Cytoskeletal changes and the system of regulation of alkaline phosphatase activity in human periodontal ligament cells induced by mechanical stress

2004 ◽  
Vol 22 (4) ◽  
pp. 249-256 ◽  
Author(s):  
Mirei Chiba ◽  
Hideo Mitani
Keyword(s):  
Alkaline Phosphatase ◽  
Mechanical Stress ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells

IL-6 Induces Osteoblastic Differentiation of Periodontal Ligament Cells

2008 ◽  
Vol 87 (10) ◽  
pp. 937-942 ◽  
Author(s):  
K. Iwasaki ◽  
M. Komaki ◽  
K. Mimori ◽  
E. Leon ◽  
Y. Izumi ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Ascorbic Acid ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Periodontal Ligament ◽  
Kinase Inhibitors ◽  
Osteoblastic Differentiation ◽  
Mitogen Activated Protein Kinase ◽  
Periodontal Ligament Cells ◽  
Igf I

Interleukin (IL)-6 has been considered as an osteolytic factor involved in periodontal disease. However, the function of IL-6 in osteoblastic differentiation of periodontal ligament cells is not clear. We examined the effects of IL-6 and its soluble receptor (sIL-6R) on osteoblastic differentiation of periodontal ligament cells. Osteoblastic differentiation was induced by ascorbic acid. Osteoblast markers, including alkaline phosphatase activity and Runx2 gene expression, were examined. The mechanism of action of IL-6 on osteoblastic differentiation was evaluated by insulin-like growth factor (IGF)-I production and specific inhibitors for the IL-6-signaling molecule. IL-6/sIL-6R enhanced alkaline phosphatase activity and Runx2. Alkaline phosphatase activity was reduced by anti-IGF-I antibody. Mitogen-activated protein kinase and Janus protein tyrosine kinase inhibitors diminished alkaline phosphatase induced by IL-6/sIL-6R. We conclude that IL-6/sIL-6R increases ascorbic-acid-induced alkaline phosphatase activity through IGF-I production, implying that IL-6 acts not only as an osteolytic factor, but also as a mediator of osteoblastic differentiation in periodontal ligament cells.

scholarly journals Alkaline phosphatase activities of cultured human periodontal ligament cells.

1988 ◽  
Vol 30 (3) ◽  
pp. 208-217 ◽  
Author(s):  
Mitsuhiro OHSHIMA ◽  
Fumiyuki KUWATA ◽  
Kichibee OTSUKA ◽  
Ryozo SAITO ◽  
Kazuki SATO ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Phosphatase Activities ◽  
Human Periodontal Ligament Cells
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