 ◽  
Vol 86 (5) ◽  
pp. 279-287 ◽  
Author(s):  
Ting Wang ◽  
Lixiang Chen ◽  
Weimin Wu ◽  
Yuan Long ◽  
Rui Wang
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Caffeic Acid ◽  
Erythrocyte Membrane ◽  
Polyacrylamide Gel Electrophoresis ◽  
Caffeic Acid Phenethyl Ester ◽  
Water Soluble ◽  
Pbr322 Dna ◽  
Acid Esters

Oxidative stress is considered to be a major cause of cellular injuries in a variety of chronic health problems, such as carcinogenesis and neurodegenerative disorders. Caffeic acid phenethyl ester (CAPE), derived from the propolis of honeybee hives, possesses a variety of biological and pharmacological properties including antioxidant and anticancer activity. In the present study, we focused on the diverse antioxidative functionalities of CAPE and its related polyphenolic acid esters on cellular macromolecules in vitro. The effects on human erythrocyte membrane ghost lipid peroxidation, plasmid pBR322 DNA, and protein damage initiated by the water-soluble initiator 2,2′-azobis(2-amidinopropane) hydrochloride (AAPH) and hydrogen peroxide (H2O2) were monitored by formation of hydroperoxides and by DNA nicking assay, single-cell alkaline electrophoresis, and SDS-polyacrylamide gel electrophoresis. Our results showed that CAPE and its related polyphenolic acid esters elicited remarkable inhibitory effects on erythrocyte membrane lipid peroxidation, cellular DNA strand breakage, and protein fragmentation. The results suggest that CAPE is a potent exogenous cytoprotective and antigenotoxic agent against cell oxidative damage that could be used as a template for designing novel drugs to combat diseases induced by oxidative stress components, such as various types of cancer.

Effect of caffeic acid phenethyl ester on survival of axial pattern flaps in rats with ischaemia–reperfusion injuries

2006 ◽  
Vol 40 (2) ◽  
pp. 73-78 ◽  
Author(s):  
Bilge Türk Bilen ◽  
Hidir Kilinç ◽  
Nezih Alaybeyoglu ◽  
Mehmet Çelik ◽  
Mustafa Iraz ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Ischaemia Reperfusion

Does propofol or caffeic acid phenethyl ester prevent lung injury after hindlimb ischaemia-reperfusion in ventilated rats?

Injury ◽  
2006 ◽  
Vol 37 (5) ◽  
pp. 380-387 ◽  
Author(s):  
Ahmet Akyol ◽  
Hülya Ulusoy ◽  
Mustafa İmamoğlu ◽  
Ali Çay ◽  
Esin Yuluğ ◽  
...  
Keyword(s):  
Lung Injury ◽  
Caffeic Acid ◽  
Caffeic Acid Phenethyl Ester ◽  
Ischaemia Reperfusion

scholarly journals A reproducible method for biochemical, histological and functional assessment of the effects of ischaemia–reperfusion syndrome in the lower limbs

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Iñigo Cearra ◽  
Borja Herrero de la Parte ◽  
Diana Isabel Moreno-Franco ◽  
Ignacio García-Alonso
Keyword(s):  
Hind Limb ◽  
Lower Limbs ◽  
Muscle Fibres ◽  
Limb Ischaemia ◽  
Ischaemia Reperfusion Injury ◽  
Pneumatic Tourniquet ◽  
Cross Sectional ◽  
Ischaemia Reperfusion ◽  
Test Treatments ◽  
Induced Changes

AbstractCurrent methodology described to mimic lower limb ischaemia–reperfusion injury (LL-IRI) does not accurately define the procedures and pressures exerted to induce and maintain ischaemia. In this piece of work, we propose a well-defined and detailed rat model that simulates the conditions established in clinical practice guidelines for tourniquet application and allows us to test treatments that aim to prevent/reduce LL-IRI. Eighty-six male WAG/RijHsd rats were subjected to hind limb IRI (LL-IRI), using a mechanical system applying a 1 kg tension to induce and maintain ischemia for 2 or 3 h, and assessed the damage caused by reperfusion at biochemical and muscular levels at different time points. At the biochemical level, both 2 and 3 h of ischemia induced changes (except for electrolyte levels); 3 h of ischemia induced greater changes in specific markers of muscular damage: creatine kinase (CK) and lactate dehydrogenase (LDH). At the histopathological level, 3 h of ischemia and 24 h of reperfusion was associated with an increase in hind limb girth, cross-sectional area, and weight and presence of neutrophils, as well as histological damage in more than 60% of muscle fibres. Our model allows to reliably reproduce the damage associated with the use of a pneumatic tourniquet. CK and LDH, as well as measures of tissue damage, allow to define and characterize the response to LL-IRI-related damage. A period of 3 h of ischemia followed by 3 h of reperfusion caused only local damage but showed greater sensitivity to detect differences in future studies on prophylactic treatments against LL-IRI.

Neuroprotective effects of tramadol on cerebral injuries caused by hind limb ischaemia/reperfusion in rats

2013 ◽  
Vol 23 (5) ◽  
pp. 1141-1146 ◽  
Author(s):  
Mohammad Ashrafzadeh Takhtfooladi ◽  
Amirali Jahanshahi ◽  
Amir Sotoudeh ◽  
Mohammad Hadi Daneshi ◽  
Kimia Aslani ◽  
...  
Keyword(s):  
Hind Limb ◽  
Limb Ischaemia ◽  
Neuroprotective Effects ◽  
Ischaemia Reperfusion

Novel Caffeic Acid Phenethyl Ester (Cape) Analogues as Inducers of Heme Oxygenase-1

2017 ◽  
Vol 23 (18) ◽  
Author(s):  
Valeria Pittala ◽  
Luca Vanella ◽  
Loredana Salerno ◽  
Claudia Di Giacomo ◽  
Rosaria Acquaviva ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Heme Oxygenase ◽  
Caffeic Acid Phenethyl Ester ◽  
Heme Oxygenase 1
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