Bioprocess Development for 2,3‐Butanediol Production by  Paenibacillus  Strains

2021 ◽  
Vol 8 (1) ◽  
pp. 44-62
Author(s):  
Daniel Tinôco ◽  
Chrysanthi Pateraki ◽  
Apostolis A. Koutinas ◽  
Denise M. G. Freire
Keyword(s):  
Bioprocess Development

scholarly journals Bioprocess development for enhanced endoglucanase production by newly isolated bacteria, purification, characterization and in-vitro efficacy as anti-biofilm of Pseudomonas aeruginosa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Atef M. Ibrahim ◽  
Ragaa A. Hamouda ◽  
Noura El-Ahmady El-Naggar ◽  
Fatma M. Al-Shakankery
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Yeast Extract ◽  
Statistical Design ◽  
Life Time ◽  
Maximum Activity ◽  
Bioprocess Development ◽  
Ammonium Sulfate Precipitation ◽  
Face Centered ◽  
Central Composite

AbstractEndoglucanase producing bacteria were isolated from Egyptian soils and the most active bacterial strain was identified as Bacillus subtilis strain Fatma/1. Plackett–Burman statistical design was carried out to assess the effect of seven process variables on endoglucanase production. Carboxymethyl cellulose (CMC), yeast extract and peptone were the most significant variables that enhanced the endoglucanase production and thus were selected for further optimization using face-centered central composite design. The highest yield of endoglucanase (32.37 U/mL) was obtained in run no. 9, using 18 g/L CMC, 8 g/L peptone, 7 g/L yeast extract and 0.1 g/L FeSO4.7H2O. The optimized medium showed about eightfold increase in endoglucanase production compared to the unoptimized medium. The produced crude enzyme was further purified by ammonium sulfate precipitation, then DEAE-Sepharose CL6B column. The purified enzyme was shown to have a molecular weight of 37 kDa. The enzyme showed maximum activity at pH 8.0, temperature of 50 °C, incubation time of 60 min. The half-life time (T1/2) was 139.53 min at 50 °C, while being 82.67 min at 60 °C. Endoglucanase at concentration of 12 U/mL effectively removed 84.61% of biofilm matrix of Pseudomonas aeruginosa with marked reduction in carbohydrate content of the biofilm from 63.4 to 7.9 μg.

scholarly journals Simplex-based optimization of numerical and categorical inputs in early bioprocess development: Case studies in HT chromatography

2017 ◽  
Vol 12 (8) ◽  
pp. 1700174 ◽  
Author(s):  
Spyridon Konstantinidis ◽  
Nigel Titchener-Hooker ◽  
Ajoy Velayudhan
Keyword(s):  
Case Studies ◽  
Bioprocess Development

Faster bioprocess development from microscale to bioreactor by the consistent use of controlled feed strategies

2012 ◽  
Vol 29 ◽  
pp. S13
Author(s):  
Peter Neubauer ◽  
Kathleen Szeker ◽  
Jennifer Jaitzig ◽  
Juozas Siurkus
Keyword(s):  
Bioprocess Development
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