Mollicute Anti‐Adhesive and Growth Inhibition Properties of the Methanolic Extract of Propolis from the Brazilian Native Bee Melipona quadrifasciata

2020 ◽  
Author(s):  
Caroline Galgowski ◽  
Sara Pavanelo Frare ◽  
Martinho Rau ◽  
Michele Debiase Alberton ◽  
Sérgio Althoff ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Methanolic Extract ◽  
Melipona Quadrifasciata ◽  
Native Bee

scholarly journals Antibacterial effects of the methanolic extract of Glycine Max (Soybean)

2017 ◽  
Vol 8 (2) ◽  
Author(s):  
Seyede Asma Hosseini Chaleshtori ◽  
Mehrdad Ataie Kachoie ◽  
Seyed Mojtaba Hashemi Jazi
Keyword(s):  
Glycine Max ◽  
Infectious Diseases ◽  
Growth Inhibition ◽  
Pathogenic Bacteria ◽  
Methanolic Extract ◽  
Inhibition Zone ◽  
Penicillin G ◽  
Antibacterial Effects ◽  
Antimicrobial Effects ◽  
Antibiotic Agents

High occurrence of antibiotic resistance in pathogenic bacteria is caused by the use of natural medicinal plants to eliminate risk of infectious diseases. Glycine max is on the most popular and nutritious foods with high antimicrobial effects. The present research was done to study the antibacterial effects of the methanolic extract of G. max against Gram-negative and positive bacteria. G. max seeds of M7 and M9 varieties were purchased and their methanolic extracts were collected. Diameter of the zone of growth inhibition of extracts were measured for pathogenic bacteria using the CLSI guidelines. Antimicrobial effects of G. max extracts were also compared with several antibiotics. Diameter of the inhibition zone of tested bacteria against different treatments of G. max had a ranges of 5.93±0.18 to 22.61±1.80 mm. M7 variety had a higher antimicrobial effects on tested bacteria (P0.05). The highest diameter of the zone of growth inhibition was seen for L. monocytogenes (22.61±1.80 mm) and S. aureus (19.33±1.56 mm). Antimicrobial effects of the methanolic extract of the G. max was also dose-dependent (P0.05). Diameter of the inhibition zone of tested bacteria against different antibiotic agents had a ranges of 5.94±0.38 to 24.95±2.11 mm. Ciprofloxacin, penicillin G tetracycline, gentamicin and azithromycin antibiotic agents exhibited the highest diameter of zone of the growth inhibition for K. pneumoniae (15.71±1.46 mm), L. monocytogenes (23.94±2.03 mm), L. monocytogenes (20.55±1.95 mm), S. aureus (14.27±1.36 mm) and L. monocytogenes (24.95±2.11 mm), respectively. Formulation of antibiotic agent using the 100 mg/mL concentration of the M7 variety of G. max has been recommended for treatment of the cases of infectious diseases

scholarly journals Inhibición del crecimiento de Giardia Lamblia por acción del extracto acuoso y metanólico de semillas de Cucurbita Pepo / Giardia lamblia growth inhibition by action of the aqueous and methanolic extract of cucurbita pepo seed

2015 ◽  
Vol 1 (1) ◽  
pp. 121
Author(s):  
María Porfiria Barrón González ◽  
Ramón Gerardo Rodríguez Garza ◽  
Yadira Quiñones Gutiérrez
Keyword(s):  
Growth Inhibition ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Cucurbita Pepo ◽  
Methanolic Extract ◽  
Taenia Solium

Giardia lamblia es el protozoario parásito causante de la giardiasis, la cual se caracteriza por molestia abdominal, pérdida de peso y desnutrición. La droga de elección para su tratamiento es el metronidazol sin embargo, presenta diversos efectos secundarios adversos en el paciente. Por otra parte, en la herbolaria se conoce a la semilla de Cucurbita pepo por sus propiedades desparasitante, principalmente sobre Entamoeba histolytica y Taenia solium.  Por lo anterior se planteó la hipótesis de que las semillas de C. pepo contienen principios activos capaces de inhibir el crecimiento de G. lamblia bajo condiciones axénicas in vitro. El objetivo de esta investigación fue evaluar la actividad biológica del extracto acuoso y metanólico de las semillas de  C. pepo sobre el crecimiento de G. lamblia.  Tanto el extracto metanólico como el acuoso se identificaron siete grupos funcionales y la actividad antioxidante no fue significativa. En el extracto metanólico se observó mayor efecto giardicida seguido del acuoso, esta diferencia puede deberse a las saponinas que se encuentran sólo en el extracto metanólico. Los resultados sugieren que las semillas contienen principios activos que pueden emplearse para la investigación de nuevos tratamientos para la  giardiasis.

scholarly journals Antimicrobial and anti-oxidant potential of Periploca hydaspidis

2015 ◽  
Vol 10 (3) ◽  
pp. 645 ◽  
Author(s):  
Rafi Ullah ◽  
Jehan Bakht ◽  
Mohammad Shafi
Keyword(s):  
Antioxidant Activity ◽  
Growth Inhibition ◽  
Methanolic Extract ◽  
Maximum Growth ◽  
Zone Of Inhibition ◽  
Aqueous Fraction ◽  
E Coli ◽  
Methanolic Extracts ◽  
Anti Oxidant ◽  
Diffusion Assay

<p>The present study investigates antimicrobial and anti-oxidant potential of different solvent extracted samples from <em>Periploca hydaspidis </em>through disc diffusion assay. The data revealed that all the extracts reduced the growth of the tested microbes using three different concentrations. <em>Pseudomonas aeruginosa </em>and <em>E. coli</em> were sensitive to crude extracts and all fractions measuring varying degree of growth inhibition.  Similarly, the growth of <em>Citrobacter freundii</em> was not inhibited by crude methanolic extracts, n-hexane, ethyl acetate and aqueous extracted samples. Maximum growth inhibition was measured against <em>Klebsiella pneumoniae</em> exposed to aqueous fraction followed by n-hexane fraction. Our results also suggested that among various fungi, <em>Candida albicans</em> was sensitive to crude methanolic extracts, n-hexane and aqueous fractions compared with other fractions. The rest of the fungi under test were resistant to crude and all fractions showing no zone of inhibition. All the extracted samples of the stems and roots showed antioxidant activity, however, crude methanolic extract of stem tissues exhibited better antioxidant activity than the other extracts.</p>

scholarly journals ISOLATION OF CYTOTOXIC CONSTITUENT FROM BIOACTIVITY GUIDED FRACTION OF ALYSICARPUS MONILIFER L. (DC.)

2019 ◽  
pp. 69-77
Author(s):  
PURVI HARISHKUMAR KAKRANI ◽  
HARISH KAKRANI ◽  
MANAN RAVAL
Keyword(s):  
Growth Inhibition ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Betulinic Acid ◽  
Breast Cancer Cells ◽  
Methanolic Extract ◽  
Chloroform Fraction ◽  
Tumor Removal ◽  
Aerial Parts ◽  
Mcf 7

Objective: Alysicarpus monilifer (Family Papilionaceae) has been used in the Indigenous system of medicine in tumor removal. The present study was designed to isolate and identify the constituent responsible for cytotoxic (anti-tumor) effects of the plant Alysicarpus monilifer. Methods: The plant was powdered and extracted to give a methanolic extract. Initially, Hexane, chloroform, ethyl acetate and methanolic fractions of the methanolic extract of the plant were subjected to cytotoxic screening using cell line based assay (MTT assay and NRU assay). The chloroform fraction showed significant cytotoxicity, so it was further subjected to column chromatography, to separate the cytotoxic phytoconstituent. The cell lines selected were breast cancer cells (MCF-7 and MDA-MB-468) and Liver cancer cells (HepG2 and HLE cell). Results were calculated as percentage growth inhibition with respect to untreated (control) cells versus treated cells. Result: A triterpene, Betulinic acid, was isolated from the aerial parts of Alysicarpus monilifer. The cytotoxic activity of the identified compound against MCF-7, MDA-MB-231, HLE and HepG2 cells was also found to be highly significant with 90% growth inhibition. Conclusion: The triterpene was identified to be betulinic acid, to which the cytotoxic activity can be attributed. It is a first report of isolation of betulinic acid from the Alysicarpus species.

scholarly journals Evaluation of Botanicals of Invasive Plant Species and Fungicides against Fungal Pathogens of Forest Nursery

2021 ◽  
Vol 10 (9) ◽  
pp. 152-162
Author(s):  
Ranjana Juwantha Akshit K. Lohan ◽  
Mohit Singh Bisht Jalaj Saxena ◽  
Pooja Kapoor
Keyword(s):  
Disease Management ◽  
Growth Inhibition ◽  
Plant Species ◽  
Fusarium Solani ◽  
Alternaria Alternata ◽  
Invasive Plant ◽  
Methanolic Extract ◽  
Plant Diseases ◽  
Invasive Plant Species ◽  
Ageratina Adenophora

Investigation of plants that possess natural antimicrobial substances for plant protection has been recognized as a promising disease management strategy. Alternaria alternata, Fusarium solani, Macrophomina spp., and Pestalotiopsis spp. isolated from diverse hosts such as Azadirachta indica, Melia dubia, Saraca indica and Quercus leucotrichophora produce important diseases in forest nurseries such as leaf blight, leaf spot, and wilt. In this study, botanicals from two invasive plant species, Ageratina adenophora and Ageratum conyzoides, were prepared and tested against plant diseases. Two botanicals (Methanolic extract obtained from the leaves of these two invasive species) and two fungicides were evaluated for their fungal growth inhibitory effects. At 1.5 percent concentration, the methanolic extract of Ageratina adenophora was found highly effective, inhibiting the growth of Macrophomina spp., (71.94%) followed by Pestalotiopsis spp. (70.20%), Alternaria alternate (51.92%) and Fusarium solani (47.03%). Whereas, Systemic chemical fungicide Thiophanate methylat 1.5% concentration showed maximum mycelial growth inhibition of Alternaria alternata (77.20%) and Macrophomina spp. (82.43%) and being deadlier to Pestalotiopsis spp. (100%) and Fusarium solani (100%). Their comparative analysis showed that higher doses of Ageratina adenophora caused either more or almost equal pathogen growth inhibition than lower doses of Chlorothalonil for certain fungi. Thus, promoting eco-friendly disease management strategies such as botanical control would be beneficial in reducing the need for pesticides.

scholarly journals Biochemical Studies on Bio Extracts as Antioxidant and Antibacterial Activity

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Farid, M, Hanaa, F. M. Ali, Gehan, F. A. Massoud, Sherein S.
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Growth Inhibition ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Tilia Cordata ◽  
Phytochemical Screening ◽  
Total Polyphenols ◽  
Antioxidants Activity ◽  
Biochemical Studies

Biochemical Evaluated of some bio extracts as follows: Chemical composition and Phytochemical screening were determined in Tilia Cordata and Vitex Agnus leaves. The total polyphenols and flavonoids contents of Tilia Cordata leaves have the highest of total polyphenols and flavonoids contents, which were 126.00mgGAE/g and 15.88mgQE/g, followed by Vitex Agnus leaves, which were 119.77mgGAE/g and 13.41mgQE/g, respectively. Methanolic and aqueous extracts of plants leaves were antioxidant activity by used (FRAP, LPO, DPPH and ABTS) The methanolic extract of Tilia Cordata and Vitex Agnus  leaves, have the highest of reducing power which were 1.649 and 1.018 at the concentrations of 80mg/ml, respectively. Also, by used (LPO, DPPH and ABTS), were the highest antioxidants activity for methanolic extract respectively. Moreover, the methanolic extract of Tilia Cordata leaves produced the highest growth inhibition (18 and 17mm) for against Escherichia coli and Bacillus subtillis at 4mg/ml, respectively. While, The methanolic extract of Vitex Agnus produced the medium percentage of growth inhibition (10.5, 9.75 and 10mm) for against Escherichia coli, St.coccus aureus and Bacillus subtillis at 4mg/ml, respectively. And compared with antibiotic. This study concluded that Tilia Cordata and Vitex Agnus  leaves extract has an antioxidant and antibacterial activity.

scholarly journals Determination of phenolic profile by HPLC–ESI-MS/MS, antioxidant activity, in vitro cytotoxicity and anti-herpetic activity of propolis from the Brazilian native bee Melipona quadrifasciata

2019 ◽  
Vol 29 (3) ◽  
pp. 339-350 ◽  
Author(s):  
Sabrina Hochheim ◽  
Alessandro Guedes ◽  
Ligia Faccin-Galhardi ◽  
Daniele Zendrini Rechenchoski ◽  
Carlos Nozawa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
In Vitro Cytotoxicity ◽  
Phenolic Profile ◽  
Esi Ms ◽  
Melipona Quadrifasciata ◽  
Native Bee

scholarly journals Antitumor activity of leaves of Himatanthus drasticus (Mart.) Plumel-Apocynaceae (janaguba) in the treatment of Sarcoma 180 tumor

2010 ◽  
Vol 46 (2) ◽  
pp. 199-203 ◽  
Author(s):  
Eliane Leite de Sousa ◽  
Ana Ruth Sampaio Grangeiro ◽  
Isla Vanessa Gomes Alves Bastos ◽  
Guilherme Carvalho Ribeiro Rodrigues ◽  
Maria Joselice e Silva ◽  
...  
Keyword(s):  
Side Effects ◽  
Antitumor Activity ◽  
Growth Inhibition ◽  
Crude Extract ◽  
Methanolic Extract ◽  
Tumor Growth Inhibition ◽  
Sarcoma 180 ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Low Toxicity

Himatanthus drasticus, also known as janaguba, is used popularly in Brazil's Northeastern region in the treatment of cancer. However, no scientific reports are available. The present study is the first investigation on the antitumor activity of crude methanolic extract from Himatanthus drasticus leaves against Sarcoma 180 tumor and on its side effects including acute oral toxicity. The OECD 423 methodology was used to study acute oral toxicity, and the STOCK methodology to assess antitumor activity. The crude extract showed low toxicity at the tested doses (50, 300 and 2000 mg/kg) administered orally. The histopathological analyses demonstrated alterations in liver lung, spleen and kidney. It also showed activity against Sarcoma 180 tumor in male Swiss albino mice, evidencing tumor growth inhibition of 67.7% and 68% at 300 mg/kg and 400 mg/kg doses, respectively.

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