Polysaccharide from Lycium arabicum : Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes

2020 ◽  
Vol 17 (12) ◽  
Author(s):  
Jawhar Fakhfakh ◽  
Khaled Athmouni ◽  
Hanen Mallek‐Fakhfakh ◽  
Habib Ayedi ◽  
Noureddine Allouche
Keyword(s):  
Oxidative Damage ◽  
Protective Effect ◽  
Antioxidant Activities ◽  
Structural Features ◽  
Human Erythrocytes

scholarly journals In vitro antioxidant activity of olive leaf extract (Olea europaea L.) and its protective effect on oxidative damage in human erythrocytes

Heliyon ◽  
2018 ◽  
Vol 4 (9) ◽  
pp. e00805 ◽  
Author(s):  
Patricia Goldschmidt Lins ◽  
Silvana Marina Piccoli Pugine ◽  
Antonio Márcio Scatolini ◽  
Mariza Pires de Melo
Keyword(s):  
Antioxidant Activity ◽  
Oxidative Damage ◽  
Protective Effect ◽  
Leaf Extract ◽  
Human Erythrocytes ◽  
Olive Leaf ◽  
Olea Europaea L ◽  
Olive Leaf Extract ◽  
In Vitro Antioxidant Activity

In vitro antioxidant activities of Rhodobacter sphaeroides and protective effect on Caco-2 cell line model

2018 ◽  
Vol 103 (2) ◽  
pp. 917-927 ◽  
Author(s):  
Jun An ◽  
Cui Yang ◽  
Zuming Li ◽  
Patricia W. Finn ◽  
David L. Perkins ◽  
...  
Keyword(s):  
Protective Effect ◽  
Cell Line ◽  
Rhodobacter Sphaeroides ◽  
Antioxidant Activities ◽  
Line Model ◽  
Cell Line Model

scholarly journals Evaluation of radical scavenging system in amoeba Chaos carolinense during nutrient deprivation

2017 ◽  
Vol 7 (4) ◽  
pp. 20160113 ◽  
Author(s):  
Yuru Deng ◽  
Edlyn Li-Hui Lee ◽  
Ketpin Chong ◽  
Zakaria A. Almsherqi
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Nucleic Acids ◽  
Cellular Response ◽  
Molecular Mechanisms ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Cellular Responses ◽  
Biological Macromolecules

The frequent appearance of non-lamellar membrane arrangements such as cubic membranes (CMs) in cells under stressed or pathological conditions points to an intrinsic cellular response mechanism. CM represents highly curved, three-dimensional nano-periodic structures that correspond to mathematically well-defined triply periodic minimal surfaces. Specifically, cellular membrane may transform into CM organization in response to pathological, inflammatory and oxidative stress conditions. CM organization, thus, may provide an advantage to cope with various types of stress. The identification of inducible membrane systems, such as in the mitochondrial inner membranes to cubic morphology upon starvation, opens new avenues for understanding the molecular mechanisms of cellular responses to oxidative stress. In this study, we compared the cellular responses of starved and fed amoeba Chaos carolinense to oxidative stress. Food deprivation from C. carolinense induces a significant increase in prooxidants such as superoxide and hydrogen peroxide. Surprisingly, we observed a significant lower rate of biomolecular damage in starved cells (with higher free radicals generation) when compared with fed cells. Specifically, lipid and RNA damages were significantly less in starved cells compared with fed cells. This observation was not due to the upregulation of intracellular antioxidants, as starved amoeba show reduced antioxidant enzymatic activities; however, it could be attributed to CM formation. CM could uptake and retain short segments of nucleic acids (resembles cellular RNA) in vivo and in vitro. Previous results showed that nucleic acids retained within CM sustain a minimal oxidative damage in vitro upon exposure to high level of superoxide. We thus propose that CM may act as a ‘protective’ shelter to minimize the oxidation of biologically essential macromolecules such as RNA. In summary, we examined enzymatic antioxidant activities as well as oxidative damage biomarkers in starved amoeba C. carolinense in correlation with the potential role of CM as an optimal intracellular membrane organization for the protection of biological macromolecules against oxidative damage.

Protective Effect of Estrogens Against Oxidative Damage to Heart and Skeletal Muscle In Vivo and In Vitro

2008 ◽  
Vol 223 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Adam M. Persky ◽  
Pattie S. Green ◽  
Leighann Stubley ◽  
Cynthia O. Howell ◽  
Larissa Zaulyanov ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Oxidative Damage ◽  
Protective Effect

Protective effect of Ugni molinae Turcz against oxidative damage of human erythrocytes

2007 ◽  
Vol 45 (1) ◽  
pp. 130-135 ◽  
Author(s):  
M. Suwalsky ◽  
P. Orellana ◽  
M. Avello ◽  
F. Villena
Keyword(s):  
Oxidative Damage ◽  
Protective Effect ◽  
Human Erythrocytes ◽  
Ugni Molinae

scholarly journals The Cluster [Re6Se8I6]3− Induces Low Hemolysis of Human Erythrocytes in Vitro: Protective Effect of Albumin

2015 ◽  
Vol 16 (1) ◽  
pp. 1728-1735 ◽  
Author(s):  
Edgardo Rojas-Mancilla ◽  
Alexis Oyarce ◽  
Viviana Verdugo ◽  
Zhiping Zheng ◽  
Rodrigo Ramírez-Tagle
Keyword(s):  
Protective Effect ◽  
Human Erythrocytes

scholarly journals Pyridoxine Decreases Oxidative Stress on Human Erythrocyte Membrane Protein in vitro

2019 ◽  
Vol 13 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Margarita Velásquez ◽  
Darío Méndez ◽  
Carlos Moneriz
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Membrane Protein ◽  
Oxidative Damage ◽  
Erythrocyte Membrane ◽  
Cumene Hydroperoxide ◽  
Protein Carbonylation ◽  
Human Erythrocytes ◽  
Red Cell Membrane

Background: Pyridoxine has reduction and prevention against the levels of reactive oxygen species in in vitro studies. However, the biochemical mechanism that explains this behavior has not yet been fully clarified. Objective: To evaluate the effect of pyridoxine against oxidative damage on the membrane of human erythrocytes. Methods: Cumene hydroperoxide was used to induce oxidative stress in protein and lipid. Human erythrocytes were incubated with pyridoxine and cumene hydroperoxide, either alone or together for 8 h. Oxidative damage was determined by measuring lipid peroxidation and membrane protein carbonylation. Results: The results indicate that the malondialdehyde concentration decreased with increasing concentration of pyridoxine. The membrane protein content also decreased with increasing concentration of vitamin B6, which was confirmed by the decreased signal intensity in the western blot when compared to control without pyridoxine. Results demonstrate that pyridoxine can significantly decrease lipid peroxidation and protein carbonylation in red cell membrane exposed to high concentrations of oxidant agent. Conclusion: Pyridoxine showed a protective effect against the oxidative stress in human erythrocytes in vitro, inhibiting the carbonylation and the oxidative damage of erythrocyte membrane proteins. To date, such an effect has not yet been reported in terms of protein oxidation.

scholarly journals Antioxidant capacity of Melissa Officinalis L. on Biological Systems

2018 ◽  
Vol 43 (3) ◽  
pp. 19
Author(s):  
Juliana Metzner Franco ◽  
Silvana Marina Piccoli Pugine ◽  
Antônio Márcio Scatoline ◽  
Mariza Pires De Melo
Keyword(s):  
Ascorbic Acid ◽  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Protective Action ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Melissa Officinalis ◽  
Frap Assay

The aim of the present study was to evaluate in vitro antioxidant capacity of Melissa extract (ME) (Melissa officinalis L.) and its protective effect on peroxyl radical-induced oxidative damage in erythrocytes. ME used in present study was obtained by rota-evaporation of the crude extract (ethanol:water/dried leaves). Total phenolic and flavonoids contend determination, 176.8 ± 13.2 mg GAE/g dw and  26.2 ± 3.2 mg QE/g dw, respectively).  Total equivalent antioxidant activities, TEAC in mg TE/g dw, were 61.4 ± 5.5 and 512.4 ± 77.2 for respective FRAP assay and DPPH• radical-scavenging. The ME acts as an antioxidant on NO and O2•-, when ME exerted a higher antioxidant action on NO scavenging to compared to the ascorbic acid (1.9 times), however, the antioxidant capacity of ME on O2•- was lower than ascorbic acid (5.6 times). The values of hemolysis inhibition from ME (IC50, 2.0 ± 0.5 mg/mL) were higher than ascorbic acid (IC50, 7.1 ± 1.8 mg/mL). Extract of Melissa was able to eliminate biological free radicals, suggesting a potential to prevent oxidative damage in vivo. In fact, the ME exerted protective action on cell membrane lysis in situ.

In vitro evaluation of the protective effect of crocin on human erythrocytes

2021 ◽  
pp. 106738
Author(s):  
José R. Colina ◽  
Mario Suwalsky ◽  
Karla Petit ◽  
David Contreras ◽  
Marcela Manrique-Moreno ◽  
...  
Keyword(s):  
Protective Effect ◽  
Human Erythrocytes ◽  
In Vitro Evaluation
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